DOI: 10.1128/jb.180.10.2736-2743.1998. Joint Transcriptional Control of<i>xpsR</i>, the Unusual Signal Integrator of the<i>Ralstonia solanacearum</i>Virulence Gene Regulatory Network, by a Response Regulator and a LysR-Type Transcriptional Activator

Joint Transcriptional Control ofxpsR, the Unusual Signal Integrator of theRalstonia solanacearumVirulence Gene Regulatory Network, by a Response Regulator and a LysR-Type Transcriptional Activator

10.1128/jb.180.10.2736-2743.1998

Crossref journal-article

American Society for Microbiology

Journal of Bacteriology (235)

Abstract

ABSTRACTRalstonia(Pseudomonas)solanacearumis a soil-borne phytopathogen that causes a wilting disease of many important crops. It makes large amounts of the exopolysaccharide EPS I, which it requires for efficient colonization, wilting, and killing of plants. Transcription of theepsoperon, encoding biosynthetic enzymes for EPS I, is controlled by a unique and complex sensory network that responds to multiple environmental signals. This network is comprised of the novel transcriptional activator XpsR, three distinct two-component regulatory systems (VsrAD, VsrBC, and PhcSR), and the LysR-type regulator PhcA, which is under the control of PhcSR. Here we show that thexpsRpromoter (PxpsR) is simultaneously controlled by PhcA and VsrD, permitting XpsR to act like a signal integrator, simultaneously coordinating signal input into theepspromoter from both VsrAD and PhcSR. Additionally, we used in vivo expression analysis and in vitro DNA binding assays with substitution and deletion mutants of PxpsRto show the following. (i) PhcA primarily interacts with a typical 14-bp LysR-type consensus sequence around position −77, causing a sixfold activation of PxpsR; a weaker, less-defined binding site between −183 and −239 likely enhances PhcA binding and activation via the −77 site another twofold. (ii) Full 70-fold activation of PxpsRrequires the additional interaction of the VsrD response regulator (or its surrogate) with a 14-bp dyadic sequence centered around −315 where it enhances activation (and possibly binding) by PhcA; however, VsrD alone cannot activate PxpsR. (iii) Increasing the distance between the putative VsrD binding site from that of PhcA by up to 232 bp did not dramatically affect PxpsRactivation or regulation.

Bibliography

Huang, J., Yindeeyoungyeon, W., Garg, R. P., Denny, T. P., & Schell, M. A. (1998). Joint Transcriptional Control ofxpsR, the Unusual Signal Integrator of theRalstonia solanacearumVirulence Gene Regulatory Network, by a Response Regulator and a LysR-Type Transcriptional Activator. Journal of Bacteriology, 180(10), 2736â2743.

Authors 5

Jianzhong Huang (first)
Wandee Yindeeyoungyeon (additional)
Ram P. Garg (additional)
Timothy P. Denny (additional)
Mark A. Schell (additional)

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Dates

Type	When
Created	5 years, 8 months ago (Dec. 31, 2019, 11:23 a.m.)
Deposited	1 year, 1 month ago (July 29, 2024, 8:09 a.m.)
Indexed	1 month, 3 weeks ago (July 14, 2025, 11:23 p.m.)
Issued	27 years, 3 months ago (May 15, 1998)
Published	27 years, 3 months ago (May 15, 1998)
Published Print	27 years, 3 months ago (May 15, 1998)

Funders 0

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BibTeX

@article{Huang_1998, title={Joint Transcriptional Control ofxpsR, the Unusual Signal Integrator of theRalstonia solanacearumVirulence Gene Regulatory Network, by a Response Regulator and a LysR-Type Transcriptional Activator}, volume={180}, ISSN={1098-5530}, url={http://dx.doi.org/10.1128/jb.180.10.2736-2743.1998}, DOI={10.1128/jb.180.10.2736-2743.1998}, number={10}, journal={Journal of Bacteriology}, publisher={American Society for Microbiology}, author={Huang, Jianzhong and Yindeeyoungyeon, Wandee and Garg, Ram P. and Denny, Timothy P. and Schell, Mark A.}, year={1998}, month=may, pages={2736–2743} }

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