DOI: 10.1128/jb.169.3.1291-1297.1987. Tn916-induced mutations in the hemolysin determinant affecting virulence of Listeria monocytogenes

Tn916-induced mutations in the hemolysin determinant affecting virulence of Listeria monocytogenes

10.1128/jb.169.3.1291-1297.1987

Crossref journal-article

American Society for Microbiology

Journal of Bacteriology (235)

Abstract

A genetic determinant essential for hemolysin production by Listeria monocytogenes has been inactivated by insertion of transposon Tn916 into L. monocytogenes DNA. The transposon was transferred by means of conjugation of a streptomycin-resistant L. monocytogenes recipient strain with Streptococcus faecalis CG110 on membrane filters. Among the tetracycline-resistant transconjugants, mutants were detected which had lost hemolytic activity. When tested in a mouse model, these mutants appeared to have lost the virulence that characterizes the parental strain. An extracellular protein of 58,000 apparent molecular weight was eliminated in the nonhemolytic mutants. In some of the mutants, the decrease in the production of the 58,000-dalton protein was accompanied by the production of a new protein of 49,000 apparent molecular weight. Hemolytic revertants regained the hemolytic phenotype and virulence and produced the extracellular protein that characterizes the recipient strain. Hybridization studies with Tn916 DNA indicated that the transposon is present in EcoRI and HindIII fragments of the nonhemolytic mutants. Single copies of Tn916 were detected in the chromosomal DNA of two of the three nonhemolytic mutants that were studied in detail. In hemolytic, tetracycline-sensitive revertants Tn916 appeared to be completely excised from the chromosome.

Bibliography

Kathariou, S., Metz, P., Hof, H., & Goebel, W. (1987). Tn916-induced mutations in the hemolysin determinant affecting virulence of Listeria monocytogenes. Journal of Bacteriology, 169(3), 1291â1297.

Authors 4

S Kathariou (first)
P Metz (additional)
H Hof (additional)
W Goebel (additional)

