DOI: 10.1128/jb.180.13.3441-3447.1998. Membrane-Bound Lytic Endotransglycosylase in <i>Escherichia coli</i>

Membrane-Bound Lytic Endotransglycosylase in Escherichia coli

10.1128/jb.180.13.3441-3447.1998

Crossref journal-article

American Society for Microbiology

Journal of Bacteriology (235)

Abstract

ABSTRACT The gene for a novel endotype membrane-bound lytic transglycosylase, emtA , was mapped at 26.7 min of the E. coli chromosome. EmtA is a lipoprotein with an apparent molecular mass of 22 kDa. Overexpression of the emtA gene did not result in bacteriolysis in vivo, but the enzyme was shown to hydrolyze glycan strands isolated from murein by amidase treatment. The formation of tetra- and hexasaccharides, but no disaccharides, reflects the endospecificity of the enzyme. The products are characterized by the presence of 1,6-anhydromuramic acid, indicating a lytic transglycosylase reaction mechanism. EmtA may function as a formatting enzyme that trims the nascent murein strands produced by the murein synthesis machinery into proper sizes, or it may be involved in the formation of tightly controlled minor holes in the murein sacculus to facilitate the export of bulky compounds across the murein barrier.

Bibliography

Kraft, A. R., Templin, M. F., & HoÌltje, J.-V. (1998). Membrane-Bound Lytic Endotransglycosylase in Escherichia coli. Journal of Bacteriology, 180(13), 3441â3447.

Authors 3

Angelika R. Kraft (first)
Markus F. Templin (additional)
Joachim-Volker Höltje (additional)

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Dates

Type	When
Created	5 years, 8 months ago (Dec. 31, 2019, 11:24 a.m.)
Deposited	4 years, 1 month ago (July 29, 2021, 2:22 p.m.)
Indexed	4 weeks ago (Aug. 3, 2025, 7:01 p.m.)
Issued	27 years, 2 months ago (July 1, 1998)
Published	27 years, 2 months ago (July 1, 1998)
Published Print	27 years, 2 months ago (July 1, 1998)

Funders 0

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BibTeX

@article{Kraft_1998, title={Membrane-Bound Lytic Endotransglycosylase in Escherichia coli}, volume={180}, ISSN={1098-5530}, url={http://dx.doi.org/10.1128/jb.180.13.3441-3447.1998}, DOI={10.1128/jb.180.13.3441-3447.1998}, number={13}, journal={Journal of Bacteriology}, publisher={American Society for Microbiology}, author={Kraft, Angelika R. and Templin, Markus F. and Höltje, Joachim-Volker}, year={1998}, month=jul, pages={3441–3447} }

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