VirG, a Yersinia enterocolitica lipoprotein involved in Ca2+ dependency, is related to exsB of Pseudomonas aeruginosa
10.1128/jb.177.15.4230-4237.1995
Crossref journal-article
American Society for Microbiology
Journal of Bacteriology (235)
Abstract

Pathogenic yersiniae require Ca2+ for growth at 37 degrees C. They harbor closely related plasmids of about 70 kb that are essential for virulence. At 37 degrees C and in the absence of Ca2+ ions, these plasmids cause a decrease in growth rate and the release of large amounts of proteins called Yops. Here we describe the virG gene of Yersinia enterocolitica; virG is located just upstream of the virF gene, which encodes the transcriptional activator of some plasmid virulence factors. Analysis of the VirG amino acid sequence suggested that virG encodes a lipoprotein, which was confirmed by [3H]palmitate labeling of VirG-PhoA fusion proteins. A nonpolar virG mutant was constructed and found to be Ca2+ independent for growth at 37 degrees C but to still secrete Yops. This phenotype was complemented by the introduction of a plasmid harboring an intact virG gene. VirG was found to be homologous to ExsB, a protein encoded by a Pseudomonas aeruginosa gene located in the locus controlling exoenzyme S synthesis. Interestingly, the exsA gene, located just downstream of exsB, is also homologous to virF.

Bibliography

Allaoui, A., Scheen, R., Lambert de Rouvroit, C., & Cornelis, G. R. (1995). VirG, a Yersinia enterocolitica lipoprotein involved in Ca2+ dependency, is related to exsB of Pseudomonas aeruginosa. Journal of Bacteriology, 177(15), 4230–4237.

Authors 4
  1. A Allaoui (first)
  2. R Scheen (additional)
  3. C Lambert de Rouvroit (additional)
  4. G R Cornelis (additional)
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Dates
Type When
Created 8 years, 9 months ago (Nov. 9, 2016, 9:04 a.m.)
Deposited 4 years ago (July 29, 2021, 1:50 p.m.)
Indexed 1 year, 6 months ago (Feb. 9, 2024, 4:23 p.m.)
Issued 30 years ago (Aug. 1, 1995)
Published 30 years ago (Aug. 1, 1995)
Published Print 30 years ago (Aug. 1, 1995)
Funders 0

None

@article{Allaoui_1995, title={VirG, a Yersinia enterocolitica lipoprotein involved in Ca2+ dependency, is related to exsB of Pseudomonas aeruginosa}, volume={177}, ISSN={1098-5530}, url={http://dx.doi.org/10.1128/jb.177.15.4230-4237.1995}, DOI={10.1128/jb.177.15.4230-4237.1995}, number={15}, journal={Journal of Bacteriology}, publisher={American Society for Microbiology}, author={Allaoui, A and Scheen, R and Lambert de Rouvroit, C and Cornelis, G R}, year={1995}, month=aug, pages={4230–4237} }