DOI: 10.1128/jb.173.3.1139-1144.1991. Controlled expression of the transcriptional activator gene virG in Agrobacterium tumefaciens by using the Escherichia coli lac promoter

Controlled expression of the transcriptional activator gene virG in Agrobacterium tumefaciens by using the Escherichia coli lac promoter

10.1128/jb.173.3.1139-1144.1991

Crossref journal-article

American Society for Microbiology

Journal of Bacteriology (235)

Abstract

The Agrobacterium VirG protein is normally expressed from two promoters in response to multiple stimuli, including plant-released phenolics (at promoter P1) and acidic growth media (at promoter P2). To simplify the analysis of vir gene induction, we sought to create Agrobacterium strains in which virG could be expressed in a controllable fashion. To study the possibility of using the lac promoter and repressor, we constructed a plasmid containing the lac promoter fused to the lacZ structural gene. A derivative of this plasmid containing the lacIq gene was also constructed. The plasmid not containing lacIq expressed high levels of beta-galactosidase. The plasmid containing lacIq expressed beta-galactosidase at very low levels in the absence of o-nitrophenyl-beta-D-galactoside (IPTG) and at moderate levels in the presence of IPTG. We also fused the lac promoter to a virG::lacZ translational fusion and found that IPTG elevated expression of this translational fusion to moderate levels, though not to levels as high as from the stronger of the two native virG promoters. Finally, the lac promoter was used to express the native virG gene in strains containing a virB::lacZ translational fusion. virB expression in this strain depended on addition of IPTG as well as the vir gene inducer acetosyringone. In a similar strain lacking lacIq, virB expression was greater than in a strain in which virG was expressed from its native promoters. Expression of virG from the lac promoter did not alter the acidic pH optimum for vir gene induction, indicating that the previously observed requirement for acidic media was not due solely to the need to induce P2.

Bibliography

Chen, C. Y., & Winans, S. C. (1991). Controlled expression of the transcriptional activator gene virG in Agrobacterium tumefaciens by using the Escherichia coli lac promoter. Journal of Bacteriology, 173(3), 1139â1144.

Authors 2

C Y Chen (first)
S C Winans (additional)

