Regulation of sugar uptake via the phosphoenolpyruvate-dependent phosphotransferase systems in Bacillus subtilis and Lactococcus lactis is mediated by ATP-dependent phosphorylation of seryl residue 46 in HPr
10.1128/jb.178.12.3557-3563.1996
Crossref journal-article
American Society for Microbiology
Journal of Bacteriology (235)
Abstract

By using both metabolizable and nonmetabolizable sugar substrates of the phosphoenolpyruvate-dependent phosphotransferase system (PTS), we show that PTS sugar uptake into intact cells and membrane vesicles of Lactococcus lactis and Bacillus subtilis is strongly inhibited by high concentrations of any of several metabolizable PTS sugars. Inhibition requires phosphorylation of seryl residue 46 in the phosphocarrier protein of the PTS, HPr, by the metabolite-activated, ATP-dependent protein kinase. Inhibition does not occur when wild-type HPr is replaced by the S46A mutant form of this protein either in vesicles of L. lactis or B. subtilis or in intact cells of B. subtilis. Nonmetabolizable PTS sugar analogs such as 2-deoxyglucose inhibit PTS sugar uptake by a distinct mechanism that is independent of HPr(ser-P) and probably involves cellular phosphoenolpyruvate depletion.

Bibliography

Ye, J. J., & Saier, M. H. (1996). Regulation of sugar uptake via the phosphoenolpyruvate-dependent phosphotransferase systems in Bacillus subtilis and Lactococcus lactis is mediated by ATP-dependent phosphorylation of seryl residue 46 in HPr. Journal of Bacteriology, 178(12), 3557–3563.

Authors 2
  1. J J Ye (first)
  2. M H Saier (additional)
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Dates
Type When
Created 8 years, 9 months ago (Nov. 14, 2016, 12:16 p.m.)
Deposited 4 years, 1 month ago (July 29, 2021, 1:57 p.m.)
Indexed 3 months, 2 weeks ago (May 18, 2025, 10:46 a.m.)
Issued 29 years, 3 months ago (June 1, 1996)
Published 29 years, 3 months ago (June 1, 1996)
Published Print 29 years, 3 months ago (June 1, 1996)
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@article{Ye_1996, title={Regulation of sugar uptake via the phosphoenolpyruvate-dependent phosphotransferase systems in Bacillus subtilis and Lactococcus lactis is mediated by ATP-dependent phosphorylation of seryl residue 46 in HPr}, volume={178}, ISSN={1098-5530}, url={http://dx.doi.org/10.1128/jb.178.12.3557-3563.1996}, DOI={10.1128/jb.178.12.3557-3563.1996}, number={12}, journal={Journal of Bacteriology}, publisher={American Society for Microbiology}, author={Ye, J J and Saier, M H}, year={1996}, month=jun, pages={3557–3563} }