DOI: 10.1128/jb.182.4.1172-1175.2000. Visualization of Phospholipid Domains in <i>Escherichia coli</i> by Using the Cardiolipin-Specific Fluorescent Dye 10- <i>N</i> -Nonyl Acridine Orange

Visualization of Phospholipid Domains in Escherichia coli by Using the Cardiolipin-Specific Fluorescent Dye 10- N -Nonyl Acridine Orange

10.1128/jb.182.4.1172-1175.2000

Crossref journal-article

American Society for Microbiology

Journal of Bacteriology (235)

Abstract

ABSTRACT Cardiolipin (CL)-specific fluorescent dye 10- N -nonyl-acridine orange (NAO) was used to visualize CL distribution in Escherichia coli cells of different phospholipid compositions. In a filamentous mutant containing only anionic phospholipids, green fluorescent spots were observed along the filaments at approximately regular intervals. Three-dimensional image reconstruction obtained by optical sectioning and a deconvolution algorithm revealed NAO-binding domains in the plane of the cell membrane. Substantial red fluorescence emission of bound NAO supported labeling of CL-containing domains. These structures were not found in mutants deficient in CL biosynthesis. The domains were also observed mostly in the septal region and on the poles in cells of normal size with wild-type phospholipid composition.

Bibliography

Mileykovskaya, E., & Dowhan, W. (2000). Visualization of Phospholipid Domains in Escherichia coli by Using the Cardiolipin-Specific Fluorescent Dye 10- N -Nonyl Acridine Orange. Journal of Bacteriology, 182(4), 1172â1175.

Authors 2

Eugenia Mileykovskaya (first)
William Dowhan (additional)

References 20 Referenced 384

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Chang S.-C. Heacock P. N. Mileykovskaya E. Voelker D. R. Dowhan W. Isolation and characterization of the gene (CLS1) encoding cardiolipin synthase in Saccharomyces cerevisiae.J. Biol. Chem.27319981493314941 (10.1074/jbc.273.24.14933) / J. Biol. Chem. / Isolation and characterization of the gene (CLS1) encoding cardiolipin synthase in Saccharomyces cerevisiae by Chang S.-C. (1998)
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Dates

Type	When
Created	23 years, 1 month ago (July 27, 2002, 6:01 a.m.)
Deposited	4 years, 1 month ago (July 29, 2021, 1:56 p.m.)
Indexed	4 weeks, 1 day ago (Aug. 5, 2025, 9 a.m.)
Issued	25 years, 6 months ago (Feb. 15, 2000)
Published	25 years, 6 months ago (Feb. 15, 2000)
Published Print	25 years, 6 months ago (Feb. 15, 2000)

Funders 0

None

BibTeX

@article{Mileykovskaya_2000, title={Visualization of Phospholipid Domains in Escherichia coli by Using the Cardiolipin-Specific Fluorescent Dye 10- N -Nonyl Acridine Orange}, volume={182}, ISSN={1098-5530}, url={http://dx.doi.org/10.1128/jb.182.4.1172-1175.2000}, DOI={10.1128/jb.182.4.1172-1175.2000}, number={4}, journal={Journal of Bacteriology}, publisher={American Society for Microbiology}, author={Mileykovskaya, Eugenia and Dowhan, William}, year={2000}, month=feb, pages={1172–1175} }

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        "name": "publication_history",
        "label": "Publication History"
      }
    },
    {
      "value": "1999-11-29",
      "order": 1,
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      "group": {
        "name": "publication_history",
        "label": "Publication History"
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    },
    {
      "value": "2020-12-20",
      "order": 2,
      "name": "published",
      "label": "Published",
      "group": {
        "name": "publication_history",
        "label": "Publication History"
      }
    }
  ]
}