Membrane Binding by MinD Involves Insertion of Hydrophobic Residues within the C-Terminal Amphipathic Helix into the Bilayer
10.1128/jb.185.15.4326-4335.2003
Crossref journal-article
American Society for Microbiology
Journal of Bacteriology (235)
Abstract

ABSTRACT MinD binds to phospholipid vesicles in the presence of ATP and is released by MinE, which stimulates the MinD ATPase. Membrane binding requires a short conserved C-terminal region, which has the potential to form an amphipathic helix. This finding has led to a model in which the binding of ATP regulates the formation or accessibility of this helix, which then embeds in the membrane bilayer. To test this model, we replaced each of the four hydrophobic residues within this potential helix with tryptophan or a charged residue. Introduction of a negatively charged amino acid decreased membrane binding of MinD and its ability to activate MinC. In contrast, mutants with tryptophan substitutions retained the ability to bind to the membrane and activate MinC. Fluorescence emission spectroscopy analysis of the tryptophan mutants F263W, L264W, and L267W confirmed that these tryptophan residues did insert into the hydrophobic interior of the bilayer. We conclude that membrane binding by MinD involves penetration of the hydrophobic residues within the C-terminal amphipathic helix into the hydrophobic interior of the bilayer.

Bibliography

Zhou, H., & Lutkenhaus, J. (2003). Membrane Binding by MinD Involves Insertion of Hydrophobic Residues within the C-Terminal Amphipathic Helix into the Bilayer. Journal of Bacteriology, 185(15), 4326–4335.

Authors 2
  1. Huaijin Zhou (first)
  2. Joe Lutkenhaus (additional)
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Dates
Type When
Created 22 years, 1 month ago (July 16, 2003, 6:06 p.m.)
Deposited 4 years ago (July 29, 2021, 2:10 p.m.)
Indexed 1 month, 1 week ago (July 20, 2025, 12:16 a.m.)
Issued 22 years ago (Aug. 1, 2003)
Published 22 years ago (Aug. 1, 2003)
Published Print 22 years ago (Aug. 1, 2003)
Funders 0

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@article{Zhou_2003, title={Membrane Binding by MinD Involves Insertion of Hydrophobic Residues within the C-Terminal Amphipathic Helix into the Bilayer}, volume={185}, ISSN={1098-5530}, url={http://dx.doi.org/10.1128/jb.185.15.4326-4335.2003}, DOI={10.1128/jb.185.15.4326-4335.2003}, number={15}, journal={Journal of Bacteriology}, publisher={American Society for Microbiology}, author={Zhou, Huaijin and Lutkenhaus, Joe}, year={2003}, month=aug, pages={4326–4335} }