Helicobacter pylori porCDABandoorDABCGenes Encode Distinct Pyruvate:Flavodoxin and 2-Oxoglutarate:Acceptor Oxidoreductases Which Mediate Electron Transport to NADP
10.1128/jb.180.5.1119-1128.1998
Crossref journal-article
American Society for Microbiology
Journal of Bacteriology (235)
Abstract

ABSTRACTHelicobacter pylori, a major cause of human gastric disease, is a microaerophilic bacterium that contains neither pyruvate nor 2-oxoglutarate dehydrogenase activity. Previous studies (N. J. Hughes, P. A. Chalk, C. L. Clayton, and D. J. Kelly, J. Bacteriol. 177:3953–3959, 1995) have indicated that the major routes for the generation of acetyl coenzyme A (acetyl-CoA) and succinyl-CoA are via pyruvate:flavodoxin oxidoreductase (POR) and 2-oxoglutarate:acceptor oxidoreductase (OOR), respectively. The purified POR is a heterotetrameric protein, with subunits of 48 (PorA), 36 (PorB), 24 (PorC), and 14 (PorD) kDa. In this study OOR has been purified, and it is similarly composed of polypeptides of 43 (OorA), 33 (OorB), 24 (OorC), and 10 (OorD) kDa. Both POR and OOR are oxygen labile and are likely to be major contributors to the microaerophilic phenotype ofH. pylori. Unlike POR, OOR was unable to use a previously identified flavodoxin (FldA) as an electron acceptor. Although the purified enzymes were unable to reduce NAD(P), electrons from both pyruvate and 2-oxoglutarate could reduce NADP in cell extracts, consistent with a role for these oxidoreductases in the provision of NADPH as a respiratory electron donor. TheH. pylori por,oor, andfldAgenes were cloned and sequenced. The deducedporgene products showed significant sequence similarity to archaeal four-subunit 2-oxoacid:acceptor oxidoreductases. However, the amino acid sequences of OorA and -B were more closely related to that of the two-subunit POR of the aerobic halophileHalobacterium halobium. BothporDandoorDencode integral ferredoxin-like subunits. POR and OOR are probably essential enzymes inH. pylori, as insertion inactivation ofporBandoorAappeared to be lethal.

Bibliography

Hughes, N. J., Clayton, C. L., Chalk, P. A., & Kelly, D. J. (1998). Helicobacter pylori porCDABandoorDABCGenes Encode Distinct Pyruvate:Flavodoxin and 2-Oxoglutarate:Acceptor Oxidoreductases Which Mediate Electron Transport to NADP. Journal of Bacteriology, 180(5), 1119–1128.

Authors 4
  1. Nicky J. Hughes (first)
  2. Chris L. Clayton (additional)
  3. Peter A. Chalk (additional)
  4. David J. Kelly (additional)
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Dates
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Created 5 years, 8 months ago (Dec. 31, 2019, 11:30 a.m.)
Deposited 2 years, 10 months ago (Oct. 9, 2022, 12:21 p.m.)
Indexed 1 month ago (Aug. 6, 2025, 9:09 a.m.)
Issued 27 years, 6 months ago (March 1, 1998)
Published 27 years, 6 months ago (March 1, 1998)
Published Print 27 years, 6 months ago (March 1, 1998)
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@article{Hughes_1998, title={Helicobacter pylori porCDABandoorDABCGenes Encode Distinct Pyruvate:Flavodoxin and 2-Oxoglutarate:Acceptor Oxidoreductases Which Mediate Electron Transport to NADP}, volume={180}, ISSN={1098-5530}, url={http://dx.doi.org/10.1128/jb.180.5.1119-1128.1998}, DOI={10.1128/jb.180.5.1119-1128.1998}, number={5}, journal={Journal of Bacteriology}, publisher={American Society for Microbiology}, author={Hughes, Nicky J. and Clayton, Chris L. and Chalk, Peter A. and Kelly, David J.}, year={1998}, month=mar, pages={1119–1128} }