DOI: 10.1128/jb.182.19.5521-5529.2000. Characterization of ssfR and ssgA , Two Genes Involved in Sporulation of Streptomyces griseus

Characterization of ssfR and ssgA , Two Genes Involved in Sporulation of Streptomyces griseus

10.1128/jb.182.19.5521-5529.2000

Crossref journal-article

American Society for Microbiology

Journal of Bacteriology (235)

Abstract

ABSTRACT In the presence of cefoxitin, which inhibits septum formation during sporulation, Streptomyces griseus is unable to sporulate, retaining the sonication sensitivity of nonsporulating hyphae. Cefoxitin- and sonication-resistant mutant SKK2600 was isolated and showed many morphological differences from its parental strain. A 3.6-kb DNA fragment that complemented the mutations of SKK2600 contained two open reading frames (ORFs), either of which could complement SKK2600. One ORF, designated ssfR , encoded a protein containing a potential DNA-binding helix-turn-helix motif close to its N terminus. SsfR is similar to members of a large family of transcriptional regulators, particularly IclR of Escherichia coli . The second ORF was identified as ssgA , a previously described sporulation gene from S. griseus (S. Kawamoto and J. C. Ensign, Actinomycetology 9:136–151, 1995). A point mutation of C to T seven nucleotides upstream of the UGA stop codon of ssfR was responsible for the phenotype of isolated mutant strain SKK2600. Surprisingly, this mutation should not change the primary structure of SsfR. The ssfR and ssgA disruption mutants were constructed and showed the “white” mutant phenotype, with some growth medium dependence. In addition, the ssfR null mutant sporulated ectopically in phosphate starvation medium.

Bibliography

Jiang, H., & Kendrick, K. E. (2000). Characterization of ssfR and ssgA , Two Genes Involved in Sporulation of Streptomyces griseus. Journal of Bacteriology, 182(19), 5521â5529.

Authors 2

Hao Jiang (first)
Kathleen E. Kendrick (additional)

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Dates

Type	When
Created	23 years, 1 month ago (July 27, 2002, 6 a.m.)
Deposited	4 years, 1 month ago (July 29, 2021, 1:54 p.m.)
Indexed	1 year ago (Aug. 9, 2024, 8:56 p.m.)
Issued	24 years, 11 months ago (Oct. 1, 2000)
Published	24 years, 11 months ago (Oct. 1, 2000)
Published Print	24 years, 11 months ago (Oct. 1, 2000)

Funders 0

None

BibTeX

@article{Jiang_2000, title={Characterization of ssfR and ssgA , Two Genes Involved in Sporulation of Streptomyces griseus}, volume={182}, ISSN={1098-5530}, url={http://dx.doi.org/10.1128/jb.182.19.5521-5529.2000}, DOI={10.1128/jb.182.19.5521-5529.2000}, number={19}, journal={Journal of Bacteriology}, publisher={American Society for Microbiology}, author={Jiang, Hao and Kendrick, Kathleen E.}, year={2000}, month=oct, pages={5521–5529} }

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