References 29 Referenced 104

Ankenbauer , R. G. , and E. W. Nester . 1990 . Sugar-mediated induction of Agrobacterium tumefaciens virulence genes: structural specificity and activities of monosaccharides . J. Bacteriol. 172 : 6442 - 6446 . (10.1128/jb.172.11.6442-6446.1990) / J. Bacteriol. / Sugar-mediated induction of Agrobacterium tumefaciens virulence genes: structural specificity and activities of monosaccharides by Ankenbauer R. G. (1990)
Bagdasarian , M. M. , E. Amann , R. Lurz , B. Ruckert , and M. Bagdasarian . 1983 . Activity of the hybrid trp-lac (tac) promoter of Escherichia coli in Pseudomonas putida. Construction of broad-host-range, controlled-expression vectors . Gene 26 : 273 - 282 . (10.1016/0378-1119(83)90197-X) / Gene / Activity of the hybrid trp-lac (tac) promoter of Escherichia coli in Pseudomonas putida. Construction of broad-host-range, controlled-expression vectors by Bagdasarian M. M. (1983)
Cangelosi , G. A. , R. G. Ankenbauer , and E. W. Nester . 1990 . Sugars induce the Agrobacterium virulence genes through a periplasmic binding protein and a transmembrane signal protein . Proc. Natl. Acad. Sci. USA 87 : 6708 - 6712 . (10.1073/pnas.87.17.6708) / Proc. Natl. Acad. Sci. USA / Sugars induce the Agrobacterium virulence genes through a periplasmic binding protein and a transmembrane signal protein by Cangelosi G. A. (1990)
Casadaban , M. J. , J. Chou , and S. N. Cohen . 1980 . In vitro gene fusions that join an enzymatically active P-galactosidase segment to amino terminal fragments of exogenous proteins: E. coli plasmid vectors for the detection and cloning of translational initiation signals . J. Bacteriol. 143 : 971 - 980 . (10.1128/jb.143.2.971-980.1980) / J. Bacteriol. / In vitro gene fusions that join an enzymatically active P-galactosidase segment to amino terminal fragments of exogenous proteins: E. coli plasmid vectors for the detection and cloning of translational initiation signals by Casadaban M. J. (1980)
Chilton , M. - D. , T. Currier , S. Farrand , A. J. Benddich , M. P. Gordon , and E. W. Nester . 1974 . Agrobacterium tumefaciens DNA and PS8 bacteriophage DNA not detected in crown gall tumors . Proc. Natl. Acad. Sci. USA 71 : 3672 - 3676 . (10.1073/pnas.71.9.3672) / Proc. Natl. Acad. Sci. USA / Agrobacterium tumefaciens DNA and PS8 bacteriophage DNA not detected in crown gall tumors by Chilton M. (1974)
Close , T. J. , P. M. Rogowsky , C. I. Kado , S. C. Winans , M. F. Yanofsky , and E. W. Nester . 1987 . Dual control of the Agrobacterium tumefaciens Ti plasmid virulence genes . J. Bacteriol. 169 : 5113 - 5118 . (10.1128/jb.169.11.5113-5118.1987) / J. Bacteriol. / Dual control of the Agrobacterium tumefaciens Ti plasmid virulence genes by Close T. J. (1987)
Close , T. J. , D. Zaitlin , and C. I. Kado . 1984 . Design and development of amplifiable broad-host-range cloning vectors: analysis of the vir region of Agrobacterium tumefaciens plasmid pTiC58 . Plasmid 12 : 111 - 118 . (10.1016/0147-619X(84)90057-X) / Plasmid / Design and development of amplifiable broad-host-range cloning vectors: analysis of the vir region of Agrobacterium tumefaciens plasmid pTiC58 by Close T. J. (1984)
Cowing , D. W. , J. C. A. Bardwell , E. A. Craig , C. Woolford , R. W. Hendrix , and C. A. Gross . 1985 . Consensus sequence for Escherichia coli heat shock promoters . Proc. Natl. Acad. Sci. USA 82 : 2697 - 2683 . (10.1073/pnas.82.9.2679) / Proc. Natl. Acad. Sci. USA / Consensus sequence for Escherichia coli heat shock promoters by Cowing D. W. (1985)
Jin , S. , T. Roitsch , P. J. Christie , and E. W. Nester . 1990 . The regulatory VirG protein specifically binds to a cis-acting regulatory sequence involved in transcriptional activation of Agrobacterium tumefaciens virulence genes . J. Bacteriol. 172 : 531 - 537 . (10.1128/jb.172.2.531-537.1990) / J. Bacteriol. / The regulatory VirG protein specifically binds to a cis-acting regulatory sequence involved in transcriptional activation of Agrobacterium tumefaciens virulence genes by Jin S. (1990)
Klee , H. J. , M. F. Yanofsky , and E. W. Nester . 1985 . Vectors for transformation of higher plants . Bio/Technology 3 : 637 - 642 . / Bio/Technology / Vectors for transformation of higher plants by Klee H. J. (1985)
Leroux , B. , M. F. Yanofsky , S. C. Winans , J. E. Ward , S. F. Ziegler , and E. W. Nester . 1987 . Characterization of the virA locus of Agrobacterium tumefaciens: a transcriptional regulator and host range determinant . EMBO J. 6 : 849 - 856 . (10.1002/j.1460-2075.1987.tb04830.x) / EMBO J. / Characterization of the virA locus of Agrobacterium tumefaciens: a transcriptional regulator and host range determinant by Leroux B. (1987)
.Mantis N. M. Sessler and S. Winans. Unpublished data.
Marsh , J. L. , M. Erfle , and E. J. Wykes . 1984 . The pIC plasmid and phage vectors with versatile cloning sites for recombinant selection by insertional inactivation . Gene 32 : 481 - 485 . (10.1016/0378-1119(84)90022-2) / Gene / The pIC plasmid and phage vectors with versatile cloning sites for recombinant selection by insertional inactivation by Marsh J. L. (1984)
Melchers , L. S. , T. J. G. Regensburg-Tuink , R. B. Bourret , N. J. A. Sedee , R. A. Schilperoort , and P. J. J. Hooykaas . 1989 . Membrane topology and functional analysis of the sensory protein VirA of Agrobacterium tumefaciens . EMBO J. 8 : 1919 - 1925 . (10.1002/j.1460-2075.1989.tb03595.x) / EMBO J. / Membrane topology and functional analysis of the sensory protein VirA of Agrobacterium tumefaciens by Melchers L. S. (1989)
