DOI: 10.1128/jb.181.4.1356-1359.1999. Cell Surface Galactosylation Is Essential for Nonsexual Flocculation in <i>Schizosaccharomyces pombe</i>

Cell Surface Galactosylation Is Essential for Nonsexual Flocculation in Schizosaccharomyces pombe

10.1128/jb.181.4.1356-1359.1999

Crossref journal-article

American Society for Microbiology

Journal of Bacteriology (235)

Abstract

ABSTRACT We have isolated fission yeast mutants that constitutively flocculate upon growth in liquid media. One of these mutants, the gsf1 mutant, was found to cause dominant, nonsexual, and calcium-dependent aggregation of cells into flocs. Its flocculation was inhibited by the addition of galactose but was not affected by the addition of mannose or glucose, unlike Saccharomyces cerevisiae FLO mutants. The gsf1 mutant coflocculated with Schizosaccharomyces pombe wild-type cells, while no coflocculation was found with galactose-deficient ( gms1 Δ) cells. Moreover, flocculation of the gsf1 mutant was also inhibited by addition of cell wall galactomannan from wild-type cells but not from gms1 Δ cells. These results suggested that galactose residues in the cell wall glycoproteins may be receptors of gsf1 -mediated flocculation, and therefore cell surface galactosylation is required for nonsexual flocculation in S. pombe .

Bibliography

Tanaka, N., Awai, A., Bhuiyan, M. S. A., Fujita, K., Fukui, H., & Takegawa, K. (1999). Cell Surface Galactosylation Is Essential for Nonsexual Flocculation in Schizosaccharomyces pombe. Journal of Bacteriology, 181(4), 1356â1359.

Authors 6

Naotaka Tanaka (first)
Atsuro Awai (additional)
M. Shah Alam Bhuiyan (additional)
Kiyotaka Fujita (additional)
Hiroshi Fukui (additional)
Kaoru Takegawa (additional)

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Dates

Type	When
Created	5 years, 8 months ago (Dec. 31, 2019, 11:45 a.m.)
Deposited	4 years, 1 month ago (July 29, 2021, 1:51 p.m.)
Indexed	1 year, 6 months ago (March 5, 2024, 3:21 p.m.)
Issued	26 years, 6 months ago (Feb. 15, 1999)
Published	26 years, 6 months ago (Feb. 15, 1999)
Published Print	26 years, 6 months ago (Feb. 15, 1999)

Funders 0

None

BibTeX

@article{Tanaka_1999, title={Cell Surface Galactosylation Is Essential for Nonsexual Flocculation in Schizosaccharomyces pombe}, volume={181}, ISSN={1098-5530}, url={http://dx.doi.org/10.1128/jb.181.4.1356-1359.1999}, DOI={10.1128/jb.181.4.1356-1359.1999}, number={4}, journal={Journal of Bacteriology}, publisher={American Society for Microbiology}, author={Tanaka, Naotaka and Awai, Atsuro and Bhuiyan, M. Shah Alam and Fujita, Kiyotaka and Fukui, Hiroshi and Takegawa, Kaoru}, year={1999}, month=feb, pages={1356–1359} }

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