DOI: 10.1128/jb.181.10.3096-3104.1999. A HilA-Independent Pathway to <i>Salmonella typhimurium</i> Invasion Gene Transcription

A HilA-Independent Pathway to Salmonella typhimurium Invasion Gene Transcription

10.1128/jb.181.10.3096-3104.1999

Crossref journal-article

American Society for Microbiology

Journal of Bacteriology (235)

Abstract

ABSTRACT Salmonella typhimurium invasion of nonphagocytic cells requires the expression of a type III secretion system (TTSS) encoded within Salmonella pathogenicity island 1 (SPI1). TTSS gene transcription is activated in response to environmental signals and requires transcriptional regulators encoded within (HilA) and outside (SirA) SPI1. Two unique loci, sirB and sirC , which contribute to SPI1 gene transcription were defined. sirC is an SPI1-encoded transcription factor of the AraC family that contributes to the invasive phenotype. sirB is required for maximal expression of sirC and consists of two open reading frames located near kdsA , a gene involved in lipopolysaccharide biosynthesis. sirC expression, unlike expression of other SPI1 genes, does not require HilA. Overexpression of sirC or sirA restores expression of a subset of SPI1 genes, including invF and sspC , in the absence of HilA. These data define roles for SirC and SirA as part of a HilA-independent pathway to SPI1 gene expression. We postulate that HilA-independent activation of inv expression is important for efficient assembly and function of the SPI1 TTSS.

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Rakeman, J. L., Bonifield, H. R., & Miller, S. I. (1999). A HilA-Independent Pathway to Salmonella typhimurium Invasion Gene Transcription. Journal of Bacteriology, 181(10), 3096â3104.

Authors 3

Jennifer L. Rakeman (first)
Heather R. Bonifield (additional)
Samuel I. Miller (additional)

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Dates

Type	When
Created	5 years, 8 months ago (Dec. 31, 2019, 11:34 a.m.)
Deposited	4 years, 1 month ago (July 29, 2021, 1:46 p.m.)
Indexed	3 months, 1 week ago (May 28, 2025, 5:49 a.m.)
Issued	26 years, 3 months ago (May 15, 1999)
Published	26 years, 3 months ago (May 15, 1999)
Published Print	26 years, 3 months ago (May 15, 1999)

Funders 0

None

BibTeX

@article{Rakeman_1999, title={A HilA-Independent Pathway to Salmonella typhimurium Invasion Gene Transcription}, volume={181}, ISSN={1098-5530}, url={http://dx.doi.org/10.1128/jb.181.10.3096-3104.1999}, DOI={10.1128/jb.181.10.3096-3104.1999}, number={10}, journal={Journal of Bacteriology}, publisher={American Society for Microbiology}, author={Rakeman, Jennifer L. and Bonifield, Heather R. and Miller, Samuel I.}, year={1999}, month=may, pages={3096–3104} }

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  "abstract": "<jats:title>ABSTRACT</jats:title>\n          <jats:p>\n            <jats:italic>Salmonella typhimurium</jats:italic>\n            invasion of nonphagocytic cells requires the expression of a type III secretion system (TTSS) encoded within\n            <jats:italic>Salmonella</jats:italic>\n            pathogenicity island 1 (SPI1). TTSS gene transcription is activated in response to environmental signals and requires transcriptional regulators encoded within (HilA) and outside (SirA) SPI1. Two unique loci,\n            <jats:italic>sirB</jats:italic>\n            and\n            <jats:italic>sirC</jats:italic>\n            , which contribute to SPI1 gene transcription were defined.\n            <jats:italic>sirC</jats:italic>\n            is an SPI1-encoded transcription factor of the AraC family that contributes to the invasive phenotype.\n            <jats:italic>sirB</jats:italic>\n            is required for maximal expression of\n            <jats:italic>sirC</jats:italic>\n            and consists of two open reading frames located near\n            <jats:italic>kdsA</jats:italic>\n            , a gene involved in lipopolysaccharide biosynthesis.\n            <jats:italic>sirC</jats:italic>\n            expression, unlike expression of other SPI1 genes, does not require HilA. Overexpression of\n            <jats:italic>sirC</jats:italic>\n            or\n            <jats:italic>sirA</jats:italic>\n            restores expression of a subset of SPI1 genes, including\n            <jats:italic>invF</jats:italic>\n            and\n            <jats:italic>sspC</jats:italic>\n            , in the absence of HilA. These data define roles for SirC and SirA as part of a HilA-independent pathway to SPI1 gene expression. We postulate that HilA-independent activation of\n            <jats:italic>inv</jats:italic>\n            expression is important for efficient assembly and function of the SPI1 TTSS.\n          </jats:p>",
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