DOI: 10.1128/jb.180.16.4177-4183.1998. A Mutation in a Purported Regulatory Gene Affects Control of Sterol Uptake in <i>Saccharomyces cerevisiae</i>

A Mutation in a Purported Regulatory Gene Affects Control of Sterol Uptake in Saccharomyces cerevisiae

10.1128/jb.180.16.4177-4183.1998

Crossref journal-article

American Society for Microbiology

Journal of Bacteriology (235)

Abstract

ABSTRACT Aerobically growing wild-type strains of Saccharomyces cerevisiae are unable to take exogenously supplied sterols from media. This aerobic sterol exclusion is vitiated under anaerobic conditions, in heme-deficient strains, and under some conditions of impaired sterol synthesis. Mutants which can take up sterols aerobically in heme-competent cells have been selected. One of these mutations, designated upc2-1 , gives a pleiotropic phenotype in characteristics as diverse as aerobic accumulation of sterols, total lipid storage, sensitivity to metabolic inhibitors, response to altered sterol structures, and cation requirements. During experiments designed to ascertain the effects of various cations on yeast with sterol alterations, it was observed that upc2-1 was hypersensitive to Ca 2+ . Using resistance to Ca 2+ as a screening vehicle, we cloned UPC2 and showed that it is YDR213W, an open reading frame on chromosome IV. This belongs to a fungal regulatory family containing the Zn(II)2Cys6 binuclear cluster DNA binding domain. The single guanine-to-adenine transition in upc2-1 gives a predicted amino acid change from glycine to aspartic acid. The regulatory defect explains the semidominance and pleiotropic effects of upc2-1.

Bibliography

Crowley, J. H., Leak, F. W., Shianna, K. V., Tove, S., & Parks, L. W. (1998). A Mutation in a Purported Regulatory Gene Affects Control of Sterol Uptake in Saccharomyces cerevisiae. Journal of Bacteriology, 180(16), 4177â4183.

Authors 5

James H. Crowley (first)
Frank W. Leak (additional)
Kevin V. Shianna (additional)
Shirley Tove (additional)
Leo W. Parks (additional)

References 24 Referenced 100

Berben G. Dumont J. Gilliquet V. Bolle P. A. Hilger F. The YDp plasmids: a uniform set of vectors bearing versatile gene disruption cassettes for Saccharomyces cerevisiae.Yeast71991475477 (10.1002/yea.320070506) / Yeast / The YDp plasmids: a uniform set of vectors bearing versatile gene disruption cassettes for Saccharomyces cerevisiae by Berben G. (1991)
Bourot S. Karst F. Isolation and characterization of the Saccharomyces cerevisiae SUT1 gene involved in sterol uptake.Gene165199597102 (10.1016/0378-1119(95)00478-O) / Gene / Isolation and characterization of the Saccharomyces cerevisiae SUT1 gene involved in sterol uptake by Bourot S. (1995)
Eberhardt I. Hohmann S. Strategy for deletion of complete open reading frames in Saccharomyces cerevisiae.Curr. Genet.271995306308 (10.1007/BF00352097) / Curr. Genet. / Strategy for deletion of complete open reading frames in Saccharomyces cerevisiae by Eberhardt I. (1995)
10.1093/nar/20.6.1425
Gollub E. G. Liu K. P. Dayan J. Adelsberg M. Sprinson D. B. Yeast mutations deficient in heme biosynthesis and a heme mutant additionally blocked in cyclization of 2,3 oxidosqualene.J. Biol. Chem.252197728462854 (10.1016/S0021-9258(17)40440-6) / J. Biol. Chem. / Yeast mutations deficient in heme biosynthesis and a heme mutant additionally blocked in cyclization of 2,3 oxidosqualene by Gollub E. G. (1977)
Keesler G. A. Casey W. M. Parks L. W. Stimulation by heme of steryl ester synthase and aerobic sterol exclusion in the yeast Saccharomyces cerevisiae.Arch. Biochem. Biophys.2961992474481 (10.1016/0003-9861(92)90600-2) / Arch. Biochem. Biophys. / Stimulation by heme of steryl ester synthase and aerobic sterol exclusion in the yeast Saccharomyces cerevisiae by Keesler G. A. (1992)
Leber R. Zinser E. Hrastnik C. Paltauf F. Daum G. Export of steryl esters from lipid particles and release of free sterols in the yeast, Saccharomyces cerevisiae.Biochim. Biophys. Acta12341995119126 (10.1016/0005-2736(94)00270-Y) / Biochim. Biophys. Acta / Export of steryl esters from lipid particles and release of free sterols in the yeast, Saccharomyces cerevisiae by Leber R. (1995)
Lewis T. L. Keesler G. A. Fenner G. P. Parks L. W. Pleiotropic mutations in Saccharomyces cerevisiae affecting sterol uptake and metabolism.Yeast4198893106 (10.1002/yea.320040203) / Yeast / Pleiotropic mutations in Saccharomyces cerevisiae affecting sterol uptake and metabolism by Lewis T. L. (1988)
10.1128/AAC.34.9.1660
Lorenz R. T. Parks L. W. Involvement of heme components in sterol metabolism of Saccharomyces cerevisiae.Lipids261991598603 (10.1007/BF02536423) / Lipids / Involvement of heme components in sterol metabolism of Saccharomyces cerevisiae by Lorenz R. T. (1991)
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Parks L. W. Unpublished results.
Parks L. W. Casey W. M. Physiological implications of sterol biosynthesis in yeast.Annu. Rev. Microbiol.49199595116 (10.1146/annurev.mi.49.100195.000523) / Annu. Rev. Microbiol. / Physiological implications of sterol biosynthesis in yeast by Parks L. W. (1995)
Rodriguez R. J. Low C. Bottema C. D. K. Parks L. W. Multiple functions for sterols in Saccharomyces cerevisiae.Biochim. Biophys. Acta8371985336343 (10.1016/0005-2760(85)90057-8) / Biochim. Biophys. Acta / Multiple functions for sterols in Saccharomyces cerevisiae by Rodriguez R. J. (1985)
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10.1128/mcb.11.5.2629-2640.1991

Dates

Type	When
Created	5 years, 7 months ago (Dec. 31, 2019, 11:25 a.m.)
Deposited	4 years, 1 month ago (July 29, 2021, 2:22 p.m.)
Indexed	1 year ago (Aug. 10, 2024, 1:55 a.m.)
Issued	27 years ago (Aug. 15, 1998)
Published	27 years ago (Aug. 15, 1998)
Published Print	27 years ago (Aug. 15, 1998)

Funders 0

None

BibTeX

@article{Crowley_1998, title={A Mutation in a Purported Regulatory Gene Affects Control of Sterol Uptake in Saccharomyces cerevisiae}, volume={180}, ISSN={1098-5530}, url={http://dx.doi.org/10.1128/jb.180.16.4177-4183.1998}, DOI={10.1128/jb.180.16.4177-4183.1998}, number={16}, journal={Journal of Bacteriology}, publisher={American Society for Microbiology}, author={Crowley, James H. and Leak, Frank W. and Shianna, Kevin V. and Tove, Shirley and Parks, Leo W.}, year={1998}, month=aug, pages={4177–4183} }

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    {
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    }
  ]
}