Genetic analysis of the agrocinopine catabolic region of Agrobacterium tumefaciens Ti plasmid pTiC58, which encodes genes required for opine and agrocin 84 transport
10.1128/jb.175.17.5575-5584.1993
Crossref journal-article
American Society for Microbiology
Journal of Bacteriology (235)
Abstract

The acc region, subcloned from pTiC58 of classical nopaline and agrocinopine A and B Agrobacterium tumefaciens C58, allowed agrobacteria to grow using agrocinopine B as the sole source of carbon and energy. acc is approximately 6 kb in size. It consists of at least five genes, accA through accE, as defined by complementation analysis using subcloned fragments and transposon insertion mutations of acc carried on different plasmids within the same cell. All five regions are required for agrocin 84 sensitivity, and at least four are required for agrocinopine and agrocin 84 uptake. The complementation results are consistent with the hypothesis that each of the five regions is separately transcribed. Maxicell experiments showed that the first of these genes, accA, encodes a 60-kDa protein. Analysis of osmotic shock fractions showed this protein to be located in the periplasm. The DNA sequence of the accA region revealed an open reading frame encoding a predicted polypeptide of 59,147 Da. The amino acid sequence encoded by this open reading frame is similar to the periplasmic binding proteins OppA and DppA of Escherichia coli and Salmonella typhimurium and OppA of Bacillus subtilis.

Bibliography

Hayman, G. T., Beck von Bodman, S., Kim, H., Jiang, P., & Farrand, S. K. (1993). Genetic analysis of the agrocinopine catabolic region of Agrobacterium tumefaciens Ti plasmid pTiC58, which encodes genes required for opine and agrocin 84 transport. Journal of Bacteriology, 175(17), 5575–5584.

Authors 5
  1. G T Hayman (first)
  2. S Beck von Bodman (additional)
  3. H Kim (additional)
  4. P Jiang (additional)
  5. S K Farrand (additional)
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Dates
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Created 8 years, 9 months ago (Nov. 7, 2016, 3:22 p.m.)
Deposited 4 years, 1 month ago (July 29, 2021, 1:52 p.m.)
Indexed 1 year, 2 months ago (June 10, 2024, 9:18 a.m.)
Issued 32 years ago (Sept. 1, 1993)
Published 32 years ago (Sept. 1, 1993)
Published Print 32 years ago (Sept. 1, 1993)
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@article{Hayman_1993, title={Genetic analysis of the agrocinopine catabolic region of Agrobacterium tumefaciens Ti plasmid pTiC58, which encodes genes required for opine and agrocin 84 transport}, volume={175}, ISSN={1098-5530}, url={http://dx.doi.org/10.1128/jb.175.17.5575-5584.1993}, DOI={10.1128/jb.175.17.5575-5584.1993}, number={17}, journal={Journal of Bacteriology}, publisher={American Society for Microbiology}, author={Hayman, G T and Beck von Bodman, S and Kim, H and Jiang, P and Farrand, S K}, year={1993}, month=sep, pages={5575–5584} }