Miller J. H. 1972. Experiments in molecular genetics. Cold Spring Harbor Laboratory Cold Spring Harbor N.Y.
Pazour , G. J. , and A. Das . 1990 . virG, an Agrobacterium tumefaciens transcriptional activator, initiates translation at a UUG codon and is a sequence-specific DNA-binding protein . J. Bacteriol. 172 : 1600 - 1608 . (10.1128/jb.172.3.1241-1249.1990) / J. Bacteriol. / virG, an Agrobacterium tumefaciens transcriptional activator, initiates translation at a UUG codon and is a sequence-specific DNA-binding protein by Pazour G. J. (1990)
Rogowsky , P. M. , T. J. Close , J. A. Chimera , J. J. Shaw , and C. I. Kado . 1987 . Regulation of the vir genes of Agrobacterium tumefaciens plasmid pTiC58 . J. Bacteriol. 169 : 5101 - 5112 . (10.1128/jb.169.11.5101-5112.1987) / J. Bacteriol. / Regulation of the vir genes of Agrobacterium tumefaciens plasmid pTiC58 by Rogowsky P. M. (1987)
Schmidhauser , T. J. , and D. R. Helinski . 1985 . Regions of broad-host-range plasmid RK2 involved in replication and stable maintenance in nine species of gram-negative bacteria . J. Bacteriol. 164 : 446 - 455 . (10.1128/jb.164.1.446-455.1985) / J. Bacteriol. / Regions of broad-host-range plasmid RK2 involved in replication and stable maintenance in nine species of gram-negative bacteria by Schmidhauser T. J. (1985)
Sciaky , D. , A. L. Montoya , and M. -D. Chiiton . 1978 . Fingerprints of Agrobacterium Ti plasmids . Plasmid 1 : 238 - 253 . (10.1016/0147-619X(78)90042-2) / Plasmid / Fingerprints of Agrobacterium Ti plasmids by Sciaky D. (1978)
Stachel , S. E. , E. Messens , M. Van Montagu , and P. Zambryski . 1985 . Identification of the signal molecules produced by wounded plant cells that activate T-DNA transfer in Agrobacterium tumefaciens . Nature (London) 318 : 624 - 629 . (10.1038/318624a0) / Nature (London) / Identification of the signal molecules produced by wounded plant cells that activate T-DNA transfer in Agrobacterium tumefaciens by Stachel S. E. (1985)
Stachel , S. E. , and E. W. Nester . 1986 . The genetic and transcriptional organization of the vir region of the A6 Ti plasmid of Agrobacterium tumefaciens . EMBO J. 5 : 1445 - 1454 . (10.1002/j.1460-2075.1986.tb04381.x) / EMBO J. / The genetic and transcriptional organization of the vir region of the A6 Ti plasmid of Agrobacterium tumefaciens by Stachel S. E. (1986)
Stachel , S. E. , E. W. Nester , and P. C. Zambryski . 1986 . A plant cell factor induces Agrobacterium tumefaciens vir gene expression . Proc. Natl. Acad. Sci. USA 83 : 379 - 383 . (10.1073/pnas.83.2.379) / Proc. Natl. Acad. Sci. USA / A plant cell factor induces Agrobacterium tumefaciens vir gene expression by Stachel S. E. (1986)
Stachel , S. E. , and P. C. Zambryski . 1986 . virA and virG control the plant-induced activation of the T-DNA transfer process of A. tumefaciens . Cell 46 : 325 - 333 . (10.1016/0092-8674(86)90653-7) / Cell / virA and virG control the plant-induced activation of the T-DNA transfer process of A. tumefaciens by Stachel S. E. (1986)
Veluthambi , K. , R. K. Jayaswal , and S. B. Gelvin . 1987 . Virulence genes A, G, and D mediate the double stranded border cleavage of T-DNA from the Agrobacterium Ti plasmid . Proc. Natl. Acad. Sci. USA 84 : 1881 - 1885 . (10.1073/pnas.84.7.1881) / Proc. Natl. Acad. Sci. USA / Virulence genes A, G, and D mediate the double stranded border cleavage of T-DNA from the Agrobacterium Ti plasmid by Veluthambi K. (1987)
Veluthambi , K. , M. Krishnan , J. H. Gould , R. H. Smith , and S. B. Gelvin . 1989 . Opines stimulate induction of the vir genes of the Agrobacterium tumefaciens Ti plasmid . J. Bacteriol. 171 : 3696 - 3703 . (10.1128/jb.171.7.3696-3703.1989) / J. Bacteriol. / Opines stimulate induction of the vir genes of the Agrobacterium tumefaciens Ti plasmid by Veluthambi K. (1989)
Winans , S. C. 1990 . Transcriptional induction of an Agrobacterium regulatory gene at tandem promoters by plant-released phenolics, phosphate starvation, and acidic growth media . J. Bacteriol. 172 : 2433 - 2438 . (10.1128/jb.172.5.2433-2438.1990) / J. Bacteriol. / Transcriptional induction of an Agrobacterium regulatory gene at tandem promoters by plant-released phenolics, phosphate starvation, and acidic growth media by Winans S. C. (1990)
Winans , S. C. , P. R. Ebert , S. E. Stachel , M. P. Gordon , and E. W. Nester . 1986 . A gene essential for Agrobacterium virulence is homologous to a family of positive regulatory loci . Proc. Natl. Acad. Sci. USA 83 : 8278 - 8282 . (10.1073/pnas.83.21.8278) / Proc. Natl. Acad. Sci. USA / A gene essential for Agrobacterium virulence is homologous to a family of positive regulatory loci by Winans S. C. (1986)
Winans , S. C. , R. A. Kerstetter , and E. W. Nester . 1988 . Transcriptional regulation of the virA and virG genes of Agrobacterium tumefaciens . J. Bacteriol. 170 : 4047 - 4054 . (10.1128/jb.170.9.4047-4054.1988) / J. Bacteriol. / Transcriptional regulation of the virA and virG genes of Agrobacterium tumefaciens by Winans S. C. (1988)
Yanofsky , M. F. , S. G. Porter , C. Young , L. A. Albright , M. P. Gordon , and E. W. Nester . 1986 . The virD operon of Agrobacterium tumefaciens encodes a site-specific endonuclease . Cell 47 : 471 - 477 . (10.1016/0092-8674(86)90604-5) / Cell / The virD operon of Agrobacterium tumefaciens encodes a site-specific endonuclease by Yanofsky M. F. (1986)

