YscO of Yersinia pestis Is a Mobile Core Component of the Yop Secretion System
10.1128/jb.180.15.3882-3890.1998
Crossref journal-article
American Society for Microbiology
Journal of Bacteriology (235)
Abstract

ABSTRACT The Yersinia pestis low-Ca 2+ response stimulon is responsible for the temperature- and Ca 2+ -regulated expression and secretion of plasmid pCD1-encoded antihost proteins (V antigen and Yops). We have previously shown that lcrD , yscC , yscD , yscG , and yscR encode proteins that are essential for high-level expression and secretion of V antigen and Yops at 37°C in the absence of Ca 2+ . In this study, we characterized yscO of the Yop secretion ( ysc ) operon that contains yscN through yscU by determining the localization of its gene product and the phenotype of an in-frame deletion. The yscO mutant grew and expressed the same levels of Yops as the parent at 37°C in the presence of Ca 2+ . In the absence of Ca 2+ , the mutant grew independently of Ca 2+ , expressed only basal levels of V antigen and Yops, and failed to secrete these. These defects could be partially complemented by providing yscO in trans in the yscO mutant. Overexpression of YopM and V antigen in the mutant failed to restore the export of either protein, showing that the mutation had a direct effect on secretion. These results indicated that the yscO gene product is required for high-level expression and secretion of V antigen and Yops. YscO was found by immunoblot analysis in the soluble and membrane fractions of bacteria growing at 37°C irrespective of the presence of Ca 2+ and in the culture medium in the absence of Ca 2+ . YscO is the only mobile protein identified so far in the Yersinia species that is required for secretion of V antigen and Yops.

Bibliography

Payne, P. L., & Straley, S. C. (1998). YscO of Yersinia pestis Is a Mobile Core Component of the Yop Secretion System. Journal of Bacteriology, 180(15), 3882–3890.

Authors 2
  1. Patricia L. Payne (first)
  2. Susan C. Straley (additional)
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Dates
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Created 5 years, 7 months ago (Dec. 31, 2019, 11:25 a.m.)
Deposited 4 years ago (July 29, 2021, 2:23 p.m.)
Indexed 2 months, 2 weeks ago (June 4, 2025, 6:32 a.m.)
Issued 27 years ago (Aug. 1, 1998)
Published 27 years ago (Aug. 1, 1998)
Published Print 27 years ago (Aug. 1, 1998)
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@article{Payne_1998, title={YscO of Yersinia pestis Is a Mobile Core Component of the Yop Secretion System}, volume={180}, ISSN={1098-5530}, url={http://dx.doi.org/10.1128/jb.180.15.3882-3890.1998}, DOI={10.1128/jb.180.15.3882-3890.1998}, number={15}, journal={Journal of Bacteriology}, publisher={American Society for Microbiology}, author={Payne, Patricia L. and Straley, Susan C.}, year={1998}, month=aug, pages={3882–3890} }