DOI: 10.1128/jb.182.3.848-854.2000. PpdD Type IV Pilin of <i>Escherichia coli</i> K-12 Can Be Assembled into Pili in <i>Pseudomonas aeruginosa</i>

PpdD Type IV Pilin of Escherichia coli K-12 Can Be Assembled into Pili in Pseudomonas aeruginosa

10.1128/jb.182.3.848-854.2000

Crossref journal-article

American Society for Microbiology

Journal of Bacteriology (235)

Abstract

ABSTRACT Escherichia coli K-12 possesses at least 16 chromosomal genes related to genes involved in the formation of type IV pili in other gram-negative bacteria. However, E. coli K-12 does not produce type IV pili when grown under standard laboratory conditions. The results of reverse transcription-PCR, operon fusion analysis, and immunoblotting demonstrated that several of the putative E. coli piliation genes are expressed at very low levels. Increasing the level of expression of the major pilin gene ( ppdD ) and the linked assembly genes hofB and hofC (homologues of the Pseudomonas aeruginosa type IV pilus assembly genes pilB and pilC ) did not lead to pilus production. However, expression of the ppdD gene in P. aeruginosa led to assembly of PpdD into pili that were recognized by antibodies directed against the PpdD protein. Assembly of PpdD into pili in P. aeruginosa was dependent on the expression of the pilB and pilC genes and independent of expression of the P. aeruginosa pilin structural gene pilA.

Bibliography

Sauvonnet, N., Gounon, P., & Pugsley, A. P. (2000). PpdD Type IV Pilin of Escherichia coli K-12 Can Be Assembled into Pili in Pseudomonas aeruginosa. Journal of Bacteriology, 182(3), 848â854.

Authors 3

Nathalie Sauvonnet (first)
Pierre Gounon (additional)
Anthony P. Pugsley (additional)

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Dates

Type	When
Created	23 years, 1 month ago (July 27, 2002, 6:01 a.m.)
Deposited	4 years, 1 month ago (July 29, 2021, 1:56 p.m.)
Indexed	2 months, 1 week ago (June 27, 2025, 5:50 a.m.)
Issued	25 years, 7 months ago (Feb. 1, 2000)
Published	25 years, 7 months ago (Feb. 1, 2000)
Published Print	25 years, 7 months ago (Feb. 1, 2000)

Funders 0

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BibTeX

@article{Sauvonnet_2000, title={PpdD Type IV Pilin of Escherichia coli K-12 Can Be Assembled into Pili in Pseudomonas aeruginosa}, volume={182}, ISSN={1098-5530}, url={http://dx.doi.org/10.1128/jb.182.3.848-854.2000}, DOI={10.1128/jb.182.3.848-854.2000}, number={3}, journal={Journal of Bacteriology}, publisher={American Society for Microbiology}, author={Sauvonnet, Nathalie and Gounon, Pierre and Pugsley, Anthony P.}, year={2000}, month=feb, pages={848–854} }

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