DOI: 10.1128/jb.178.1.309-313.1996. Differentially transcribed regions of Haloferax volcanii genome depending on the medium salinity

Differentially transcribed regions of Haloferax volcanii genome depending on the medium salinity

10.1128/jb.178.1.309-313.1996

Crossref journal-article

American Society for Microbiology

Journal of Bacteriology (235)

Abstract

To identify genomic regions involved in osmoregulation in the extremely halophilic archaeon Haloferax volcanii, we used a technique which involves hybridization of cDNAs obtained at different salinities against a cosmid library of the organism. Both low and high salt concentrations trigger differential expression; however, adaptation to low salinities seems to elicit a wider response. The presence of a large domain within the largest of the megaplasmids with a strong response to low salt concentrations is noteworthy.

Bibliography

Ferrer, C., Mojica, F. J., Juez, G., & RodrÃguez-Valera, F. (1996). Differentially transcribed regions of Haloferax volcanii genome depending on the medium salinity. Journal of Bacteriology, 178(1), 309â313.

Authors 4

C Ferrer (first)
F J Mojica (additional)
G Juez (additional)
F Rodríguez-Valera (additional)

References 22 Referenced 21

Bayley , S. T. , and R. A. Morton . 1978 . Recent developments in the molecular biology of extremely halophilic bacteria . Crit. Rev. Microbiol. 11 : 151 - 205 . (10.3109/10408417809090622) / Crit. Rev. Microbiol. / Recent developments in the molecular biology of extremely halophilic bacteria by Bayley S. T. (1978)
Charlebois , R. L. , L. C. Schalkwyk , J. D. Hoffman , and W. F. Doolittle . 1991 . Detailed physical map and set of overlapping clones covering the genome of the archaebacterium Haloferax volcanii DS2 . J. Mol. Biol. 222 : 509 - 524 . (10.1016/0022-2836(91)90493-P) / J. Mol. Biol. / Detailed physical map and set of overlapping clones covering the genome of the archaebacterium Haloferax volcanii DS2 by Charlebois R. L. (1991)
Christian , J. H. B. , and J. A. Waltho . 1962 . Solute concentrations with cells of halophilic and non-halophilic bacteria . Biochim. Biophys. Acta 65 : 506 - 508 . (10.1016/0006-3002(62)90453-5) / Biochim. Biophys. Acta / Solute concentrations with cells of halophilic and non-halophilic bacteria by Christian J. H. B. (1962)
Chuang , S. - E. , D. L. Daniels , and F. R. Blattner . 1993 . Global regulation of gene expression in Escherichia coli . J. Bacteriol. 175 : 2026 - 2036 . (10.1128/jb.175.7.2026-2036.1993) / J. Bacteriol. / Global regulation of gene expression in Escherichia coli by Chuang S. (1993)
Cohen , A. , W. L. Lam , R. L. Charlebois , W. F. Doolittle , and L. C. Schalkwyk . 1992 . Localizing genes on the map of the genome of Haloferax volcanii, one of the archaea . Proc. Natl. Acad. Sci. USA 89 : 1602 - 1606 . (10.1073/pnas.89.5.1602) / Proc. Natl. Acad. Sci. USA / Localizing genes on the map of the genome of Haloferax volcanii, one of the archaea by Cohen A. (1992)
Csonka , L. N. , and A. D. Hanson . 1991 . Prokaryotic osmoregulation: genetics and physiology. Annu . Rev. Microbiol. 45 : 569 - 606 . (10.1146/annurev.mi.45.100191.003033) / Rev. Microbiol. / Prokaryotic osmoregulation: genetics and physiology. Annu by Csonka L. N. (1991)
Ferrer C. G. Juez and F. Rodríguez-Valera. Unpublished data.
Higgins , C. F. , C. J. Dorman , D. A. Stirling , L. Wadell , I. R. Booth , G. May , and E. Bremer . 1988 . A physiological role for DNA supercoiling in the osmotic regulation of gene expression in S. typhimurium and E. coli . Cell 52 : 569 - 584 . (10.1016/0092-8674(88)90470-9) / Cell / A physiological role for DNA supercoiling in the osmotic regulation of gene expression in S. typhimurium and E. coli by Higgins C. F. (1988)
Juez G. 1982. Aislamiento estudio taxonómico ultraestructural y molecular de nuevos grupos de halófilos extremos. Ph.D. thesis. University of Alicante Alicante Spain.
