Mechanisms Causing Rapid and Parallel Losses of Ribose Catabolism in Evolving Populations ofEscherichia coliB
10.1128/jb.183.9.2834-2841.2001
Crossref journal-article
American Society for Microbiology
Journal of Bacteriology (235)
Abstract

ABSTRACTTwelve populations ofEscherichia coliB all lostd-ribose catabolic function during 2,000 generations of evolution in glucose minimal medium. We sought to identify the population genetic processes and molecular genetic events that caused these rapid and parallel losses. Seven independent Rbs−mutants were isolated, and their competitive fitnesses were measured relative to that of their Rbs+progenitor. These Rbs−mutants were all about 1 to 2% more fit than the progenitor. A fluctuation test revealed an unusually high rate, about 5 × 10−5per cell generation, of mutation from Rbs+to Rbs−, which contributed to rapid fixation. At the molecular level, the loss of ribose catabolic function involved the deletion of part or all of the ribose operon (rbsgenes). The physical extent of the deletion varied between mutants, but each deletion was associated with an IS150element located immediately upstream of therbsoperon. The deletions apparently involved transposition into various locations within therbsoperon; recombination between the new IS150copy and the one upstream of therbsoperon then led to the deletion of the intervening sequence. To confirm that the beneficial fitness effect was caused by deletion of therbsoperon (and not some undetected mutation elsewhere), we used P1 transduction to restore the functionalrbsoperon to two Rbs−mutants, and we constructed another Rbs−strain by gene replacement with a deletion not involving IS150. All three of these new constructs confirmed that Rbs−mutants have a competitive advantage relative to their Rbs+counterparts in glucose minimal medium. The rapid and parallel evolutionary losses of ribose catabolic function thus involved both (i) an unusually high mutation rate, such that Rbs−mutants appeared repeatedly in all populations, and (ii) a selective advantage in glucose minimal medium that drove these mutants to fixation.

Bibliography

Cooper, V. S., Schneider, D., Blot, M., & Lenski, R. E. (2001). Mechanisms Causing Rapid and Parallel Losses of Ribose Catabolism in Evolving Populations ofEscherichia coliB. Journal of Bacteriology, 183(9), 2834–2841.

Authors 4
  1. Vaughn S. Cooper (first)
  2. Dominique Schneider (additional)
  3. Michel Blot (additional)
  4. Richard E. Lenski (additional)
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Dates
Type When
Created 23 years, 1 month ago (July 27, 2002, 5:58 a.m.)
Deposited 1 year, 7 months ago (Jan. 5, 2024, 6:54 p.m.)
Indexed 3 weeks ago (Aug. 6, 2025, 8:30 a.m.)
Issued 24 years, 3 months ago (May 1, 2001)
Published 24 years, 3 months ago (May 1, 2001)
Published Print 24 years, 3 months ago (May 1, 2001)
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@article{Cooper_2001, title={Mechanisms Causing Rapid and Parallel Losses of Ribose Catabolism in Evolving Populations ofEscherichia coliB}, volume={183}, ISSN={1098-5530}, url={http://dx.doi.org/10.1128/jb.183.9.2834-2841.2001}, DOI={10.1128/jb.183.9.2834-2841.2001}, number={9}, journal={Journal of Bacteriology}, publisher={American Society for Microbiology}, author={Cooper, Vaughn S. and Schneider, Dominique and Blot, Michel and Lenski, Richard E.}, year={2001}, month=may, pages={2834–2841} }