DOI: 10.1128/jb.184.1.67-75.2002. Characterization of a<i>Legionella pneumophila relA</i>Insertion Mutant and Roles of RelA and RpoS in Virulence Gene Expression

Characterization of aLegionella pneumophila relAInsertion Mutant and Roles of RelA and RpoS in Virulence Gene Expression

10.1128/jb.184.1.67-75.2002

Crossref journal-article

American Society for Microbiology

Journal of Bacteriology (235)

Abstract

ABSTRACTTo investigate the involvement of RelA in the regulation ofLegionella pneumophilavirulence, a deletion substitution was constructed in therelAgene. TherelAknockout resulted in an undetectable level of ppGpp in the cells during the stationary phase, but the original level was restored when therelAgene product was supplied on a plasmid. The effect of therelAmutation was examined with two systems that are known to be expressed during the stationary phase inL. pneumophila. Pigment production was found to be dependent on therelAgene product, and only one-half as much pigment was produced by therelAmutant as by the wild-type strain. Flagellum gene expression was also found to be dependent on therelAgene product, as determined with aflaA::lacZfusion. However, therelAgene product was found to be dispensable for intracellular growth both in HL-60-derived human macrophages and in the protozoan hostAcanthamoeba castellanii. To determine the involvement of therelAgene product in expression ofL. pneumophilagenes required for intracellular growth (icm/dotgenes), nineicm::lacZfusions were constructed, and expression of these fusions in the wild-type strain was compared with their expression inrelAmutant strains. Expression of only one of theicm::lacZfusions was moderately reduced in therelAmutant strain. Expression of the nineicm::lacZfusions was also examined in a strain containing an insertion in the gene that codes for the stationary-phase sigma factor RpoS, and similar results were obtained. We concluded that RelA is dispensable for intracellular growth ofL. pneumophilain the two hosts examined and that both RelA and RpoS play minor roles inL. pneumophila icm/dotgene expression.

Bibliography

Zusman, T., Gal-Mor, O., & Segal, G. (2002). Characterization of aLegionella pneumophila relAInsertion Mutant and Roles of RelA and RpoS in Virulence Gene Expression. Journal of Bacteriology, 184(1), 67â75.

Authors 3

Tal Zusman (first)
Ohad Gal-Mor (additional)
Gil Segal (additional)

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Dates

Type	When
Created	23 years, 1 month ago (July 27, 2002, 1:58 a.m.)
Deposited	1 year, 7 months ago (Jan. 5, 2024, 1:54 p.m.)
Indexed	4 months, 2 weeks ago (April 15, 2025, 7:31 a.m.)
Issued	23 years, 8 months ago (Jan. 1, 2002)
Published	23 years, 8 months ago (Jan. 1, 2002)
Published Print	23 years, 8 months ago (Jan. 1, 2002)

Funders 0

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BibTeX

@article{Zusman_2002, title={Characterization of aLegionella pneumophila relAInsertion Mutant and Roles of RelA and RpoS in Virulence Gene Expression}, volume={184}, ISSN={1098-5530}, url={http://dx.doi.org/10.1128/jb.184.1.67-75.2002}, DOI={10.1128/jb.184.1.67-75.2002}, number={1}, journal={Journal of Bacteriology}, publisher={American Society for Microbiology}, author={Zusman, Tal and Gal-Mor, Ohad and Segal, Gil}, year={2002}, month=jan, pages={67–75} }

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