References 33 Referenced 289

Armstrong , B. A. , and C. P. Sword . 1966 . Electron microscopy of Listeria monocytogenes-infected mouse spleen . J. Bacteriol. 91 : 1346 - 1355 . (10.1128/jb.91.3.1346-1355.1966) / J. Bacteriol. / Electron microscopy of Listeria monocytogenes-infected mouse spleen by Armstrong B. A. (1966)
Audurier , A. , P. Pardon , J. Marly , and F. Lautier . 1980 . Experimental infection of mice with Listeria monocytogenes and Listeria innocua . Ann. Microbiol. (Paris) 131B : 47 - 57 . / Ann. Microbiol. (Paris) / Experimental infection of mice with Listeria monocytogenes and Listeria innocua by Audurier A. (1980)
Birnboim , H. C. , and J. Doly . 1979 . A rapid alkaline extraction procedure for screening recombinant plasmid DNA . Nucleic Acids Res. 7 : 1513 - 1523 . (10.1093/nar/7.6.1513) / Nucleic Acids Res. / A rapid alkaline extraction procedure for screening recombinant plasmid DNA by Birnboim H. C. (1979)
Flamm , R. K. , D. J. Hinrichs , and M. F. Thomashow . 1984 . Introduction of pAM,31 into Listeria monocytogenes by conjugation and homology between native L. monocytogenes plasmids . Infect. Immun. 44 : 157 - 161 . (10.1128/iai.44.1.157-161.1984) / Infect. Immun. / Introduction of pAM,31 into Listeria monocytogenes by conjugation and homology between native L. monocytogenes plasmids by Flamm R. K. (1984)
Franke , A. , and D. C Iewell . 1980 . Evidence for conjugal transfer of a Streptococcus faecalis transposon (Tn 916) from a chromosomal site in the absence of plasmid DNA . Cold Spring HIarbor Symp. Quant. Biol. 45 : 77 - 80 . (10.1101/SQB.1981.045.01.014) / Cold Spring HIarbor Symp. Quant. Biol. / Evidence for conjugal transfer of a Streptococcus faecalis transposon (Tn 916) from a chromosomal site in the absence of plasmid DNA by Franke A. (1980)
Franke , A. , and D. B. Clewell . 1981 . Evidence for a chroniosome-borne resistance transposon (Tn 916) in Streptococcus faecalis that is capable of "conjugal " transfer in the absence of a conjugative J. Bacteriol. 145 : 494 - 502 . (10.1128/jb.145.1.494-502.1981) / J. Bacteriol. / Evidence for a chroniosome-borne resistance transposon (Tn 916) in Streptococcus faecalis that is capable of "conjugal by Franke A. (1981)
Gailiard , J. L. , P. Berche , and P. Sansonetti . 1986 . Transposon mutagenesis as a tool to study the role of hemolysin in the virulence of Listeria monocytogenes . Infect. Immun. 52 : 50 - 55 . (10.1128/iai.52.1.50-55.1986) / Infect. Immun. / Transposon mutagenesis as a tool to study the role of hemolysin in the virulence of Listeria monocytogenes by Gailiard J. L. (1986)
Gawron-Burke , C. , and D. B. Clewell . 1982 . A transposon in Streptococcus faecalis with fertility properties . Nature (London) 300 : 281 - 284 . (10.1038/300281a0) / Nature (London) / A transposon in Streptococcus faecalis with fertility properties by Gawron-Burke C. (1982)
Gawron-Burke , C. , and D. B. Clewell . 1984 . Regeneration of insertionally inactivated streptococcal DNA fragments after excision of transposon Th 916 in Escherichia coli: strategy for targeting and cloning of genes from gram-positive bacteria . J. Bacteriol. 159 : 214 - 221 . (10.1128/jb.159.1.214-221.1984) / J. Bacteriol. / Regeneration of insertionally inactivated streptococcal DNA fragments after excision of transposon Th 916 in Escherichia coli: strategy for targeting and cloning of genes from gram-positive bacteria by Gawron-Burke C. (1984)
Ghosh B. K. and R. G. E. Murray. 1967. Fine structure of VOL. 169 1987 MUTATIONS IN L. MONOCYTOGENES HEMOLYSIN DETERMINANT
