DOI: 10.1128/jb.182.4.956-960.2000. Genetic Evidence of Distinct Physiological Regulation Mechanisms in the ς 54 Pu Promoter of Pseudomonas putida

Genetic Evidence of Distinct Physiological Regulation Mechanisms in the ς 54 Pu Promoter of Pseudomonas putida

10.1128/jb.182.4.956-960.2000

Crossref journal-article

American Society for Microbiology

Journal of Bacteriology (235)

Abstract

ABSTRACT The activity of the toluene-responsive ς 54 Pu promoter of the pWW0 TOL plasmid of Pseudomonas putida is down-regulated in vivo during exponential growth in rich medium and also by the presence of glucose in the culture. Although the Pu promoter already performs poorly during log growth in minimal medium when amended with casamino acids, the addition of glucose further decreased by two- to threefold the accumulation of β-galactosidase in a Pu-lacZ reporter P. putida strain. Since Pu was still down-regulated during exponential growth regardless of glucose addition, it appeared that the carbohydrate separately influenced promoter activity. This notion was supported by the growth-dependent induction pattern of Pu in a ptsN mutant of P. putida , the loss of which makes Pu no longer responsive to repression by glucose. On the other hand, overexpression of the sigma factor ς 54 , known to partially alleviate the exponential silencing of the promoter, did not affect glucose inhibition of Pu . These data indicated that exponential silencing and carbon source-dependent repression are two overlapping but genetically distinguishable mechanisms that adapt Pu to the physiological status of the cells and nutrient availability.

Authors 2

Ildefonso Cases (first)
Víctor de Lorenzo (additional)

References 18 Referenced 34

Assinder S. J. Williams P. A. The TOL plasmids: determinants of the catabolism of toluene and xylenes.Adv. Microb. Physiol.311990169 (10.1016/S0065-2911(08)60119-8) / Adv. Microb. Physiol. / The TOL plasmids: determinants of the catabolism of toluene and xylenes by Assinder S. J. (1990)
Cases I. Pérez-Martı́n J. de Lorenzo V. Involvement of ς54 in exponential silencing of the Pseudomonas putida TOL plasmid Pu promoter.Mol. Microbiol.191996717 (10.1046/j.1365-2958.1996.345873.x) / Mol. Microbiol. / Involvement of ς54 in exponential silencing of the Pseudomonas putida TOL plasmid Pu promoter by Cases I. (1996)
Cases I. Pérez-Martı́n J. de Lorenzo V. The IIANtr (PtsN) protein of Pseudomonas putida mediates C source inhibition of the ς54-dependent Pu promoter of the TOL plasmid.J. Biol. Chem.27419991556215568 (10.1074/jbc.274.22.15562) / J. Biol. Chem. / The IIANtr (PtsN) protein of Pseudomonas putida mediates C source inhibition of the ς54-dependent Pu promoter of the TOL plasmid by Cases I. (1999)
10.1128/jb.175.21.6902-6907.1993
de Lorenzo V. Eltis L. Kessler B. Timmis K. N. Analysis of Pseudomonas gene products using lacIq/Ptrp-lac plasmids and transposons that confer conditional phenotypes.Gene12319931724 (10.1016/0378-1119(93)90533-9) / Gene / Analysis of Pseudomonas gene products using lacI q /Ptrp-lac plasmids and transposons that confer conditional phenotypes by de Lorenzo V. (1993)
de Lorenzo V. Timmis K. N. Analysis and construction of stable phenotypes in Gram-negative bacteria with Tn5- and Tn10-derived minitransposons.Methods Enzymol.2351994386405 (10.1016/0076-6879(94)35157-0) / Methods Enzymol. / Analysis and construction of stable phenotypes in Gram-negative bacteria with Tn5- and Tn10-derived minitransposons by de Lorenzo V. (1994)
10.1128/aem.62.2.601-606.1996
Fernández S. de Lorenzo V. Pérez-Martı́n J. Activation of the transcriptional regulatory XylR of Pseudomonas putida by release of repression between functional domains.Mol. Microbiol.161995205213 (10.1111/j.1365-2958.1995.tb02293.x) / Mol. Microbiol. / Activation of the transcriptional regulatory XylR of Pseudomonas putida by release of repression between functional domains by Fernández S. (1995)
10.1128/jb.176.6.1773-1776.1994
10.1128/jb.172.12.6651-6660.1990
10.1128/jb.171.8.4326-4333.1989
10.1128/jb.176.9.2517-2524.1994
Miller J. H. Experiments in molecular genetics. 1972 Cold Spring Harbor Laboratory Cold Spring Harbor N.Y
Pérez-Martı́n J. de Lorenzo V. In vitro activities of an N-terminal truncated form of XylR, a ς54-dependent transcriptional activator of Pseudomonas putida.J. Mol. Biol.2581996575587 (10.1006/jmbi.1996.0270) / J. Mol. Biol. / In vitro activities of an N-terminal truncated form of XylR, a ς54-dependent transcriptional activator of Pseudomonas putida by Pérez-Martı́n J. (1996)
Ramos J. L. Marqués S. Timmis K. N. Transcriptional control of the Pseudomonas TOL plasmid catabolic operons is achieved through an interplay of host factors and plasmid encoded regulators.Annu. Rev. Microbiol.511997341373 (10.1146/annurev.micro.51.1.341) / Annu. Rev. Microbiol. / Transcriptional control of the Pseudomonas TOL plasmid catabolic operons is achieved through an interplay of host factors and plasmid encoded regulators by Ramos J. L. (1997)
Sawyer M. H. Baumann P. Berman S. M. Canovas J. L. Pathways of D-fructose catabolism in Pseudomonas.Arch. Microbiol.11219774955 (10.1007/BF00446653) / Arch. Microbiol. / Pathways of D-fructose catabolism in Pseudomonas by Sawyer M. H. (1977)
10.1128/jb.178.13.3727-3735.1996
Sze C. C. Shingler V. The alarmone (p)ppGpp mediates physiological-responsive control at the ς54-dependent Po promoter.Mol. Microbiol.31199912171228 (10.1046/j.1365-2958.1999.01264.x) / Mol. Microbiol. / The alarmone (p)ppGpp mediates physiological-responsive control at the ς54-dependent Po promoter by Sze C. C. (1999)

