Abstract
ABSTRACT Interactions between the MinD and MinE proteins are required for proper placement of the Escherichia coli division septum. The site within MinE that is required for interaction with MinD was mapped by studying the effects of site-directed minE mutations on MinD-MinE interactions in yeast two-hybrid and three-hybrid experiments. This confirmed that the MinE N-terminal domain is responsible for the interaction of MinE with MinD. Mutations that interfered with the interaction defined an extended surface on one face of the α-helical region of the MinE N-terminal domain, consistent with the idea that the MinE-MinD interaction involves formation of a coiled-coil structure by interaction with a complementary helical surface within MinD.
References
22
Referenced
49
-
de Boer, P. A. D., R. E. Crossley, and L. I. Rothfield. 1989. A division inhibitor and a topological specificity factor coded for by the minicell locus determine proper placement of the division septum in E. coli.Cell56:641-649.
(
10.1016/0092-8674(89)90586-2) / Cell (1989) -
Fu, X., Y.-L. Shih, Y. Zhang, and L. I. Rothfield. 2001. The MinE ring required for proper placement of the division site is a mobile structure that changes its cellular location during the Escherichia coli division cycle. Proc. Natl. Acad. Sci. USA98:980-985.
(
10.1073/pnas.98.3.980) / Proc. Natl. Acad. Sci. USA (2001) -
Hale, C., H. Meinhardt, and P. de Boer. 2001. Dynamic localization cycle of the cell division regulator MinE in Escherichia coli.EMBO J.20:1563-1572.
(
10.1093/emboj/20.7.1563) / EMBO J. (2001) -
Hu, Z., E. Gogol, and J. Lutkenhaus. 2002. Dynamic assembly of MinD on phospholipid vesicles regulated by ATP and MinE. Proc. Natl. Acad. Sci. USA99:6761-6766.
(
10.1073/pnas.102059099) / Proc. Natl. Acad. Sci. USA (2002) -
Hu, Z., and J. Lutkenhaus. 2001. Topological regulation of cell division in E. coli: spatiotemporal oscillation of MinD occurs through stimulation of its ATPase activity by MinE and phospholipid. Mol. Cell7:1337-1343.
(
10.1016/S1097-2765(01)00273-8) / Mol. Cell (2001) 10.1128/JB.185.1.196-203.200310.1128/jb.178.17.5080-5085.1996-
Ishidate, K., E. S. Creeger, J. Zrike, S. Deb, B. Glauner, T. J. MacAlister, and L. I. Rothfield. 1986. Isolation of differentiated membrane domains from Escherichia coli and Salmonella typhimurium, including a fraction containing attachment sites between the inner and outer membranes and the murein skeleton of the cell envelope. J. Biol. Chem.261:428-443.
(
10.1016/S0021-9258(17)42490-2) / J. Biol. Chem. (1986) -
King, G. F., S. L. Rowland, B. Pan, J. Mackay, G. Mullen, and L. I. Rothfield. 1999. The dimerization and topological specificity functions of MinE reside in a structurally autonomous C-terminal domain. Mol. Microbiol.31:1161-1169.
(
10.1046/j.1365-2958.1999.01256.x) / Mol. Microbiol. (1999) -
King, G. F., Y.-L. Shih, M. W. Maciejewski, N. P. S. Bains, B. Pan, S. Rowland, G. P. Mullen, and L. I. Rothfield. 2000. Structural basis for the topological specificity function of MinE. Nat. Struct. Biol.7:1013-1017.
(
10.1038/80917) / Nat. Struct. Biol. (2000) 10.1128/JB.185.3.735-749.2003- Miller J. H. 1972. Experiments in molecular genetics. Cold Spring Harbor Laboratory Cold Spring Harbor N.Y.
-
Pichoff, S., B. Vollrath, C. Touriol, and J.-P. Bouché. 1995. Deletion analysis of gene minE which encodes the topological specificity factor of cell division in Escherichia coli.Mol. Microbiol.18:321-329.
(
10.1111/j.1365-2958.1995.mmi_18020321.x) / Mol. Microbiol. (1995) 10.1128/JB.181.20.6419-6424.1999-
Raskin, D., and P. de Boer. 1997. The MinE ring: an FtsZ-independent cell structure required for selection of the correct division site in E. coli.Cell91:685-694.
(
10.1016/S0092-8674(00)80455-9) / Cell (1997) -
Raskin, D., and P. de Boer. 1999. Rapid pole-to-pole oscillation of a protein required for directing division to the middle of Escherichia coli.Proc. Natl. Acad. Sci. USA96:4971-4976.
(
10.1073/pnas.96.9.4971) / Proc. Natl. Acad. Sci. USA (1999) -
Rothfield, L. I., Y.-L. Shih, and G. F. King. 2001. Polar explorers: membrane proteins that determine division site placement. Cell106:13-16.
(
10.1016/S0092-8674(01)00432-9) / Cell (2001) 10.1128/JB.182.3.613-619.2000-
Shih, Y.-L., X. Fu, G. F. King, T. Le, and L. I. Rothfield. 2002. Division site placement in E. coli: mutations that prevent formation of the MinE ring lead to loss of the normal midcell arrest of growth of polar MinD membrane domains. EMBO J.21:3347-3357.
(
10.1093/emboj/cdf323) / EMBO J. (2002) -
Suefuji, K., R. Valluzzi, and D. RayChaudhuri. 2002. Dynamic assembly of MinD into filament bundles modulated by ATP, phospholipids, and MinE. Proc. Natl. Acad. Sci. USA99:16776-16781.
(
10.1073/pnas.262671699) / Proc. Natl. Acad. Sci. USA (2002) -
Zhang, Y., S. Rowland, G. King, and L. Rothfield. 1998. Relation of the oligomeric structure of MinE to its topological specificity function. Mol. Microbiol.30:265-273.
(
10.1046/j.1365-2958.1998.01059.x) / Mol. Microbiol. (1998) -
Zhao, C.-R., P. de Boer, and L. Rothfield. 1995. Proper placement of the E. coli division site requires two functions that are associated with different domains of the MinE protein. Proc. Natl. Acad. Sci. USA92:4313-4317.
(
10.1073/pnas.92.10.4313) / Proc. Natl. Acad. Sci. USA (1995)
@article{Ma_2003, title={Mapping the MinE Site Involved in Interaction with the MinD Division Site Selection Protein of Escherichia coli}, volume={185}, ISSN={1098-5530}, url={http://dx.doi.org/10.1128/jb.185.16.4948-4955.2003}, DOI={10.1128/jb.185.16.4948-4955.2003}, number={16}, journal={Journal of Bacteriology}, publisher={American Society for Microbiology}, author={Ma, Lu-Yan and King, Glenn and Rothfield, Lawrence}, year={2003}, month=aug, pages={4948–4955} }