Juez G. 1988. Taxonomy of extremely halophilic archaebacteria p. 3-24. In F. Rodriguez-Valera (ed.) Halophilic bacteria vol. II. CRC Press Inc. Boca Raton Fla.
Lopez-García , P. , J. P. Abad , C. Smith , and R. Amils . 1992 . Genomic organization of the halophilic archaeon Haloferax mediterranei: physical map of the chromosome . Nucleic Acids Res. 20 : 2459 - 2464 . (10.1093/nar/20.10.2459) / Nucleic Acids Res. / Genomic organization of the halophilic archaeon Haloferax mediterranei: physical map of the chromosome by Lopez-García P. (1992)
Mizuno , T. , and S. Mizushima . 1990 . Signal transduction and gene regulation through the phosphorylation of two regulatory components: the molecular basis for the osmotic regulation of the genes . Mol. Microbiol. 4 : 1077 - 1082 . (10.1111/j.1365-2958.1990.tb00681.x) / Mol. Microbiol. / Signal transduction and gene regulation through the phosphorylation of two regulatory components: the molecular basis for the osmotic regulation of the genes by Mizuno T. (1990)
Mojica , F. J. M. , G. Juez , and F. Rodríguez-Valera . 1993 . Transcription at different salinities of Haloferax mediterranei sequences adjacent to partially modified PstI sites . Mol. Microbiol. 9 : 613 - 621 . (10.1111/j.1365-2958.1993.tb01721.x) / Mol. Microbiol. / Transcription at different salinities of Haloferax mediterranei sequences adjacent to partially modified PstI sites by Mojica F. J. M. (1993)
Mojica F. J. M. G. Juez and F. Rodriguez-Valera. Unpublished data.
Mullakhanbhai , M. F. , and H. Larsen . 1975 . Halobacterium volcanii spec. nov. a Dead Sea halobacterium with a moderate salt requirement . Arch. Microbiol. 104 : 207 - 214 . (10.1007/BF00447326) / Arch. Microbiol. / Halobacterium volcanii spec. nov. a Dead Sea halobacterium with a moderate salt requirement by Mullakhanbhai M. F. (1975)
Pfeifer F. 1988. Genetics of halobacteria p. 105-133. In F. Rodriguez-Valera (ed.) Halophilic bacteria vol. II. CRC Press Inc. Boca Raton Fla.
Rodríguez-Valera , F. , G. Juez , and D. J. Kushner . 1983 . Halobacterium mediterranei spec. nov., a new carbohydrate-utilizing extreme halophile . Syst. Appl. Microbiol. 4 : 369 - 381 . (10.1016/S0723-2020(83)80021-6) / Syst. Appl. Microbiol. / Halobacterium mediterranei spec. nov., a new carbohydrate-utilizing extreme halophile by Rodríguez-Valera F. (1983)
Rodriguez-Valera , F. , F. Ruiz-Berraquero , and A. Ramos-Cormenzana . 1980 . Behaviour of mixed populations of halophilic bacteria in continuous cultures . Can. J. Microbiol. 26 : 1259 - 1263 . (10.1139/m80-210) / Can. J. Microbiol. / Behaviour of mixed populations of halophilic bacteria in continuous cultures by Rodriguez-Valera F. (1980)
Rodríguez-Valera , F. , A. Ventosa , G. Juez , and J. F. Imhoff . 1985 . Variation of environmental features and microbial populations with salt concentrations in a multi-pond saltern . Microb. Ecol. 11 : 107 - 115 . (10.1007/BF02010483) / Microb. Ecol. / Variation of environmental features and microbial populations with salt concentrations in a multi-pond saltern by Rodríguez-Valera F. (1985)
Sambrook J. E. F. Fritsch and T. Maniatis. 1989. Molecular cloning: a laboratory manual 2nd ed. p. 8.3-8.67. Cold Spring Harbor Laboratory Press Cold Spring Harbor N.Y.
Trieselmann , B. A. , and R. L. Charlebois . 1992 . Transcriptionally active regions in the genome of the archaebacterium Haloferax volcanii . J. Bacteriol. 174 : 30 - 34 . (10.1128/jb.174.1.30-34.1992) / J. Bacteriol. / Transcriptionally active regions in the genome of the archaebacterium Haloferax volcanii by Trieselmann B. A. (1992)
Woese , C. R. , O. Kandler , and M. L. Wheelis . 1990 . Towards a natural system of organisms: proposal for the domains archaea, bacteria, and eucarya . Proc. Natl. Acad. Sci. USA 87 : 4576 - 4579 . (10.1073/pnas.87.12.4576) / Proc. Natl. Acad. Sci. USA / Towards a natural system of organisms: proposal for the domains archaea, bacteria, and eucarya by Woese C. R. (1990)