Dates

Type	When
Created	8 years, 9 months ago (Nov. 4, 2016, 2:18 p.m.)
Deposited	4 years ago (July 29, 2021, 1:54 p.m.)
Indexed	1 year ago (Aug. 11, 2024, 3:08 p.m.)
Issued	34 years, 6 months ago (Feb. 1, 1991)
Published	34 years, 6 months ago (Feb. 1, 1991)
Published Print	34 years, 6 months ago (Feb. 1, 1991)

Funders 0

None

BibTeX

@article{Chen_1991, title={Controlled expression of the transcriptional activator gene virG in Agrobacterium tumefaciens by using the Escherichia coli lac promoter}, volume={173}, ISSN={1098-5530}, url={http://dx.doi.org/10.1128/jb.173.3.1139-1144.1991}, DOI={10.1128/jb.173.3.1139-1144.1991}, number={3}, journal={Journal of Bacteriology}, publisher={American Society for Microbiology}, author={Chen, C Y and Winans, S C}, year={1991}, month=feb, pages={1139–1144} }

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      "journal-title": "Gene"
    },
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      "doi-asserted-by": "crossref",
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      "journal-title": "EMBO J."
    },
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      "key": "p_15",
      "unstructured": "Miller J. H. 1972. Experiments in molecular genetics. Cold Spring Harbor Laboratory Cold Spring Harbor N.Y."
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      "author": "Pazour G. J.",
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      "journal-title": "J. Bacteriol."
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      "journal-title": "J. Bacteriol."
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      "doi-asserted-by": "crossref",
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      "journal-title": "J. Bacteriol."
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      "volume": "1",
      "author": "Sciaky D.",
      "year": "1978",
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      "doi-asserted-by": "crossref",
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      "journal-title": "EMBO J."
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      "journal-title": "J. Bacteriol."
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      "journal-title": "J. Bacteriol."
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