DOI: 10.1128/jb.181.2.670-674.1999. Isolation and Characterization of the <i>nikR</i> Gene Encoding a Nickel-Responsive Regulator in <i>Escherichia coli</i>

Isolation and Characterization of the nikR Gene Encoding a Nickel-Responsive Regulator in Escherichia coli

10.1128/jb.181.2.670-674.1999

Crossref journal-article

American Society for Microbiology

Journal of Bacteriology (235)

Abstract

ABSTRACT Expression of the nickel-specific transport system encoded by the Escherichia coli nikABCDE operon is repressed by a high concentration of nickel. By using random transposon Tn 10 insertion, we isolated mutants in which expression of the nik operon became constitutive with respect to nickel. We have identified the corresponding nikR gene which encodes a nickel-responsive regulator. Expression of nikR was partially controlled by Fnr through transcription from the nikA promoter region. In addition, a specific transcription start site for the constitutive expression of nikR was found 51 bp upstream of the nikR gene.

Bibliography

De Pina, K., Desjardin, V., Mandrand-Berthelot, M.-A., Giordano, G., & Wu, L.-F. (1999). Isolation and Characterization of the nikR Gene Encoding a Nickel-Responsive Regulator in Escherichia coli. Journal of Bacteriology, 181(2), 670â674.

Authors 5

Karinne De Pina (first)
Valerie Desjardin (additional)
Marie-Andree Mandrand-Berthelot (additional)
Gerard Giordano (additional)
Long-Fei Wu (additional)

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Dates

Type	When
Created	5 years, 8 months ago (Dec. 31, 2019, 11:41 a.m.)
Deposited	4 years, 1 month ago (July 29, 2021, 1:50 p.m.)
Indexed	1 month, 1 week ago (July 30, 2025, 10:22 a.m.)
Issued	26 years, 7 months ago (Jan. 15, 1999)
Published	26 years, 7 months ago (Jan. 15, 1999)
Published Print	26 years, 7 months ago (Jan. 15, 1999)

Funders 0

None

BibTeX

@article{De_Pina_1999, title={Isolation and Characterization of the nikR Gene Encoding a Nickel-Responsive Regulator in Escherichia coli}, volume={181}, ISSN={1098-5530}, url={http://dx.doi.org/10.1128/jb.181.2.670-674.1999}, DOI={10.1128/jb.181.2.670-674.1999}, number={2}, journal={Journal of Bacteriology}, publisher={American Society for Microbiology}, author={De Pina, Karinne and Desjardin, Valerie and Mandrand-Berthelot, Marie-Andree and Giordano, Gerard and Wu, Long-Fei}, year={1999}, month=jan, pages={670–674} }

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