DOI: 10.1128/jb.183.19.5788-5792.2001. The Genome of Archaeal Prophage ΨM100 Encodes the Lytic Enzyme Responsible for Autolysis of <i>Methanothermobacter wolfeii</i>

The Genome of Archaeal Prophage ΨM100 Encodes the Lytic Enzyme Responsible for Autolysis of Methanothermobacter wolfeii

10.1128/jb.183.19.5788-5792.2001

Crossref journal-article

American Society for Microbiology

Journal of Bacteriology (235)

Abstract

ABSTRACT Methanothermobacter wolfeii (formerly Methanobacterium wolfei ), a thermophilic methanoarchaeon whose cultures lyse upon hydrogen starvation, carries a defective prophage called ΨM100 on its chromosome. The nucleotide sequence of ΨM100 and its flanking regions was established and compared to that of the previously sequenced phage ΨM2 of Methanothermobacter marburgensis (formerly Methanobacterium thermoautotrophicum Marburg). The ΨM100 genome extends over 28,798 bp, and its borders are defined by flanking 21-bp direct repeats of a pure-AT sequence, which very likely forms the core of the putative attachment site where the crossing over occurred during integration. A large fragment of 2,793 bp, IFa, apparently inserted into ΨM100 but is absent in the genome of ΨM2. The remaining part of the ΨM100 genome showed 70.8% nucleotide sequence identity to the whole genome of ΨM2. Thirty-four open reading frames (ORFs) on the forward strand and one ORF on the reverse strand were identified in the ΨM100 genome. Comparison of ΨM100-encoded ORFs to those encoded by phage ΨM2 and to other known protein sequences permitted the assignment of putative functions to some ORFs. The ORF28 protein of ΨM100 was identified as the previously known autolytic enzyme pseudomurein endoisopeptidase PeiW produced by M. wolfeii .

Bibliography

Luo, Y., Pfister, P., Leisinger, T., & Wasserfallen, A. (2001). The Genome of Archaeal Prophage Î¨M100 Encodes the Lytic Enzyme Responsible for Autolysis of Methanothermobacter wolfeii. Journal of Bacteriology, 183(19), 5788â5792.

Authors 4

Yongneng Luo (first)
Peter Pfister (additional)
Thomas Leisinger (additional)
Alain Wasserfallen (additional)

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Dates

Type	When
Created	23 years, 1 month ago (July 27, 2002, 6:01 a.m.)
Deposited	4 years, 1 month ago (July 29, 2021, 1:59 p.m.)
Indexed	3 months, 4 weeks ago (May 6, 2025, 12:09 a.m.)
Issued	23 years, 11 months ago (Oct. 1, 2001)
Published	23 years, 11 months ago (Oct. 1, 2001)
Published Print	23 years, 11 months ago (Oct. 1, 2001)

Funders 0

None

BibTeX

@article{Luo_2001, title={The Genome of Archaeal Prophage ΨM100 Encodes the Lytic Enzyme Responsible for Autolysis of Methanothermobacter wolfeii}, volume={183}, ISSN={1098-5530}, url={http://dx.doi.org/10.1128/jb.183.19.5788-5792.2001}, DOI={10.1128/jb.183.19.5788-5792.2001}, number={19}, journal={Journal of Bacteriology}, publisher={American Society for Microbiology}, author={Luo, Yongneng and Pfister, Peter and Leisinger, Thomas and Wasserfallen, Alain}, year={2001}, month=oct, pages={5788–5792} }

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