Groves , R. D. , and H. J. Welshimer . 1977 . Separation of pathogenic from apathogenic Listeria monocytogenes by three in vitro reactions . J. Clin. Microbiol. 5 : 559 - 563 . (10.1128/jcm.5.6.559-563.1977) / J. Clin. Microbiol. / Separation of pathogenic from apathogenic Listeria monocytogenes by three in vitro reactions by Groves R. D. (1977)
Hof , H. 1984 . Virulence of different strains of Listeria monocytogenes serovar 1/2a . Med. Microbiol. Immunol. 173 : 207 - 218 . (10.1007/BF02122112) / Med. Microbiol. Immunol. / Virulence of different strains of Listeria monocytogenes serovar 1/2a by Hof H. (1984)
Ike , Y. , H. Hashimoto , and D. B. Clewell . 1984 . Hemolysin of Streptococcus faecalis subspecies zymogenes contributes to virulence in mice . Infect. Immun. 45 : 528 - 530 . (10.1128/iai.45.2.528-530.1984) / Infect. Immun. / Hemolysin of Streptococcus faecalis subspecies zymogenes contributes to virulence in mice by Ike Y. (1984)
Jenkins , E. M. , A. N. Njoku-Obi , and E. W. Adams . 1964 . Purification of the soluble hemolysins of Listeria monocytogenes . J. Bacteriol. 88 : 418 - 424 . (10.1128/jb.88.2.418-424.1964) / J. Bacteriol. / Purification of the soluble hemolysins of Listeria monocytogenes by Jenkins E. M. (1964)
Kaufmann , S. H. E. 1984 . Acquired resistance to facultative intracellular bacteria: relationship between persistence, crossreactivity at the T-cell level, and capacity to stimulate cellular immunity of different Listeria strains . Infect. Immun. 45 : 234 - 241 . (10.1128/iai.45.1.234-241.1984) / Infect. Immun. / Acquired resistance to facultative intracellular bacteria: relationship between persistence, crossreactivity at the T-cell level, and capacity to stimulate cellular immunity of different Listeria strains by Kaufmann S. H. E. (1984)
Kaufmann , S. H. E. 1986 . Enumeration of Listeria monocytogenes-reactive L3T4 T cells activated during infection . Microb. Pathogenesis 1 : 249 - 260 . (10.1016/0882-4010(86)90049-5) / Microb. Pathogenesis / Enumeration of Listeria monocytogenes-reactive L3T4 T cells activated during infection by Kaufmann S. H. E. (1986)
Kehoe , M. , and K. N. Timmis . 1984 . Cloning and expression in Escherichia coli of the streptolysin 0 determinant from Streptococcus pyogenes: characterization of the cloned streptolysin 0 determinant and demonstration of the absence of substantial homology with determinants of other thiol-activated toxins . Infect. Immun. 43 : 804 - 810 . (10.1128/iai.43.3.804-810.1984) / Infect. Immun. / Cloning and expression in Escherichia coli of the streptolysin 0 determinant from Streptococcus pyogenes: characterization of the cloned streptolysin 0 determinant and demonstration of the absence of substantial homology with determinants of other thiol-activated toxins by Kehoe M. (1984)
Kingdon , G. C. , and C. P. Sword . 1970 . Effects of Listeria monocytogenes hemolysin on phagocytic cells and Iysosomes . Infect. Immun. 1 : 373 - 379 . (10.1128/iai.1.4.373-379.1970) / Infect. Immun. / Effects of Listeria monocytogenes hemolysin on phagocytic cells and Iysosomes by Kingdon G. C. (1970)
Kleckner , N. , J. Roth , and D. Botstein . 1977 . Genetic engineering in vivo using translocatable drug-resistance elements. New methods in bacterial genetics . J. Mol. Biol. 116 : 125 - 159 . (10.1016/0022-2836(77)90123-1) / J. Mol. Biol. / Genetic engineering in vivo using translocatable drug-resistance elements. New methods in bacterial genetics by Kleckner N. (1977)
