DOI: 10.1128/jb.173.23.7719-7722.1991. Escherichia coli alkaline phosphatase fails to acquire disulfide bonds when retained in the cytoplasm

Escherichia coli alkaline phosphatase fails to acquire disulfide bonds when retained in the cytoplasm

10.1128/jb.173.23.7719-7722.1991

Crossref journal-article

American Society for Microbiology

Journal of Bacteriology (235)

Abstract

The cysteines of the Escherichia coli periplasmic enzyme alkaline phosphatase, which are involved in disulfide bonds in the native enzyme, were found to be fully reduced when the protein was retained in the cytoplasm. Under these circumstances the cysteines remained reduced for at least several minutes after the synthesis of the protein was completed. This contrasted with the normally exported protein, wherein disulfide bonds formed rapidly. Disulfide bond formation accompanied export and processing. The implications of these findings for the inactivity of the enzyme in the cytoplasm are discussed.

Bibliography

Derman, A. I., & Beckwith, J. (1991). Escherichia coli alkaline phosphatase fails to acquire disulfide bonds when retained in the cytoplasm. Journal of Bacteriology, 173(23), 7719â7722.

Authors 2

A I Derman (first)
J Beckwith (additional)

References 27 Referenced 156

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Dates

Type	When
Created	8 years, 10 months ago (Nov. 4, 2016, 1:58 p.m.)
Deposited	4 years, 1 month ago (July 29, 2021, 1:54 p.m.)
Indexed	1 month, 2 weeks ago (July 16, 2025, 7:54 a.m.)
Issued	33 years, 9 months ago (Dec. 1, 1991)
Published	33 years, 9 months ago (Dec. 1, 1991)
Published Print	33 years, 9 months ago (Dec. 1, 1991)

Funders 0

None

BibTeX

@article{Derman_1991, title={Escherichia coli alkaline phosphatase fails to acquire disulfide bonds when retained in the cytoplasm}, volume={173}, ISSN={1098-5530}, url={http://dx.doi.org/10.1128/jb.173.23.7719-7722.1991}, DOI={10.1128/jb.173.23.7719-7722.1991}, number={23}, journal={Journal of Bacteriology}, publisher={American Society for Microbiology}, author={Derman, A I and Beckwith, J}, year={1991}, month=dec, pages={7719–7722} }

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