Synthesis of F pilin
10.1128/jb.175.5.1384-1391.1993
Crossref journal-article
American Society for Microbiology
Journal of Bacteriology (235)
Abstract

Transfer of the Escherichia coli fertility plasmid, F, is dependent on expression of F pili. Synthesis of F-pilin subunits is known to involve three F plasmid transfer (tra) region products: traA encodes the 13-kDa precursor protein, TraQ permits this to be processed to the 7-kDa pilin polypeptide, and TraX catalyzes acetylation of the pilin amino terminus. Using cloned tra sequences, we performed a series of pulse-chase experiments to investigate the effect of TraQ and TraX on the fate of the traA product. In TraQ- cells, the traA gene product was found to be very unstable. While traA polypeptides of various sizes were detected early in the chase period, almost all were degraded within 5 min. Rapid traA product degradation was also observed in TraX+ cells, although an increased percentage of these products persisted during the chase. In TraQ+ cells, most of the traA product was processed to the 7-kDa pilin polypeptide within the 1-min pulse period; this product [7(Q)] was not degraded but was increasingly converted to an 8-kDa form [8(Q)] as the chase continued, suggesting that host enzymes can modify the pilin polypeptide. Similar results were observed in TraQ+ TraX+ cells, but the primary 7-kDa product appeared to be N-acetylated pilin (Ac-7). An 8-kDa product (Ac-8) was also detected, but this band did not increase in intensity during the chase. We suggest a pathway in which TraQ prevents the traA product from folding to a readily degradable conformation and assists its entry into the membrane, Leader peptidase I cleaves the traA product signal sequence, and a subset of the pilin polypeptides becomes modified by host enzymes; TraX then acetylates the N terminal of both the modified and unmodified pilin polypeptides.

Bibliography

Maneewannakul, K., Maneewannakul, S., & Ippen-Ihler, K. (1993). Synthesis of F pilin. Journal of Bacteriology, 175(5), 1384–1391.

Authors 3
  1. K Maneewannakul (first)
  2. S Maneewannakul (additional)
  3. K Ippen-Ihler (additional)
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Dates
Type When
Created 8 years, 9 months ago (Nov. 7, 2016, 10:42 a.m.)
Deposited 4 years, 1 month ago (July 29, 2021, 1:17 p.m.)
Indexed 1 year, 1 month ago (July 4, 2024, 11:21 a.m.)
Issued 32 years, 6 months ago (March 1, 1993)
Published 32 years, 6 months ago (March 1, 1993)
Published Print 32 years, 6 months ago (March 1, 1993)
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@article{Maneewannakul_1993, title={Synthesis of F pilin}, volume={175}, ISSN={1098-5530}, url={http://dx.doi.org/10.1128/jb.175.5.1384-1391.1993}, DOI={10.1128/jb.175.5.1384-1391.1993}, number={5}, journal={Journal of Bacteriology}, publisher={American Society for Microbiology}, author={Maneewannakul, K and Maneewannakul, S and Ippen-Ihler, K}, year={1993}, month=mar, pages={1384–1391} }