The sea pansy Renilla reniformis luciferase serves as a sensitive bioluminescent reporter for differential gene expression in Candida albicans
10.1128/jb.178.1.121-129.1996
Crossref journal-article
American Society for Microbiology
Journal of Bacteriology (235)
Abstract

The infectious yeast Candida albicans progresses through two developmental programs which involve differential gene expression, the bud-hypha transition and high-frequency phenotypic switching. To understand how differentially expressed genes are regulated in this organism, the promoters of phase-specific genes must be functionally characterized, and a bioluminescent reporter system would facilitate such characterization. However, C. albicans has adopted a nontraditional codon strategy that involves a tRNA with a CAG anticodon to decode the codon CUG as serine rather than leucine. Since the luciferase gene of the sea pansy Renilla reinformis contains no CUGs, we have used it to develop a highly sensitive bioluminescent reporter system for C. albicans. When fused to the galactose-inducible promoter of GAL1, luciferase activity is inducible; when fused to the constitutive EF1 alpha 2 promoter, luciferase activity is constitutive; and when fused to the promoter of the white-phase-specific gene WH11 or the opaque-phase-specific gene OP4, luciferase activity is phase specific. The Renilla luciferase system can, therefore, be used as a bioluminescent reporter to analyze the strength and developmental regulation of C. albicans promoters.

Bibliography

Srikantha, T., Klapach, A., Lorenz, W. W., Tsai, L. K., Laughlin, L. A., Gorman, J. A., & Soll, D. R. (1996). The sea pansy Renilla reniformis luciferase serves as a sensitive bioluminescent reporter for differential gene expression in Candida albicans. Journal of Bacteriology, 178(1), 121–129.

Authors 7
  1. T Srikantha (first)
  2. A Klapach (additional)
  3. W W Lorenz (additional)
  4. L K Tsai (additional)
  5. L A Laughlin (additional)
  6. J A Gorman (additional)
  7. D R Soll (additional)
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Dates
Type When
Created 8 years, 9 months ago (Nov. 14, 2016, 5:49 a.m.)
Deposited 4 years ago (July 29, 2021, 1:56 p.m.)
Indexed 1 month ago (July 19, 2025, 11:55 p.m.)
Issued 29 years, 7 months ago (Jan. 1, 1996)
Published 29 years, 7 months ago (Jan. 1, 1996)
Published Print 29 years, 7 months ago (Jan. 1, 1996)
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@article{Srikantha_1996, title={The sea pansy Renilla reniformis luciferase serves as a sensitive bioluminescent reporter for differential gene expression in Candida albicans}, volume={178}, ISSN={1098-5530}, url={http://dx.doi.org/10.1128/jb.178.1.121-129.1996}, DOI={10.1128/jb.178.1.121-129.1996}, number={1}, journal={Journal of Bacteriology}, publisher={American Society for Microbiology}, author={Srikantha, T and Klapach, A and Lorenz, W W and Tsai, L K and Laughlin, L A and Gorman, J A and Soll, D R}, year={1996}, month=jan, pages={121–129} }