Natural Transformation of Campylobacter jejuni Requires Components of a Type II Secretion System
10.1128/jb.185.18.5408-5418.2003
Crossref journal-article
American Society for Microbiology
Journal of Bacteriology (235)
Abstract

ABSTRACT The human pathogen Campylobacter jejuni is one of more than 40 naturally competent bacterial species able to import macromolecular DNA from the environment and incorporate it into their genomes. However, in C. jejuni little is known about the genes involved in this process. We used random transposon mutagenesis to identify genes that are required for the transformation of this organism. We isolated mutants with insertions in 11 different genes; most of the mutants are affected in the DNA uptake stage of transformation, whereas two mutants are affected in steps subsequent to DNA uptake, such as recombination into the chromosome or in DNA transport across the inner membrane. Several of these genes encode proteins homologous to those involved in type II secretion systems, biogenesis of type IV pili, and competence for natural transformation in gram-positive and gram-negative species. Other genes identified in our screen encode proteins unique to C. jejuni or are homologous to proteins that have not been shown to play a role in the transformation in other bacteria.

Bibliography

Wiesner, R. S., Hendrixson, D. R., & DiRita, V. J. (2003). Natural Transformation of Campylobacter jejuni Requires Components of a Type II Secretion System. Journal of Bacteriology, 185(18), 5408–5418.

Authors 3
  1. Rebecca S. Wiesner (first)
  2. David R. Hendrixson (additional)
  3. Victor J. DiRita (additional)
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Dates
Type When
Created 22 years ago (Aug. 29, 2003, 1:45 p.m.)
Deposited 4 years, 1 month ago (July 29, 2021, 2:10 p.m.)
Indexed 1 year ago (Aug. 24, 2024, 7:09 p.m.)
Issued 21 years, 11 months ago (Sept. 15, 2003)
Published 21 years, 11 months ago (Sept. 15, 2003)
Published Print 21 years, 11 months ago (Sept. 15, 2003)
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@article{Wiesner_2003, title={Natural Transformation of Campylobacter jejuni Requires Components of a Type II Secretion System}, volume={185}, ISSN={1098-5530}, url={http://dx.doi.org/10.1128/jb.185.18.5408-5418.2003}, DOI={10.1128/jb.185.18.5408-5418.2003}, number={18}, journal={Journal of Bacteriology}, publisher={American Society for Microbiology}, author={Wiesner, Rebecca S. and Hendrixson, David R. and DiRita, Victor J.}, year={2003}, month=sep, pages={5408–5418} }