Laemmli , U. K. 1970 . Cleavage of structural proteins during the assembly of the head of bacteriophage T4 . Nature (London) 227 : 680 - 685 . (10.1038/227680a0) / Nature (London) / Cleavage of structural proteins during the assembly of the head of bacteriophage T4 by Laemmli U. K. (1970)
Mackaness , G. B. 1962 . Cellular resistance to infection . J. Exp. Med. 116 : 381 - 417 . 1297 (10.1084/jem.116.3.381) / J. Exp. Med. / Cellular resistance to infection by Mackaness G. B. (1962)
Maniatis T. E. F. Fritsch and J. Sambrook. 1982. Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory Cold Spring Harbor N.Y.
Nicas , T. I. , and B. H. Iglewski . 1984 . Isolation and characterization of transposon-induced mutants of Pseudomonas aeruginosa deficient in production of exoenzyme S . Infect. Immun. 45 : 470 - 474 . (10.1128/iai.45.2.470-474.1984) / Infect. Immun. / Isolation and characterization of transposon-induced mutants of Pseudomonas aeruginosa deficient in production of exoenzyme S by Nicas T. I. (1984)
Nida , K. , and P. P. Cleary . 1983 . Insertional inactivation of streptolysin S expression in Streptococcus pyogenes . J. Bacteriol. 155 : 1156 - 1161 . (10.1128/jb.155.3.1156-1161.1983) / J. Bacteriol. / Insertional inactivation of streptolysin S expression in Streptococcus pyogenes by Nida K. (1983)
Rocourt , J. , J. M. Alonso , and H. P. R. Seeliger . 1983 . Virulence comparee des cinq groupes genomiques de Listeria monocytogenes (sensu lato) . Ann. Microbiol. (Paris) 134A : 359 - 364 . / Ann. Microbiol. (Paris) / Virulence comparee des cinq groupes genomiques de Listeria monocytogenes (sensu lato) by Rocourt J. (1983)
Seeliger H. P. R. 1961. Listeriosis. Hafner Publishing Co. New York.
Siddique , I. H. , I. F. Lin , and R. A. Chung . 1974 . Purification and characterization of hemolysin produced by Listeria monocytogenes . Am. J. Vet. Res. 35 : 289 - 296 . (10.2460/ajvr.1974.35.02.289) / Am. J. Vet. Res. / Purification and characterization of hemolysin produced by Listeria monocytogenes by Siddique I. H. (1974)
Skalka , B. , J. Smola , and K. Elischerova . 1982 . Routine test for in vitro differentiation of pathogenic and apathogenic Listeria monocytogenes . J. Clin. Microbiol. 15 : 503 - 507 . (10.1128/jcm.15.3.503-507.1982) / J. Clin. Microbiol. / Routine test for in vitro differentiation of pathogenic and apathogenic Listeria monocytogenes by Skalka B. (1982)
Smyth C. J. and J. L. Duncan. 1978. Thiol-activated (oxygenlabile) cytolysins p. 129-183. In J. Jeljaszewicz and T. Eadstrom (ed.) Bacterial toxins and cell membranes. Academic Press Inc. New York.
Southern , E. M. 1975 . Detection of specific sequences among DNA fragments separated by gel electrophoresis . J. Mol. Biol. 98 : 503 - 517 . (10.1016/S0022-2836(75)80083-0) / J. Mol. Biol. / Detection of specific sequences among DNA fragments separated by gel electrophoresis by Southern E. M. (1975)
Weiss , A. A. , E. L. Hewlett , G. D. Myers , and S. Falkow . 1983 . TnS-induced mutations affecting virulence factors of Bordetella pertussis . Infect. Immun. 42 : 33 - 41 . (10.1128/iai.42.1.33-41.1983) / Infect. Immun. / TnS-induced mutations affecting virulence factors of Bordetella pertussis by Weiss A. A. (1983)
Welshimer H. J. 1981. The genus Listeria and related organisms p. 1680-1687. In M. P. Starr H. Stolp H. G. Truper A. Balows and H. G. Schlegel (ed.) The prokaryotes vol. II. Springer-Verlag Berlin.
Wirsing , C. H. , H. Finger , and H. Hof . 1982 . Failure of killed Listeria monocytogenes vaccine to produce protective immunity . Nature (London) 297 : 233 - 234 . (10.1038/297233a0) / Nature (London) / Failure of killed Listeria monocytogenes vaccine to produce protective immunity by Wirsing C. H. (1982)

