Two Membrane-Associated NiFeS-Carbon Monoxide Dehydrogenases from the Anaerobic Carbon-Monoxide-Utilizing Eubacterium Carboxydothermus hydrogenoformans
10.1128/jb.183.17.5134-5144.2001
Crossref journal-article
American Society for Microbiology
Journal of Bacteriology (235)
Abstract

ABSTRACT Two monofunctional NiFeS carbon monoxide (CO) dehydrogenases, designated CODH I and CODH II, were purified to homogeneity from the anaerobic CO-utilizing eubacterium Carboxydothermus hydrogenoformans . Both enzymes differ in their subunit molecular masses, N-terminal sequences, peptide maps, and immunological reactivities. Immunogold labeling of ultrathin sections revealed both CODHs in association with the inner aspect of the cytoplasmic membrane. Both enzymes catalyze the reaction CO + H 2 O → CO 2 + 2 e − + 2 H + . Oxidized viologen dyes are effective electron acceptors. The specific enzyme activities were 15,756 (CODH I) and 13,828 (CODH II) μmol of CO oxidized min −1 mg −1 of protein (methyl viologen, pH 8.0, 70°C). The two enzymes oxidize CO very efficiently, as indicated by k cat /K m values at 70°C of 1.3 · 10 9 M −1 CO s −1 (CODH I) and 1.7 · 10 9 M −1 CO s −1 (CODH II). The apparent K m values at pH 8.0 and 70°C are 30 and 18 μM CO for CODH I and CODH II, respectively. Acetyl coenzyme A synthase activity is not associated with the enzymes. CODH I (125 kDa, 62.5-kDa subunit) and CODH II (129 kDa, 64.5-kDa subunit) are homodimers containing 1.3 to 1.4 and 1.7 atoms of Ni, 20 to 22 and 20 to 24 atoms of Fe, and 22 and 19 atoms of acid-labile sulfur, respectively. Electron paramagnetic resonance (EPR) spectroscopy revealed signals indicative of [4Fe-4S] clusters. Ni was EPR silent under any conditions tested. It is proposed that CODH I is involved in energy generation and that CODH II serves in anabolic functions.

Bibliography

Svetlitchnyi, V., Peschel, C., Acker, G., & Meyer, O. (2001). Two Membrane-Associated NiFeS-Carbon Monoxide Dehydrogenases from the Anaerobic Carbon-Monoxide-Utilizing Eubacterium Carboxydothermus hydrogenoformans. Journal of Bacteriology, 183(17), 5134–5144.

Authors 4
  1. Vitali Svetlitchnyi (first)
  2. Christine Peschel (additional)
  3. Georg Acker (additional)
  4. Ortwin Meyer (additional)
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Dates
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Created 23 years, 1 month ago (July 27, 2002, 6:01 a.m.)
Deposited 4 years, 1 month ago (July 29, 2021, 1:59 p.m.)
Indexed 3 days, 13 hours ago (Aug. 31, 2025, 6:30 a.m.)
Issued 24 years ago (Sept. 1, 2001)
Published 24 years ago (Sept. 1, 2001)
Published Print 24 years ago (Sept. 1, 2001)
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@article{Svetlitchnyi_2001, title={Two Membrane-Associated NiFeS-Carbon Monoxide Dehydrogenases from the Anaerobic Carbon-Monoxide-Utilizing Eubacterium Carboxydothermus hydrogenoformans}, volume={183}, ISSN={1098-5530}, url={http://dx.doi.org/10.1128/jb.183.17.5134-5144.2001}, DOI={10.1128/jb.183.17.5134-5144.2001}, number={17}, journal={Journal of Bacteriology}, publisher={American Society for Microbiology}, author={Svetlitchnyi, Vitali and Peschel, Christine and Acker, Georg and Meyer, Ortwin}, year={2001}, month=sep, pages={5134–5144} }