Dates

Type	When
Created	8 years, 9 months ago (Nov. 14, 2016, 5:49 a.m.)
Deposited	4 years, 1 month ago (July 29, 2021, 1:56 p.m.)
Indexed	3 weeks, 2 days ago (Aug. 6, 2025, 8:20 a.m.)
Issued	29 years, 7 months ago (Jan. 1, 1996)
Published	29 years, 7 months ago (Jan. 1, 1996)
Published Print	29 years, 7 months ago (Jan. 1, 1996)

Funders 0

None

BibTeX

@article{Ferrer_1996, title={Differentially transcribed regions of Haloferax volcanii genome depending on the medium salinity}, volume={178}, ISSN={1098-5530}, url={http://dx.doi.org/10.1128/jb.178.1.309-313.1996}, DOI={10.1128/jb.178.1.309-313.1996}, number={1}, journal={Journal of Bacteriology}, publisher={American Society for Microbiology}, author={Ferrer, C and Mojica, F J and Juez, G and Rodríguez-Valera, F}, year={1996}, month=jan, pages={309–313} }

JSON

{
  "indexed": {
    "date-parts": [
      [
        2025,
        8,
        6
      ]
    ],
    "date-time": "2025-08-06T12:20:13Z",
    "timestamp": 1754482813256
  },
  "reference-count": 22,
  "publisher": "American Society for Microbiology",
  "issue": "1",
  "license": [
    {
      "start": {
        "date-parts": [
          [
            1996,
            1,
            1
          ]
        ],
        "date-time": "1996-01-01T00:00:00Z",
        "timestamp": 820454400000
      },
      "content-version": "tdm",
      "delay-in-days": 0,
      "URL": "https://journals.asm.org/non-commercial-tdm-license"
    }
  ],
  "content-domain": {
    "domain": [
      "journals.asm.org"
    ],
    "crossmark-restriction": true
  },
  "published-print": {
    "date-parts": [
      [
        1996,
        1
      ]
    ]
  },
  "abstract": "<jats:p>To identify genomic regions involved in osmoregulation in the extremely halophilic archaeon Haloferax volcanii, we used a technique which involves hybridization of cDNAs obtained at different salinities against a cosmid library of the organism. Both low and high salt concentrations trigger differential expression; however, adaptation to low salinities seems to elicit a wider response. The presence of a large domain within the largest of the megaplasmids with a strong response to low salt concentrations is noteworthy.</jats:p>",
  "DOI": "10.1128/jb.178.1.309-313.1996",
  "type": "journal-article",
  "created": {
    "date-parts": [
      [
        2016,
        11,
        14
      ]
    ],
    "date-time": "2016-11-14T10:49:13Z",
    "timestamp": 1479120553000
  },
  "page": "309-313",
  "update-policy": "http://dx.doi.org/10.1128/asmj-crossmark-policy-page",
  "source": "Crossref",
  "is-referenced-by-count": 21,
  "title": "Differentially transcribed regions of Haloferax volcanii genome depending on the medium salinity",
  "prefix": "10.1128",
  "volume": "178",
  "author": [
    {
      "given": "C",
      "family": "Ferrer",
      "sequence": "first",
      "affiliation": [
        {
          "name": "Departamento de Gen\u00e9tica y Microbiolog\u00eda, Universidad de Alicante, Spain."
        }
      ]
    },
    {
      "given": "F J",
      "family": "Mojica",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "Departamento de Gen\u00e9tica y Microbiolog\u00eda, Universidad de Alicante, Spain."
        }
      ]
    },
