Abstract
ABSTRACT Expression of the genes for the membrane-bound F o sector of the Escherichia coli F 1 F o proton-translocating ATPase can respond to changes in metabolic conditions, and these changes are reflected in alterations in the subunit stoichiometry of the oligomeric F o proton channel. Transcriptional and translational lacZ fusions to the promoter and to two F o genes show that, during growth on the nonfermentable carbon source succinate, transcription of the operon and translation of uncB , encoding the a subunit of F o , are higher than during growth on glucose. In contrast, translation of the uncE gene, encoding the c subunit of F o , is higher during growth on glucose than during growth on succinate. Translation rates of both uncB and uncE change as culture density increases, but transcription rates do not. Quantitation of the c stoichiometry shows that more c subunits are assembled into the F 1 F o ATPase in cells grown on glucose than in cells grown on succinate. E. coli therefore appears to have a mechanism for regulating the composition and, presumably, the function of the ATPase in response to metabolic circumstances.
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Dates
| Type | When |
|---|---|
| Created | 5 years, 7 months ago (Dec. 31, 2019, 11:24 a.m.) |
| Deposited | 4 years, 1 month ago (July 29, 2021, 2:22 p.m.) |
| Indexed | 3 weeks, 3 days ago (Aug. 5, 2025, 9:03 a.m.) |
| Issued | 27 years, 2 months ago (June 15, 1998) |
| Published | 27 years, 2 months ago (June 15, 1998) |
| Published Print | 27 years, 2 months ago (June 15, 1998) |
@article{Schemidt_1998, title={Effects of Carbon Source on Expression of F o Genes and on the Stoichiometry of the c Subunit in the F 1 F o ATPase of Escherichia coli}, volume={180}, ISSN={1098-5530}, url={http://dx.doi.org/10.1128/jb.180.12.3205-3208.1998}, DOI={10.1128/jb.180.12.3205-3208.1998}, number={12}, journal={Journal of Bacteriology}, publisher={American Society for Microbiology}, author={Schemidt, Randy A. and Qu, Jun and Williams, James R. and Brusilow, William S. A.}, year={1998}, month=jun, pages={3205–3208} }