Bacterial Outer Membrane Ushers Contain Distinct Targeting and Assembly Domains for Pilus Biogenesis
10.1128/jb.184.22.6260-6269.2002
Crossref journal-article
American Society for Microbiology
Journal of Bacteriology (235)
Abstract

ABSTRACT Biogenesis of a superfamily of surface structures by gram-negative bacteria requires the chaperone/usher pathway, a terminal branch of the general secretory pathway. In this pathway a periplasmic chaperone works together with an outer membrane usher to direct substrate folding, assembly, and secretion to the cell surface. We analyzed the structure and function of the PapC usher required for P pilus biogenesis by uropathogenic Escherichia coli. Structural analysis indicated PapC folds as a β-barrel with short extracellular loops and extensive periplasmic domains. Several periplasmic regions were localized, including two domains containing conserved cysteine pairs. Functional analysis of deletion mutants revealed that the PapC C terminus was not required for insertion of the usher into the outer membrane or for proper folding. The usher C terminus was not necessary for interaction with chaperone-subunit complexes in vitro but was required for pilus biogenesis in vivo. Interestingly, coexpression of PapC C-terminal truncation mutants with the chromosomal fim gene cluster coding for type 1 pili allowed P pilus biogenesis in vivo. These studies suggest that chaperone-subunit complexes target an N-terminal domain of the usher and that subunit assembly into pili depends on a subsequent function provided by the usher C terminus.

Bibliography

Thanassi, D. G., Stathopoulos, C., Dodson, K., Geiger, D., & Hultgren, S. J. (2002). Bacterial Outer Membrane Ushers Contain Distinct Targeting and Assembly Domains for Pilus Biogenesis. Journal of Bacteriology, 184(22), 6260–6269.

Authors 5
  1. David G. Thanassi (first)
  2. Christos Stathopoulos (additional)
  3. Karen Dodson (additional)
  4. Dominik Geiger (additional)
  5. Scott J. Hultgren (additional)
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Dates
Type When
Created 22 years, 10 months ago (Oct. 24, 2002, 4:07 p.m.)
Deposited 4 years ago (July 29, 2021, 2:06 p.m.)
Indexed 2 months, 3 weeks ago (June 4, 2025, 6:29 a.m.)
Issued 22 years, 9 months ago (Nov. 15, 2002)
Published 22 years, 9 months ago (Nov. 15, 2002)
Published Print 22 years, 9 months ago (Nov. 15, 2002)
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@article{Thanassi_2002, title={Bacterial Outer Membrane Ushers Contain Distinct Targeting and Assembly Domains for Pilus Biogenesis}, volume={184}, ISSN={1098-5530}, url={http://dx.doi.org/10.1128/jb.184.22.6260-6269.2002}, DOI={10.1128/jb.184.22.6260-6269.2002}, number={22}, journal={Journal of Bacteriology}, publisher={American Society for Microbiology}, author={Thanassi, David G. and Stathopoulos, Christos and Dodson, Karen and Geiger, Dominik and Hultgren, Scott J.}, year={2002}, month=nov, pages={6260–6269} }