    {
      "given": "G",
      "family": "Juez",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "Departamento de Gen\u00e9tica y Microbiolog\u00eda, Universidad de Alicante, Spain."
        }
      ]
    },
    {
      "given": "F",
      "family": "Rodr\u00edguez-Valera",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "Departamento de Gen\u00e9tica y Microbiolog\u00eda, Universidad de Alicante, Spain."
        }
      ]
    }
  ],
  "member": "235",
  "reference": [
    {
      "key": "p_1",
      "doi-asserted-by": "crossref",
      "first-page": "151",
      "DOI": "10.3109/10408417809090622",
      "article-title": "Recent developments in the molecular biology of extremely halophilic bacteria",
      "volume": "11",
      "author": "Bayley S. T.",
      "year": "1978",
      "unstructured": "Bayley , S. T. , and R. A. Morton . 1978 . Recent developments in the molecular biology of extremely halophilic bacteria . Crit. Rev. Microbiol. 11 : 151 - 205 .",
      "journal-title": "Crit. Rev. Microbiol."
    },
    {
      "key": "p_2",
      "doi-asserted-by": "crossref",
      "first-page": "509",
      "DOI": "10.1016/0022-2836(91)90493-P",
      "article-title": "Detailed physical map and set of overlapping clones covering the genome of the archaebacterium Haloferax volcanii DS2",
      "volume": "222",
      "author": "Charlebois R. L.",
      "year": "1991",
      "unstructured": "Charlebois , R. L. , L. C. Schalkwyk , J. D. Hoffman , and W. F. Doolittle . 1991 . Detailed physical map and set of overlapping clones covering the genome of the archaebacterium Haloferax volcanii DS2 . J. Mol. Biol. 222 : 509 - 524 .",
      "journal-title": "J. Mol. Biol."
    },
    {
      "key": "p_3",
      "doi-asserted-by": "crossref",
      "first-page": "506",
      "DOI": "10.1016/0006-3002(62)90453-5",
      "article-title": "Solute concentrations with cells of halophilic and non-halophilic bacteria",
      "volume": "65",
      "author": "Christian J. H. B.",
      "year": "1962",
      "unstructured": "Christian , J. H. B. , and J. A. Waltho . 1962 . Solute concentrations with cells of halophilic and non-halophilic bacteria . Biochim. Biophys. Acta 65 : 506 - 508 .",
      "journal-title": "Biochim. Biophys. Acta"
    },
    {
      "key": "p_4",
      "doi-asserted-by": "crossref",
      "first-page": "2026",
      "DOI": "10.1128/jb.175.7.2026-2036.1993",
      "article-title": "Global regulation of gene expression in Escherichia coli",
      "volume": "175",
      "author": "Chuang S.",
      "year": "1993",
      "unstructured": "Chuang , S. - E. , D. L. Daniels , and F. R. Blattner . 1993 . Global regulation of gene expression in Escherichia coli . J. Bacteriol. 175 : 2026 - 2036 .",
      "journal-title": "J. Bacteriol."
    },
    {
      "key": "p_5",
      "doi-asserted-by": "crossref",
      "first-page": "1602",
      "DOI": "10.1073/pnas.89.5.1602",
      "article-title": "Localizing genes on the map of the genome of Haloferax volcanii, one of the archaea",
      "volume": "89",
      "author": "Cohen A.",
      "year": "1992",
      "unstructured": "Cohen , A. , W. L. Lam , R. L. Charlebois , W. F. Doolittle , and L. C. Schalkwyk . 1992 . Localizing genes on the map of the genome of Haloferax volcanii, one of the archaea . Proc. Natl. Acad. Sci. USA 89 : 1602 - 1606 .",
