DOI: 10.1128/jb.182.10.2771-2777.2000. The <i>Bacillus subtilis</i> GTP Binding Protein Obg and Regulators of the ς <sup>B</sup> Stress Response Transcription Factor Cofractionate with Ribosomes

The Bacillus subtilis GTP Binding Protein Obg and Regulators of the ς B Stress Response Transcription Factor Cofractionate with Ribosomes

10.1128/jb.182.10.2771-2777.2000

Crossref journal-article

American Society for Microbiology

Journal of Bacteriology (235)

Abstract

ABSTRACT Obg, an essential GTP binding protein of Bacillus subtilis , is necessary for stress activation of the ς B transcription factor. We investigated Obg's cellular associations by differential centrifugation of crude B. subtilis extracts, using an anti-Obg antibody as a probe to monitor Obg during the fractionation, and by fluorescent microscopy of a B. subtilis strain in which Obg was fused to green fluorescent protein. The results indicated that Obg is part of a large cytoplasmic complex. In subsequent analyses, Obg coeluted with ribosomal subunits during gel filtration of B. subtilis lysates on Sephacryl S-400 and specifically bound to ribosomal protein L13 in an affinity blot assay. Probing the gel filtration fractions with antibodies specific for ς B and its coexpressed regulators (Rsb proteins) revealed coincident elution of the upstream components of the ς B stress activation pathway (RsbR, -S, and -T) with Obg and the ribosomal subunits. The data implicate ribosome function as a possible mediator of the activity of Obg and the stress induction of ς B .

Bibliography

Scott, J. M., Ju, J., Mitchell, T., & Haldenwang, W. G. (2000). The Bacillus subtilis GTP Binding Protein Obg and Regulators of the Ï B Stress Response Transcription Factor Cofractionate with Ribosomes. Journal of Bacteriology, 182(10), 2771â2777.

Authors 4

Janelle M. Scott (first)
Jingliang Ju (additional)
Theresa Mitchell (additional)
W. G. Haldenwang (additional)

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Dates

Type	When
Created	23 years, 1 month ago (July 27, 2002, 6 a.m.)
Deposited	4 years, 1 month ago (July 29, 2021, 1:52 p.m.)
Indexed	2 months ago (July 2, 2025, 2:31 p.m.)
Issued	25 years, 3 months ago (May 15, 2000)
Published	25 years, 3 months ago (May 15, 2000)
Published Print	25 years, 3 months ago (May 15, 2000)

Funders 0

None

BibTeX

@article{Scott_2000, title={The Bacillus subtilis GTP Binding Protein Obg and Regulators of the ς B Stress Response Transcription Factor Cofractionate with Ribosomes}, volume={182}, ISSN={1098-5530}, url={http://dx.doi.org/10.1128/jb.182.10.2771-2777.2000}, DOI={10.1128/jb.182.10.2771-2777.2000}, number={10}, journal={Journal of Bacteriology}, publisher={American Society for Microbiology}, author={Scott, Janelle M. and Ju, Jingliang and Mitchell, Theresa and Haldenwang, W. G.}, year={2000}, month=may, pages={2771–2777} }

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