DOI: 10.1128/jb.180.23.6107-6116.1998. Localization of Cell Division Protein FtsQ by Immunofluorescence Microscopy in Dividing and Nondividing Cells of <i>Escherichia coli</i>

Localization of Cell Division Protein FtsQ by Immunofluorescence Microscopy in Dividing and Nondividing Cells of Escherichia coli

10.1128/jb.180.23.6107-6116.1998

Crossref journal-article

American Society for Microbiology

Journal of Bacteriology (235)

Abstract

ABSTRACT The localization of cell division protein FtsQ in Escherichia coli wild-type cells was studied by immunofluorescence microscopy with specific monoclonal antibodies. FtsQ could be localized to the division site in constricting cells. FtsQ could also localize to the division site in ftsQ1 (Ts) cells grown at the permissive temperature. A hybrid protein in which the cytoplasmic domain and the transmembrane domain were derived from the γ form of penicillin-binding protein 1B and the periplasmic domain was derived from FtsQ was also able to localize to the division site. This result indicates that the periplasmic domain of FtsQ determines the localization of FtsQ, as has also been concluded by others for the periplasmic domain of FtsN. Noncentral FtsQ foci were found in the area of the cell where the nucleoid resides and were therefore assumed to represent sites where the FtsQ protein is synthesized and simultaneously inserted into the cytoplasmic membrane.

Bibliography

Buddelmeijer, N., Aarsman, M. E. G., Kolk, A. H. J., Vicente, M., & Nanninga, N. (1998). Localization of Cell Division Protein FtsQ by Immunofluorescence Microscopy in Dividing and Nondividing Cells of Escherichia coli. Journal of Bacteriology, 180(23), 6107â6116.

Authors 5

Nienke Buddelmeijer (first)
Mirjam E. G. Aarsman (additional)
Arend H. J. Kolk (additional)
Miguel Vicente (additional)
Nanne Nanninga (additional)

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Dates

Type	When
Created	5 years, 5 months ago (March 9, 2020, 3:46 p.m.)
Deposited	4 years, 1 month ago (July 29, 2021, 2:24 p.m.)
Indexed	3 weeks, 2 days ago (Aug. 6, 2025, 8 a.m.)
Issued	26 years, 8 months ago (Dec. 1, 1998)
Published	26 years, 8 months ago (Dec. 1, 1998)
Published Print	26 years, 8 months ago (Dec. 1, 1998)

Funders 0

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BibTeX

@article{Buddelmeijer_1998, title={Localization of Cell Division Protein FtsQ by Immunofluorescence Microscopy in Dividing and Nondividing Cells of Escherichia coli}, volume={180}, ISSN={1098-5530}, url={http://dx.doi.org/10.1128/jb.180.23.6107-6116.1998}, DOI={10.1128/jb.180.23.6107-6116.1998}, number={23}, journal={Journal of Bacteriology}, publisher={American Society for Microbiology}, author={Buddelmeijer, Nienke and Aarsman, Mirjam E. G. and Kolk, Arend H. J. and Vicente, Miguel and Nanninga, Nanne}, year={1998}, month=dec, pages={6107–6116} }

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