      "journal-title": "Proc. Natl. Acad. Sci. USA"
    },
    {
      "key": "p_6",
      "doi-asserted-by": "crossref",
      "first-page": "569",
      "DOI": "10.1146/annurev.mi.45.100191.003033",
      "article-title": "Prokaryotic osmoregulation: genetics and physiology. Annu",
      "volume": "45",
      "author": "Csonka L. N.",
      "year": "1991",
      "unstructured": "Csonka , L. N. , and A. D. Hanson . 1991 . Prokaryotic osmoregulation: genetics and physiology. Annu . Rev. Microbiol. 45 : 569 - 606 .",
      "journal-title": "Rev. Microbiol."
    },
    {
      "key": "p_7",
      "unstructured": "Ferrer C. G. Juez and F. Rodr\u00edguez-Valera. Unpublished data."
    },
    {
      "key": "p_8",
      "doi-asserted-by": "crossref",
      "first-page": "569",
      "DOI": "10.1016/0092-8674(88)90470-9",
      "article-title": "A physiological role for DNA supercoiling in the osmotic regulation of gene expression in S. typhimurium and E. coli",
      "volume": "52",
      "author": "Higgins C. F.",
      "year": "1988",
      "unstructured": "Higgins , C. F. , C. J. Dorman , D. A. Stirling , L. Wadell , I. R. Booth , G. May , and E. Bremer . 1988 . A physiological role for DNA supercoiling in the osmotic regulation of gene expression in S. typhimurium and E. coli . Cell 52 : 569 - 584 .",
      "journal-title": "Cell"
    },
    {
      "key": "p_9",
      "unstructured": "Juez G. 1982. Aislamiento estudio taxon\u00f3mico ultraestructural y molecular de nuevos grupos de hal\u00f3filos extremos. Ph.D. thesis. University of Alicante Alicante Spain."
    },
    {
      "key": "p_10",
      "unstructured": "Juez G. 1988. Taxonomy of extremely halophilic archaebacteria p. 3-24. In F. Rodriguez-Valera (ed.) Halophilic bacteria vol. II. CRC Press Inc. Boca Raton Fla."
    },
    {
      "key": "p_11",
      "doi-asserted-by": "crossref",
      "first-page": "2459",
      "DOI": "10.1093/nar/20.10.2459",
      "article-title": "Genomic organization of the halophilic archaeon Haloferax mediterranei: physical map of the chromosome",
      "volume": "20",
      "author": "Lopez-Garc\u00eda P.",
      "year": "1992",
      "unstructured": "Lopez-Garc\u00eda , P. , J. P. Abad , C. Smith , and R. Amils . 1992 . Genomic organization of the halophilic archaeon Haloferax mediterranei: physical map of the chromosome . Nucleic Acids Res. 20 : 2459 - 2464 .",
      "journal-title": "Nucleic Acids Res."
    },
    {
      "key": "p_12",
      "doi-asserted-by": "crossref",
      "first-page": "1077",
      "DOI": "10.1111/j.1365-2958.1990.tb00681.x",
      "article-title": "Signal transduction and gene regulation through the phosphorylation of two regulatory components: the molecular basis for the osmotic regulation of the genes",
      "volume": "4",
      "author": "Mizuno T.",
      "year": "1990",
      "unstructured": "Mizuno , T. , and S. Mizushima . 1990 . Signal transduction and gene regulation through the phosphorylation of two regulatory components: the molecular basis for the osmotic regulation of the genes . Mol. Microbiol. 4 : 1077 - 1082 .",
      "journal-title": "Mol. Microbiol."
