A PhoP-Regulated Outer Membrane Protease of Salmonella enterica Serovar Typhimurium Promotes Resistance to Alpha-Helical Antimicrobial Peptides
10.1128/jb.182.14.4077-4086.2000
Crossref journal-article
American Society for Microbiology
Journal of Bacteriology (235)
Abstract

ABSTRACT The outer membrane protein contents of Salmonella enterica serovar Typhimurium strains with PhoP/PhoQ regulon mutations were compared by two-dimensional gel electrophoresis. At least 26 species of outer membrane proteins (OMPs) were identified as being regulated by PhoP/PhoQ activation. One PhoP/PhoQ-activated OMP was identified by semiautomated tandem mass spectrometry coupled with electronic database searching as PgtE, a member of the Escherichia coli OmpT and Yersinia pestis Pla family of outer membrane proteases. Salmonella PgtE expression promoted resistance to alpha-helical cationic antimicrobial peptides (α-CAMPs). Strains expressing PgtE cleaved C18G, an 18-residue α-CAMP present in culture medium, indicating that protease activity is likely to be the mechanism of OmpT-mediated resistance to α-CAMPs. PhoP/PhoQ did not regulate the transcription or export of PgtE, indicating that another PhoP/PhoQ-dependent mechanism is required for PgtE outer membrane localization. PgtE is a posttranscriptionally regulated component of the PhoP/PhoQ regulon that contributes to Salmonella resistance to innate immunity.

Bibliography

Guina, T., Yi, E. C., Wang, H., Hackett, M., & Miller, S. I. (2000). A PhoP-Regulated Outer Membrane Protease of Salmonella enterica Serovar Typhimurium Promotes Resistance to Alpha-Helical Antimicrobial Peptides. Journal of Bacteriology, 182(14), 4077–4086.

Authors 5
  1. Tina Guina (first)
  2. Eugene C. Yi (additional)
  3. Houle Wang (additional)
  4. Murray Hackett (additional)
  5. Samuel I. Miller (additional)
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Dates
Type When
Created 23 years, 1 month ago (July 27, 2002, 6:03 a.m.)
Deposited 4 years, 1 month ago (July 29, 2021, 1:53 p.m.)
Indexed 1 week, 4 days ago (Aug. 19, 2025, 6:25 a.m.)
Issued 25 years, 1 month ago (July 15, 2000)
Published 25 years, 1 month ago (July 15, 2000)
Published Print 25 years, 1 month ago (July 15, 2000)
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@article{Guina_2000, title={A PhoP-Regulated Outer Membrane Protease of Salmonella enterica Serovar Typhimurium Promotes Resistance to Alpha-Helical Antimicrobial Peptides}, volume={182}, ISSN={1098-5530}, url={http://dx.doi.org/10.1128/jb.182.14.4077-4086.2000}, DOI={10.1128/jb.182.14.4077-4086.2000}, number={14}, journal={Journal of Bacteriology}, publisher={American Society for Microbiology}, author={Guina, Tina and Yi, Eugene C. and Wang, Houle and Hackett, Murray and Miller, Samuel I.}, year={2000}, month=jul, pages={4077–4086} }