Dates

Type	When
Created	8 years, 10 months ago (Nov. 2, 2016, 6:52 p.m.)
Deposited	2 months, 3 weeks ago (June 11, 2025, 7:33 p.m.)
Indexed	2 weeks, 4 days ago (Aug. 19, 2025, 7:10 a.m.)
Issued	38 years, 6 months ago (March 1, 1987)
Published	38 years, 6 months ago (March 1, 1987)
Published Print	38 years, 6 months ago (March 1, 1987)

Funders 0

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BibTeX

@article{Kathariou_1987, title={Tn916-induced mutations in the hemolysin determinant affecting virulence of Listeria monocytogenes}, volume={169}, ISSN={1098-5530}, url={http://dx.doi.org/10.1128/jb.169.3.1291-1297.1987}, DOI={10.1128/jb.169.3.1291-1297.1987}, number={3}, journal={Journal of Bacteriology}, publisher={American Society for Microbiology}, author={Kathariou, S and Metz, P and Hof, H and Goebel, W}, year={1987}, month=mar, pages={1291–1297} }

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      "first-page": "47",
      "article-title": "Experimental infection of mice with Listeria monocytogenes and Listeria innocua",
      "volume": "131",
      "author": "Audurier A.",
      "year": "1980",
      "unstructured": "Audurier , A. , P. Pardon , J. Marly , and F. Lautier . 1980 . Experimental infection of mice with Listeria monocytogenes and Listeria innocua . Ann. Microbiol. (Paris) 131B : 47 - 57 .",
      "journal-title": "Ann. Microbiol. (Paris)"
    },
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      "key": "p_3",
      "doi-asserted-by": "crossref",
      "first-page": "1513",
      "DOI": "10.1093/nar/7.6.1513",
      "article-title": "A rapid alkaline extraction procedure for screening recombinant plasmid DNA",
      "volume": "7",
      "author": "Birnboim H. C.",
      "year": "1979",
      "unstructured": "Birnboim , H. C. , and J. Doly . 1979 . A rapid alkaline extraction procedure for screening recombinant plasmid DNA . Nucleic Acids Res. 7 : 1513 - 1523 .",
      "journal-title": "Nucleic Acids Res."
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      "key": "p_4",
      "doi-asserted-by": "crossref",
      "first-page": "157",
      "DOI": "10.1128/iai.44.1.157-161.1984",
      "article-title": "Introduction of pAM,31 into Listeria monocytogenes by conjugation and homology between native L. monocytogenes plasmids",
      "volume": "44",
      "author": "Flamm R. K.",
      "year": "1984",
      "unstructured": "Flamm , R. K. , D. J. Hinrichs , and M. F. Thomashow . 1984 . Introduction of pAM,31 into Listeria monocytogenes by conjugation and homology between native L. monocytogenes plasmids . Infect. Immun. 44 : 157 - 161 .",
      "journal-title": "Infect. Immun."
    },
    {
      "key": "p_5",
      "doi-asserted-by": "crossref",
      "first-page": "77",
      "DOI": "10.1101/SQB.1981.045.01.014",
      "article-title": "Evidence for conjugal transfer of a Streptococcus faecalis transposon (Tn 916) from a chromosomal site in the absence of plasmid DNA",
      "volume": "45",
      "author": "Franke A.",
      "year": "1980",
      "unstructured": "Franke , A. , and D. C Iewell . 1980 . Evidence for conjugal transfer of a Streptococcus faecalis transposon (Tn 916) from a chromosomal site in the absence of plasmid DNA . Cold Spring HIarbor Symp. Quant. Biol. 45 : 77 - 80 .",
      "journal-title": "Cold Spring HIarbor Symp. Quant. Biol."
    },
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      "key": "p_6",
      "doi-asserted-by": "crossref",
      "first-page": "494",
      "DOI": "10.1128/jb.145.1.494-502.1981",
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      "author": "Franke A.",
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      "journal-title": "J. Bacteriol."
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      "first-page": "50",
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      "author": "Gailiard J. L.",
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      "year": "1982",
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      "author": "Groves R. D.",
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    },
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      "DOI": "10.1007/BF02122112",
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      "volume": "173",
      "author": "Hof H.",
      "year": "1984",
      "unstructured": "Hof , H. 1984 . Virulence of different strains of Listeria monocytogenes serovar 1/2a . Med. Microbiol. Immunol. 173 : 207 - 218 .",
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    },
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      "author": "Ike Y.",
      "year": "1984",
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      "first-page": "418",
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      "volume": "88",
      "author": "Jenkins E. M.",
      "year": "1964",
      "unstructured": "Jenkins , E. M. , A. N. Njoku-Obi , and E. W. Adams . 1964 . Purification of the soluble hemolysins of Listeria monocytogenes . J. Bacteriol. 88 : 418 - 424 .",
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    },
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      "volume": "45",
      "author": "Kaufmann S. H. E.",
      "year": "1984",