    },
    {
      "key": "p_13",
      "doi-asserted-by": "crossref",
      "first-page": "613",
      "DOI": "10.1111/j.1365-2958.1993.tb01721.x",
      "article-title": "Transcription at different salinities of Haloferax mediterranei sequences adjacent to partially modified PstI sites",
      "volume": "9",
      "author": "Mojica F. J. M.",
      "year": "1993",
      "unstructured": "Mojica , F. J. M. , G. Juez , and F. Rodr\u00edguez-Valera . 1993 . Transcription at different salinities of Haloferax mediterranei sequences adjacent to partially modified PstI sites . Mol. Microbiol. 9 : 613 - 621 .",
      "journal-title": "Mol. Microbiol."
    },
    {
      "key": "p_14",
      "unstructured": "Mojica F. J. M. G. Juez and F. Rodriguez-Valera. Unpublished data."
    },
    {
      "key": "p_15",
      "doi-asserted-by": "crossref",
      "first-page": "207",
      "DOI": "10.1007/BF00447326",
      "article-title": "Halobacterium volcanii spec. nov. a Dead Sea halobacterium with a moderate salt requirement",
      "volume": "104",
      "author": "Mullakhanbhai M. F.",
      "year": "1975",
      "unstructured": "Mullakhanbhai , M. F. , and H. Larsen . 1975 . Halobacterium volcanii spec. nov. a Dead Sea halobacterium with a moderate salt requirement . Arch. Microbiol. 104 : 207 - 214 .",
      "journal-title": "Arch. Microbiol."
    },
    {
      "key": "p_16",
      "unstructured": "Pfeifer F. 1988. Genetics of halobacteria p. 105-133. In F. Rodriguez-Valera (ed.) Halophilic bacteria vol. II. CRC Press Inc. Boca Raton Fla."
    },
    {
      "key": "p_17",
      "doi-asserted-by": "crossref",
      "first-page": "369",
      "DOI": "10.1016/S0723-2020(83)80021-6",
      "article-title": "Halobacterium mediterranei spec. nov., a new carbohydrate-utilizing extreme halophile",
      "volume": "4",
      "author": "Rodr\u00edguez-Valera F.",
      "year": "1983",
      "unstructured": "Rodr\u00edguez-Valera , F. , G. Juez , and D. J. Kushner . 1983 . Halobacterium mediterranei spec. nov., a new carbohydrate-utilizing extreme halophile . Syst. Appl. Microbiol. 4 : 369 - 381 .",
      "journal-title": "Syst. Appl. Microbiol."
    },
    {
      "key": "p_18",
      "doi-asserted-by": "crossref",
      "first-page": "1259",
      "DOI": "10.1139/m80-210",
      "article-title": "Behaviour of mixed populations of halophilic bacteria in continuous cultures",
      "volume": "26",
      "author": "Rodriguez-Valera F.",
      "year": "1980",
      "unstructured": "Rodriguez-Valera , F. , F. Ruiz-Berraquero , and A. Ramos-Cormenzana . 1980 . Behaviour of mixed populations of halophilic bacteria in continuous cultures . Can. J. Microbiol. 26 : 1259 - 1263 .",
      "journal-title": "Can. J. Microbiol."
    },
    {
      "key": "p_19",
      "doi-asserted-by": "crossref",
      "first-page": "107",
      "DOI": "10.1007/BF02010483",
      "article-title": "Variation of environmental features and microbial populations with salt concentrations in a multi-pond saltern",
      "volume": "11",
      "author": "Rodr\u00edguez-Valera F.",
      "year": "1985",
      "unstructured": "Rodr\u00edguez-Valera , F. , A. Ventosa , G. Juez , and J. F. Imhoff . 1985 . Variation of environmental features and microbial populations with salt concentrations in a multi-pond saltern . Microb. Ecol. 11 : 107 - 115 .",
