DOI: 10.1128/jb.184.1.104-110.2002. The Lytic Enzyme of Bacteriophage PRD1 Is Associated with the Viral Membrane

The Lytic Enzyme of Bacteriophage PRD1 Is Associated with the Viral Membrane

10.1128/jb.184.1.104-110.2002

Crossref journal-article

American Society for Microbiology

Journal of Bacteriology (235)

Abstract

ABSTRACT Bacteriophage PRD1 encodes two proteins (P7 and P15) that are associated with a muralytic activity. Protein P15 is a soluble β-1,4- N -acetylmuramidase that causes phage-induced host cell lysis. We demonstrate here that P15 is also a structural component of the PRD1 virion and that it is connected to the phage membrane. Small viral membrane proteins P20 and P22 modulate incorporation of P15 into the virion and may connect it to the phage membrane. The principal muralytic protein involved in PRD1 DNA entry seems to be the putative lytic transglycosylase protein P7, as the absence of protein P15 did not delay initiation of phage DNA replication in the virus-host system used. The incorporation of two different lytic enzymes into virions may reflect the broad host range of bacteriophage PRD1.

Bibliography

Rydman, P. S., & Bamford, D. H. (2002). The Lytic Enzyme of Bacteriophage PRD1 Is Associated with the Viral Membrane. Journal of Bacteriology, 184(1), 104â110.

Authors 2

Pia S. Rydman (first)
Dennis H. Bamford (additional)

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Dates

Type	When
Created	23 years, 1 month ago (July 27, 2002, 6:01 a.m.)
Deposited	4 years ago (July 29, 2021, 2:03 p.m.)
Indexed	4 months, 1 week ago (April 17, 2025, 6:29 a.m.)
Issued	23 years, 7 months ago (Jan. 1, 2002)
Published	23 years, 7 months ago (Jan. 1, 2002)
Published Print	23 years, 7 months ago (Jan. 1, 2002)

Funders 0

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BibTeX

@article{Rydman_2002, title={The Lytic Enzyme of Bacteriophage PRD1 Is Associated with the Viral Membrane}, volume={184}, ISSN={1098-5530}, url={http://dx.doi.org/10.1128/jb.184.1.104-110.2002}, DOI={10.1128/jb.184.1.104-110.2002}, number={1}, journal={Journal of Bacteriology}, publisher={American Society for Microbiology}, author={Rydman, Pia S. and Bamford, Dennis H.}, year={2002}, month=jan, pages={104–110} }

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