DOI: 10.1128/jb.183.3.1078-1084.2001. Cytoplasmic Filament-Deficient Mutant of <i>Treponema denticola</i> Has Pleiotropic Defects

Cytoplasmic Filament-Deficient Mutant of Treponema denticola Has Pleiotropic Defects

10.1128/jb.183.3.1078-1084.2001

Crossref journal-article

American Society for Microbiology

Journal of Bacteriology (235)

Abstract

ABSTRACT In Treponema denticola , a ribbon-like structure of cytoplasmic filaments spans the cytoplasm at all stages of the cell division process. Insertional inactivation was used as a first step to determine the function of the cytoplasmic filaments. A suicide plasmid was constructed that contained part of cfpA and a nonpolar erythromycin resistance cassette ( ermF and ermAM ) inserted near the beginning of the gene. The plasmid was electroporated into T. denticola , and double-crossover recombinants which had the chromosomal copy of cfpA insertionally inactivated were selected. Immunoblotting and electron microscopy confirmed the lack of cytoplasmic filaments. The mutant was further analyzed by dark-field microscopy to determine cell morphology and by the binding of two fluorescent dyes to DNA to assess the distribution of cellular nucleic acids. The cytoplasmic filament protein-deficient mutant exhibited pleiotropic defects, including highly condensed chromosomal DNA, compared to the homogeneous distribution of the DNA throughout the cytoplasm in a wild-type cell. Moreover, chains of cells are formed by the cytoplasmic filament-deficient mutant, and those cells show reduced spreading in agarose, which may be due to the abnormal cell length. The chains of cells and the highly condensed chromosomal DNA suggest that the cytoplasmic filaments may be involved in chromosome structure, segregation, or the cell division process in Treponema .

Bibliography

Izard, J., Samsonoff, W. A., & Limberger, R. J. (2001). Cytoplasmic Filament-Deficient Mutant of Treponema denticola Has Pleiotropic Defects. Journal of Bacteriology, 183(3), 1078â1084.

Authors 3

Jacques Izard (first)
William A. Samsonoff (additional)
Ronald J. Limberger (additional)

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Dates

Type	When
Created	23 years ago (July 27, 2002, 6:01 a.m.)
Deposited	4 years ago (July 29, 2021, 2:01 p.m.)
Indexed	1 year, 3 months ago (May 16, 2024, 12:17 p.m.)
Issued	24 years, 6 months ago (Feb. 1, 2001)
Published	24 years, 6 months ago (Feb. 1, 2001)
Published Print	24 years, 6 months ago (Feb. 1, 2001)

Funders 0

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BibTeX

@article{Izard_2001, title={Cytoplasmic Filament-Deficient Mutant of Treponema denticola Has Pleiotropic Defects}, volume={183}, ISSN={1098-5530}, url={http://dx.doi.org/10.1128/jb.183.3.1078-1084.2001}, DOI={10.1128/jb.183.3.1078-1084.2001}, number={3}, journal={Journal of Bacteriology}, publisher={American Society for Microbiology}, author={Izard, Jacques and Samsonoff, William A. and Limberger, Ronald J.}, year={2001}, month=feb, pages={1078–1084} }

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  "abstract": "<jats:title>ABSTRACT</jats:title>\n          <jats:p>\n            In\n            <jats:italic>Treponema denticola</jats:italic>\n            , a ribbon-like structure of cytoplasmic filaments spans the cytoplasm at all stages of the cell division process. Insertional inactivation was used as a first step to determine the function of the cytoplasmic filaments. A suicide plasmid was constructed that contained part of\n            <jats:italic>cfpA</jats:italic>\n            and a nonpolar erythromycin resistance cassette (\n            <jats:italic>ermF</jats:italic>\n            and\n            <jats:italic>ermAM</jats:italic>\n            ) inserted near the beginning of the gene. The plasmid was electroporated into\n            <jats:italic>T. denticola</jats:italic>\n            , and double-crossover recombinants which had the chromosomal copy of\n            <jats:italic>cfpA</jats:italic>\n            insertionally inactivated were selected. Immunoblotting and electron microscopy confirmed the lack of cytoplasmic filaments. The mutant was further analyzed by dark-field microscopy to determine cell morphology and by the binding of two fluorescent dyes to DNA to assess the distribution of cellular nucleic acids. The cytoplasmic filament protein-deficient mutant exhibited pleiotropic defects, including highly condensed chromosomal DNA, compared to the homogeneous distribution of the DNA throughout the cytoplasm in a wild-type cell. Moreover, chains of cells are formed by the cytoplasmic filament-deficient mutant, and those cells show reduced spreading in agarose, which may be due to the abnormal cell length. The chains of cells and the highly condensed chromosomal DNA suggest that the cytoplasmic filaments may be involved in chromosome structure, segregation, or the cell division process in\n            <jats:italic>Treponema</jats:italic>\n            .\n          </jats:p>",
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