DOI: 10.1128/jb.182.11.3183-3190.2000. <i>Yersinia enterocolitica</i> TyeA, an Intracellular Regulator of the Type III Machinery, Is Required for Specific Targeting of YopE, YopH, YopM, and YopN into the Cytosol of Eukaryotic Cells

Yersinia enterocolitica TyeA, an Intracellular Regulator of the Type III Machinery, Is Required for Specific Targeting of YopE, YopH, YopM, and YopN into the Cytosol of Eukaryotic Cells

10.1128/jb.182.11.3183-3190.2000

Crossref journal-article

American Society for Microbiology

Journal of Bacteriology (235)

Abstract

ABSTRACT Pathogenic Yersinia species employ type III machines to target effector Yops into the cytosol of eukaryotic cells. Yersinia tyeA mutants are thought to be defective in the targeting of YopE and YopH without affecting the injection of YopM, YopN, YopO, YopP, and YopT into the cytosol of eukaryotic cells. One model suggests that TyeA may form a tether between YopN (LcrE) and YopD on the bacterial surface, a structure that may translocate YopE and YopH across the plasma membrane of eukaryotic cells (M. Iriarte, M. P. Sory, A. Boland, A. P. Boyd, S. D. Mills, I. Lambermont, and G. R. Cornelis, EMBO J. 17:1907–1918, 1998). We have examined the injection of Yop proteins by tyeA mutant yersiniae with the digitonin fractionation technique. We find that tyeA mutant yersiniae not only secreted YopE, YopH, YopM, and YopN into the extracellular medium but also targeted these polypeptides into the cytosol of HeLa cells. Protease protection, cell fractionation, and affinity purification experiments suggest that TyeA is located intracellularly and binds to YopN or YopD. We propose a model whereby TyeA functions as a negative regulator of the type III targeting pathway in the cytoplasm of yersiniae, presumably by preventing the export of YopN.

Bibliography

Cheng, L. W., & Schneewind, O. (2000). Yersinia enterocolitica TyeA, an Intracellular Regulator of the Type III Machinery, Is Required for Specific Targeting of YopE, YopH, YopM, and YopN into the Cytosol of Eukaryotic Cells. Journal of Bacteriology, 182(11), 3183â3190.

Authors 2

Luisa W. Cheng (first)
Olaf Schneewind (additional)

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Dates

Type	When
Created	23 years, 1 month ago (July 27, 2002, 6:03 a.m.)
Deposited	4 years, 1 month ago (July 29, 2021, 1:52 p.m.)
Indexed	2 months, 3 weeks ago (June 4, 2025, 6:29 a.m.)
Issued	25 years, 2 months ago (June 1, 2000)
Published	25 years, 2 months ago (June 1, 2000)
Published Print	25 years, 2 months ago (June 1, 2000)

Funders 0

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BibTeX

@article{Cheng_2000, title={Yersinia enterocolitica TyeA, an Intracellular Regulator of the Type III Machinery, Is Required for Specific Targeting of YopE, YopH, YopM, and YopN into the Cytosol of Eukaryotic Cells}, volume={182}, ISSN={1098-5530}, url={http://dx.doi.org/10.1128/jb.182.11.3183-3190.2000}, DOI={10.1128/jb.182.11.3183-3190.2000}, number={11}, journal={Journal of Bacteriology}, publisher={American Society for Microbiology}, author={Cheng, Luisa W. and Schneewind, Olaf}, year={2000}, month=jun, pages={3183–3190} }

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