      "journal-title": "Microb. Ecol."
    },
    {
      "key": "p_20",
      "unstructured": "Sambrook J. E. F. Fritsch and T. Maniatis. 1989. Molecular cloning: a laboratory manual 2nd ed. p. 8.3-8.67. Cold Spring Harbor Laboratory Press Cold Spring Harbor N.Y."
    },
    {
      "key": "p_21",
      "doi-asserted-by": "crossref",
      "first-page": "30",
      "DOI": "10.1128/jb.174.1.30-34.1992",
      "article-title": "Transcriptionally active regions in the genome of the archaebacterium Haloferax volcanii",
      "volume": "174",
      "author": "Trieselmann B. A.",
      "year": "1992",
      "unstructured": "Trieselmann , B. A. , and R. L. Charlebois . 1992 . Transcriptionally active regions in the genome of the archaebacterium Haloferax volcanii . J. Bacteriol. 174 : 30 - 34 .",
      "journal-title": "J. Bacteriol."
    },
    {
      "key": "p_22",
      "doi-asserted-by": "crossref",
      "first-page": "4576",
      "DOI": "10.1073/pnas.87.12.4576",
      "article-title": "Towards a natural system of organisms: proposal for the domains archaea, bacteria, and eucarya",
      "volume": "87",
      "author": "Woese C. R.",
      "year": "1990",
      "unstructured": "Woese , C. R. , O. Kandler , and M. L. Wheelis . 1990 . Towards a natural system of organisms: proposal for the domains archaea, bacteria, and eucarya . Proc. Natl. Acad. Sci. USA 87 : 4576 - 4579 .",
      "journal-title": "Proc. Natl. Acad. Sci. USA"
    }
  ],
  "container-title": "Journal of Bacteriology",
  "original-title": [],
  "language": "en",
  "link": [
    {
      "URL": "https://journals.asm.org/doi/pdf/10.1128/jb.178.1.309-313.1996",
      "content-type": "application/pdf",
      "content-version": "vor",
      "intended-application": "text-mining"
    },
    {
      "URL": "https://journals.asm.org/doi/pdf/10.1128/jb.178.1.309-313.1996",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "similarity-checking"
    }
  ],
  "deposited": {
    "date-parts": [
      [
        2021,
        7,
        29
      ]
    ],
    "date-time": "2021-07-29T17:56:05Z",
    "timestamp": 1627581365000
  },
  "score": 1,
  "resource": {
    "primary": {
      "URL": "https://journals.asm.org/doi/10.1128/jb.178.1.309-313.1996"
    }
  },
  "subtitle": [],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        1996,
        1
      ]
    ]
  },
  "references-count": 22,
  "journal-issue": {
    "issue": "1",
    "published-print": {
      "date-parts": [
        [
          1996,
          1
        ]
      ]
    }
  },
  "alternative-id": [
    "10.1128/jb.178.1.309-313.1996"
  ],
  "URL": "http://dx.doi.org/10.1128/JB.178.1.309-313.1996",
  "relation": {},
  "ISSN": [
    "0021-9193",
    "1098-5530"
  ],
  "subject": [],
  "container-title-short": "J Bacteriol",
  "published": {
    "date-parts": [
      [
        1996,
        1
      ]
    ]
  },
  "assertion": [
    {
      "value": "2021-01-31",
      "order": 2,
      "name": "published",
      "label": "Published",
      "group": {
        "name": "publication_history",
        "label": "Publication History"
      }
    }
  ]
}