Dates

Type	When
Created	23 years, 1 month ago (July 27, 2002, 6:01 a.m.)
Deposited	4 years, 1 month ago (July 29, 2021, 1:56 p.m.)
Indexed	1 year, 4 months ago (April 7, 2024, 1:12 a.m.)
Issued	25 years, 6 months ago (Feb. 15, 2000)
Published	25 years, 6 months ago (Feb. 15, 2000)
Published Print	25 years, 6 months ago (Feb. 15, 2000)

Funders 0

None

BibTeX

@article{Cases_2000, title={Genetic Evidence of Distinct Physiological Regulation Mechanisms in the ς 54 Pu Promoter of Pseudomonas putida}, volume={182}, ISSN={1098-5530}, url={http://dx.doi.org/10.1128/jb.182.4.956-960.2000}, DOI={10.1128/jb.182.4.956-960.2000}, number={4}, journal={Journal of Bacteriology}, publisher={American Society for Microbiology}, author={Cases, Ildefonso and de Lorenzo, Víctor}, year={2000}, month=feb, pages={956–960} }

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  "abstract": "<jats:title>ABSTRACT</jats:title>\n          <jats:p>\n            The activity of the toluene-responsive \u03c2\n            <jats:sup>54</jats:sup>\n            <jats:italic>Pu</jats:italic>\n            promoter of the pWW0 TOL plasmid of\n            <jats:italic>Pseudomonas putida</jats:italic>\n            is down-regulated in vivo during exponential growth in rich medium and also by the presence of glucose in the culture. Although the\n            <jats:italic>Pu</jats:italic>\n            promoter already performs poorly during log growth in minimal medium when amended with casamino acids, the addition of glucose further decreased by two- to threefold the accumulation of \u03b2-galactosidase in a\n            <jats:italic>Pu-lacZ</jats:italic>\n            reporter\n            <jats:italic>P. putida</jats:italic>\n            strain. Since\n            <jats:italic>Pu</jats:italic>\n            was still down-regulated during exponential growth regardless of glucose addition, it appeared that the carbohydrate separately influenced promoter activity. This notion was supported by the growth-dependent induction pattern of\n            <jats:italic>Pu</jats:italic>\n            in a\n            <jats:italic>ptsN</jats:italic>\n            mutant of\n            <jats:italic>P. putida</jats:italic>\n            , the loss of which makes\n            <jats:italic>Pu</jats:italic>\n            no longer responsive to repression by glucose. On the other hand, overexpression of the sigma factor \u03c2\n            <jats:sup>54</jats:sup>\n            , known to partially alleviate the exponential silencing of the promoter, did not affect glucose inhibition of\n            <jats:italic>Pu</jats:italic>\n            . These data indicated that exponential silencing and carbon source-dependent repression are two overlapping but genetically distinguishable mechanisms that adapt\n            <jats:italic>Pu</jats:italic>\n            to the physiological status of the cells and nutrient availability.\n          </jats:p>",
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