DOI: 10.1128/jb.183.5.1716-1726.2001. VpsR, a Member of the Response Regulators of the Two-Component Regulatory Systems, Is Required for Expression of vps Biosynthesis Genes and EPS ETr -Associated Phenotypes in Vibrio cholerae O1 El Tor

VpsR, a Member of the Response Regulators of the Two-Component Regulatory Systems, Is Required for Expression of vps Biosynthesis Genes and EPS ETr -Associated Phenotypes in Vibrio cholerae O1 El Tor

10.1128/jb.183.5.1716-1726.2001

Crossref journal-article

American Society for Microbiology

Journal of Bacteriology (235)

Abstract

ABSTRACT The rugose colonial variant of Vibrio cholerae O1 El Tor produces an exopolysaccharide (EPS ETr ) that enables the organism to form a biofilm and to resist oxidative stress and the bactericidal action of chlorine. Transposon mutagenesis of the rugose variant led to the identification of vpsR , which codes for a homologue of the NtrC subclass of response regulators. Targeted disruption of vpsR in the rugose colony genetic background yielded a nonreverting smooth-colony morphotype that produced no detectable EPS ETr and did not form an architecturally mature biofilm. Analysis of two genes, vpsA and vpsL , within the vps cluster of EPS ETr biosynthesis genes revealed that their expression is induced above basal levels in the rugose variant, compared to the smooth colonial variant, and requires vpsR . These results show that VpsR functions as a positive regulator of vpsA and vpsL and thus acts to positively regulate EPS ETr production and biofilm formation.

Bibliography

Yildiz, F. H., Dolganov, N. A., & Schoolnik, G. K. (2001). VpsR, a Member of the Response Regulators of the Two-Component Regulatory Systems, Is Required for Expression of vps Biosynthesis Genes and EPS ETr -Associated Phenotypes in Vibrio cholerae O1 El Tor. Journal of Bacteriology, 183(5), 1716â1726.

Authors 3

Fitnat H. Yildiz (first)
Nadia A. Dolganov (additional)
Gary K. Schoolnik (additional)

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Dates

Type	When
Created	23 years, 1 month ago (July 27, 2002, 6:01 a.m.)
Deposited	4 years, 1 month ago (July 29, 2021, 2:02 p.m.)
Indexed	1 month ago (July 24, 2025, 7:52 a.m.)
Issued	24 years, 5 months ago (March 1, 2001)
Published	24 years, 5 months ago (March 1, 2001)
Published Print	24 years, 5 months ago (March 1, 2001)

Funders 0

None

BibTeX

@article{Yildiz_2001, title={VpsR, a Member of the Response Regulators of the Two-Component Regulatory Systems, Is Required for Expression of vps Biosynthesis Genes and EPS ETr -Associated Phenotypes in Vibrio cholerae O1 El Tor}, volume={183}, ISSN={1098-5530}, url={http://dx.doi.org/10.1128/jb.183.5.1716-1726.2001}, DOI={10.1128/jb.183.5.1716-1726.2001}, number={5}, journal={Journal of Bacteriology}, publisher={American Society for Microbiology}, author={Yildiz, Fitnat H. and Dolganov, Nadia A. and Schoolnik, Gary K.}, year={2001}, month=mar, pages={1716–1726} }

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