DOI: 10.1128/jb.185.13.3795-3803.2003. Identification and Mutational Analysis of Bacteriophage PRD1 Holin Protein P35

Identification and Mutational Analysis of Bacteriophage PRD1 Holin Protein P35

10.1128/jb.185.13.3795-3803.2003

Crossref journal-article

American Society for Microbiology

Journal of Bacteriology (235)

Abstract

ABSTRACT Holin proteins are phage-induced integral membrane proteins which regulate the access of lytic enzymes to host cell peptidoglycan at the time of release of progeny viruses by host cell lysis. We describe the identification of the membrane-containing phage PRD1 holin gene (gene XXXV ). The PRD1 holin protein (P35, 12.8 kDa) acts similarly to its functional counterpart from phage lambda (gene S ), and the defect in PRD1 gene XXXV can be corrected by the presence of gene S of lambda. Several nonsense, missense, and insertion mutations in PRD1 gene XXXV were analyzed. These studies support the overall conclusion that the charged amino acids at the protein C terminus are involved in the timing of host cell lysis.

Bibliography

Rydman, P. S., & Bamford, D. H. (2003). Identification and Mutational Analysis of Bacteriophage PRD1 Holin Protein P35. Journal of Bacteriology, 185(13), 3795â3803.

Authors 2

Pia S. Rydman (first)
Dennis H. Bamford (additional)

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Dates

Type	When
Created	22 years, 2 months ago (June 17, 2003, 1:59 p.m.)
Deposited	4 years ago (July 29, 2021, 2:09 p.m.)
Indexed	1 month, 2 weeks ago (July 2, 2025, 1:11 p.m.)
Issued	22 years, 1 month ago (July 1, 2003)
Published	22 years, 1 month ago (July 1, 2003)
Published Print	22 years, 1 month ago (July 1, 2003)

Funders 0

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BibTeX

@article{Rydman_2003, title={Identification and Mutational Analysis of Bacteriophage PRD1 Holin Protein P35}, volume={185}, ISSN={1098-5530}, url={http://dx.doi.org/10.1128/jb.185.13.3795-3803.2003}, DOI={10.1128/jb.185.13.3795-3803.2003}, number={13}, journal={Journal of Bacteriology}, publisher={American Society for Microbiology}, author={Rydman, Pia S. and Bamford, Dennis H.}, year={2003}, month=jul, pages={3795–3803} }

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