DOI: 10.1128/jb.180.19.5240-5242.1998. Regulation of <i>Escherichia coli secA</i> by Cellular Protein Secretion Proficiency Requires an Intact Gene X Signal Sequence and an Active Translocon

Regulation of Escherichia coli secA by Cellular Protein Secretion Proficiency Requires an Intact Gene X Signal Sequence and an Active Translocon

10.1128/jb.180.19.5240-5242.1998

Crossref journal-article

American Society for Microbiology

Journal of Bacteriology (235)

Abstract

ABSTRACT secA is translationally regulated by the protein secretion proficiency state of the Escherichia coli cell. This regulation was explored by making signal sequence mutations in the gene upstream of secA , gene X, which promotes secA translational coupling. Gene X signal sequence mutants were constitutive for secA expression, while prlA alleles partially restored secA regulation. These results show that interaction of the pre-gene X protein with the translocon is required for proper secA regulation. Furthermore, gene X signal sequence mutations disrupted secA regulation only in the cis configuration. We propose that nascent pre-gene X protein interacts with the translocon during its secretion to constitute the secretion sensor.

Bibliography

Oliver, D., Norman, J., & Sarker, S. (1998). Regulation of Escherichia coli secA by Cellular Protein Secretion Proficiency Requires an Intact Gene X Signal Sequence and an Active Translocon. Journal of Bacteriology, 180(19), 5240â5242.

Authors 3

Donald Oliver (first)
Jessica Norman (additional)
Shameema Sarker (additional)

References 28 Referenced 50

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Dates

Type	When
Created	5 years, 8 months ago (Dec. 31, 2019, 11:27 a.m.)
Deposited	4 years, 1 month ago (July 29, 2021, 2:24 p.m.)
Indexed	1 year ago (Aug. 5, 2024, 12:39 a.m.)
Issued	26 years, 11 months ago (Oct. 1, 1998)
Published	26 years, 11 months ago (Oct. 1, 1998)
Published Print	26 years, 11 months ago (Oct. 1, 1998)

Funders 0

None

BibTeX

@article{Oliver_1998, title={Regulation of Escherichia coli secA by Cellular Protein Secretion Proficiency Requires an Intact Gene X Signal Sequence and an Active Translocon}, volume={180}, ISSN={1098-5530}, url={http://dx.doi.org/10.1128/jb.180.19.5240-5242.1998}, DOI={10.1128/jb.180.19.5240-5242.1998}, number={19}, journal={Journal of Bacteriology}, publisher={American Society for Microbiology}, author={Oliver, Donald and Norman, Jessica and Sarker, Shameema}, year={1998}, month=oct, pages={5240–5242} }

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