Functional Analysis of HrpF, a Putative Type III Translocon Protein from Xanthomonas campestris pv. vesicatoria
10.1128/jb.184.9.2389-2398.2002
Crossref journal-article
American Society for Microbiology
Journal of Bacteriology (235)
Abstract

ABSTRACT Type III secretion systems (TTSSs) are specialized protein transport systems in gram-negative bacteria which target effector proteins into the host cell. The TTSS of the plant pathogen Xanthomonas campestris pv. vesicatoria, encoded by the hrp (hypersensitive reaction and pathogenicity) gene cluster, is essential for the interaction with the plant. One of the secreted proteins is HrpF, which is required for pathogenicity but dispensable for type III secretion of effector proteins in vitro, suggesting a role in translocation. In this study, complementation analyses of an hrpF null mutant strain using various deletion derivatives revealed the functional importance of the C-terminal hydrophobic protein region. Deletion of the N terminus abolished type III secretion of HrpF. Employing the type III effector AvrBs3 as a reporter, we show that the N terminus of HrpF contains a signal for secretion but not a functional translocation signal. Experiments with lipid bilayers revealed a lipid-binding activity of HrpF as well as HrpF-dependent pore formation. These data indicate that HrpF presumably plays a role at the bacterial-plant interface as part of a bacterial translocon which mediates effector protein delivery across the host cell membrane.

Bibliography

Büttner, D., Nennstiel, D., Klüsener, B., & Bonas, U. (2002). Functional Analysis of HrpF, a Putative Type III Translocon Protein from Xanthomonas campestris pv. vesicatoria. Journal of Bacteriology, 184(9), 2389–2398.

Authors 4
  1. Daniela Büttner (first)
  2. Dirk Nennstiel (additional)
  3. Birgit Klüsener (additional)
  4. Ulla Bonas (additional)
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Dates
Type When
Created 23 years, 1 month ago (July 27, 2002, 6:09 a.m.)
Deposited 4 years, 1 month ago (July 29, 2021, 2:08 p.m.)
Indexed 1 day, 23 hours ago (Aug. 29, 2025, 5:55 a.m.)
Issued 23 years, 3 months ago (May 1, 2002)
Published 23 years, 3 months ago (May 1, 2002)
Published Print 23 years, 3 months ago (May 1, 2002)
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@article{Bu_ttner_2002, title={Functional Analysis of HrpF, a Putative Type III Translocon Protein from Xanthomonas campestris pv. vesicatoria}, volume={184}, ISSN={1098-5530}, url={http://dx.doi.org/10.1128/jb.184.9.2389-2398.2002}, DOI={10.1128/jb.184.9.2389-2398.2002}, number={9}, journal={Journal of Bacteriology}, publisher={American Society for Microbiology}, author={Büttner, Daniela and Nennstiel, Dirk and Klüsener, Birgit and Bonas, Ulla}, year={2002}, month=may, pages={2389–2398} }