DOI: 10.1128/jb.183.14.4115-4126.2001. Constitutive Septal Murein Synthesis in <i>Escherichia coli</i> with Impaired Activity of the Morphogenetic Proteins RodA and Penicillin-Binding Protein 2

Constitutive Septal Murein Synthesis in Escherichia coli with Impaired Activity of the Morphogenetic Proteins RodA and Penicillin-Binding Protein 2

10.1128/jb.183.14.4115-4126.2001

Crossref journal-article

American Society for Microbiology

Journal of Bacteriology (235)

Abstract

ABSTRACT The pattern of peptidoglycan (murein) segregation in cells of Escherichia coli with impaired activity of the morphogenetic proteins penicillin-binding protein 2 and RodA has been investigated by the d -cysteine–biotin immunolabeling technique (M. A. de Pedro, J. C. Quintela, J.-V. Höltje, and H. Schwarz, J. Bacteriol. 179:2823–2834, 1997). Inactivation of these proteins either by amdinocillin treatment or by mutations in the corresponding genes, pbpA and rodA , respectively, leads to the generation of round, osmotically stable cells. In normal rod-shaped cells, new murein precursors are incorporated all over the lateral wall in a diffuse manner, being mixed up homogeneously with preexisting material, except during septation, when strictly localized murein synthesis occurs. In contrast, in rounded cells, incorporation of new precursors is apparently a zonal process, localized at positions at which division had previously taken place. Consequently, there is no mixing of new and old murein. Old murein is preserved for long periods of time in large, well-defined areas. We propose that the observed patterns are the result of a failure to switch off septal murein synthesis at the end of septation events. Furthermore, the segregation results confirm that round cells of rodA mutants do divide in alternate, perpendicular planes as previously proposed (K. J. Begg and W. D. Donachie, J. Bacteriol. 180:2564–2567, 1998).

Bibliography

de Pedro, M. A., Donachie, W. D., HoÌltje, J.-V., & Schwarz, H. (2001). Constitutive Septal Murein Synthesis in Escherichia coli with Impaired Activity of the Morphogenetic Proteins RodA and Penicillin-Binding Protein 2. Journal of Bacteriology, 183(14), 4115â4126.

Authors 4

Miguel A. de Pedro (first)
William D. Donachie (additional)
Joachim-Volker Höltje (additional)
Heinz Schwarz (additional)

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Dates

Type	When
Created	23 years, 1 month ago (July 27, 2002, 6:01 a.m.)
Deposited	4 years, 1 month ago (July 29, 2021, 1:58 p.m.)
Indexed	11 months, 2 weeks ago (Sept. 15, 2024, 6:13 p.m.)
Issued	24 years, 1 month ago (July 15, 2001)
Published	24 years, 1 month ago (July 15, 2001)
Published Print	24 years, 1 month ago (July 15, 2001)

Funders 0

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BibTeX

@article{de_Pedro_2001, title={Constitutive Septal Murein Synthesis in Escherichia coli with Impaired Activity of the Morphogenetic Proteins RodA and Penicillin-Binding Protein 2}, volume={183}, ISSN={1098-5530}, url={http://dx.doi.org/10.1128/jb.183.14.4115-4126.2001}, DOI={10.1128/jb.183.14.4115-4126.2001}, number={14}, journal={Journal of Bacteriology}, publisher={American Society for Microbiology}, author={de Pedro, Miguel A. and Donachie, William D. and Höltje, Joachim-Volker and Schwarz, Heinz}, year={2001}, month=jul, pages={4115–4126} }

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