DOI: 10.1128/jb.183.14.4210-4216.2001. Indole Can Act as an Extracellular Signal in <i>Escherichia coli</i>

Indole Can Act as an Extracellular Signal in Escherichia coli

10.1128/jb.183.14.4210-4216.2001

Crossref journal-article

American Society for Microbiology

Journal of Bacteriology (235)

Abstract

ABSTRACT Previous work has shown that lacZ fusions to the cysK, astD, tnaB , and gabT genes in Escherichia coli are activated by self-produced extracellular signals. Using a combination of ethyl acetate extraction, reversed-phase C 18 chromatography, and thin-layer chromatography, we have purified an extracellular activating signal from E. coli supernatants. Mass spectrometry revealed a molecule with an m/z peak of 117, consistent with indole. Nuclear magnetic resonance analysis of the purified E. coli factor and synthetic indole revealed identical profiles. Using synthetic indole, a dose-dependent activation was observed with lacZ fusions to the gabT, astD , and tnaB genes. However, cysK :: lacZ and several control fusions were not significantly activated by indole. Conditioned medium prepared from a tnaA (tryptophanase) mutant, deficient in indole production, supported 26 to 41% lower activation of the gabT and astD fusions. The residual level of activation may be due to a second activating signal. Activation of the tnaB :: lacZ fusion was reduced by greater than 70% in conditioned medium from a tnaA mutant.

Bibliography

Wang, D., Ding, X., & Rather, P. N. (2001). Indole Can Act as an Extracellular Signal in Escherichia coli. Journal of Bacteriology, 183(14), 4210â4216.

Authors 3

Dandan Wang (first)
Xuedong Ding (additional)
Philip N. Rather (additional)

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Dates

Type	When
Created	23 years, 1 month ago (July 27, 2002, 6:03 a.m.)
Deposited	4 years, 1 month ago (July 29, 2021, 1:58 p.m.)
Indexed	1 week ago (Aug. 26, 2025, 2:34 a.m.)
Issued	24 years, 1 month ago (July 15, 2001)
Published	24 years, 1 month ago (July 15, 2001)
Published Print	24 years, 1 month ago (July 15, 2001)

Funders 0

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BibTeX

@article{Wang_2001, title={Indole Can Act as an Extracellular Signal in Escherichia coli}, volume={183}, ISSN={1098-5530}, url={http://dx.doi.org/10.1128/jb.183.14.4210-4216.2001}, DOI={10.1128/jb.183.14.4210-4216.2001}, number={14}, journal={Journal of Bacteriology}, publisher={American Society for Microbiology}, author={Wang, Dandan and Ding, Xuedong and Rather, Philip N.}, year={2001}, month=jul, pages={4210–4216} }

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