      "unstructured": "Kaufmann , S. H. E. 1984 . Acquired resistance to facultative intracellular bacteria: relationship between persistence, crossreactivity at the T-cell level, and capacity to stimulate cellular immunity of different Listeria strains . Infect. Immun. 45 : 234 - 241 .",
      "journal-title": "Infect. Immun."
    },
    {
      "key": "p_16",
      "doi-asserted-by": "crossref",
      "first-page": "249",
      "DOI": "10.1016/0882-4010(86)90049-5",
      "article-title": "Enumeration of Listeria monocytogenes-reactive L3T4 T cells activated during infection",
      "volume": "1",
      "author": "Kaufmann S. H. E.",
      "year": "1986",
      "unstructured": "Kaufmann , S. H. E. 1986 . Enumeration of Listeria monocytogenes-reactive L3T4 T cells activated during infection . Microb. Pathogenesis 1 : 249 - 260 .",
      "journal-title": "Microb. Pathogenesis"
    },
    {
      "key": "p_17",
      "doi-asserted-by": "crossref",
      "first-page": "804",
      "DOI": "10.1128/iai.43.3.804-810.1984",
      "article-title": "Cloning and expression in Escherichia coli of the streptolysin 0 determinant from Streptococcus pyogenes: characterization of the cloned streptolysin 0 determinant and demonstration of the absence of substantial homology with determinants of other thiol-activated toxins",
      "volume": "43",
      "author": "Kehoe M.",
      "year": "1984",
      "unstructured": "Kehoe , M. , and K. N. Timmis . 1984 . Cloning and expression in Escherichia coli of the streptolysin 0 determinant from Streptococcus pyogenes: characterization of the cloned streptolysin 0 determinant and demonstration of the absence of substantial homology with determinants of other thiol-activated toxins . Infect. Immun. 43 : 804 - 810 .",
      "journal-title": "Infect. Immun."
    },
    {
      "key": "p_18",
      "doi-asserted-by": "crossref",
      "first-page": "373",
      "DOI": "10.1128/iai.1.4.373-379.1970",
      "article-title": "Effects of Listeria monocytogenes hemolysin on phagocytic cells and Iysosomes",
      "volume": "1",
      "author": "Kingdon G. C.",
      "year": "1970",
      "unstructured": "Kingdon , G. C. , and C. P. Sword . 1970 . Effects of Listeria monocytogenes hemolysin on phagocytic cells and Iysosomes . Infect. Immun. 1 : 373 - 379 .",
      "journal-title": "Infect. Immun."
    },
    {
      "key": "p_19",
      "doi-asserted-by": "crossref",
      "first-page": "125",
      "DOI": "10.1016/0022-2836(77)90123-1",
      "article-title": "Genetic engineering in vivo using translocatable drug-resistance elements. New methods in bacterial genetics",
      "volume": "116",
      "author": "Kleckner N.",
      "year": "1977",
      "unstructured": "Kleckner , N. , J. Roth , and D. Botstein . 1977 . Genetic engineering in vivo using translocatable drug-resistance elements. New methods in bacterial genetics . J. Mol. Biol. 116 : 125 - 159 .",
      "journal-title": "J. Mol. Biol."
    },
    {
      "key": "p_20",
      "doi-asserted-by": "crossref",
      "first-page": "680",
      "DOI": "10.1038/227680a0",
      "article-title": "Cleavage of structural proteins during the assembly of the head of bacteriophage T4",
      "volume": "227",
      "author": "Laemmli U. K.",
      "year": "1970",
      "unstructured": "Laemmli , U. K. 1970 . Cleavage of structural proteins during the assembly of the head of bacteriophage T4 . Nature (London) 227 : 680 - 685 .",
      "journal-title": "Nature (London)"
    },
    {
      "key": "p_21",
      "doi-asserted-by": "crossref",
      "first-page": "381",
      "DOI": "10.1084/jem.116.3.381",
      "article-title": "Cellular resistance to infection",
      "volume": "116",
      "author": "Mackaness G. B.",
      "year": "1962",
      "unstructured": "Mackaness , G. B. 1962 . Cellular resistance to infection . J. Exp. Med. 116 : 381 - 417 . 1297",
      "journal-title": "J. Exp. Med."
    },
    {
      "key": "p_22",
      "unstructured": "Maniatis T. E. F. Fritsch and J. Sambrook. 1982. Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory Cold Spring Harbor N.Y."
    },
    {
      "key": "p_23",
      "doi-asserted-by": "crossref",
      "first-page": "470",
      "DOI": "10.1128/iai.45.2.470-474.1984",
      "article-title": "Isolation and characterization of transposon-induced mutants of Pseudomonas aeruginosa deficient in production of exoenzyme S",
      "volume": "45",
      "author": "Nicas T. I.",
      "year": "1984",
      "unstructured": "Nicas , T. I. , and B. H. Iglewski . 1984 . Isolation and characterization of transposon-induced mutants of Pseudomonas aeruginosa deficient in production of exoenzyme S . Infect. Immun. 45 : 470 - 474 .",
      "journal-title": "Infect. Immun."
    },
    {
      "key": "p_24",
      "doi-asserted-by": "crossref",
      "first-page": "1156",
      "DOI": "10.1128/jb.155.3.1156-1161.1983",
      "article-title": "Insertional inactivation of streptolysin S expression in Streptococcus pyogenes",
      "volume": "155",
      "author": "Nida K.",
      "year": "1983",
      "unstructured": "Nida , K. , and P. P. Cleary . 1983 . Insertional inactivation of streptolysin S expression in Streptococcus pyogenes . J. Bacteriol. 155 : 1156 - 1161 .",
      "journal-title": "J. Bacteriol."
    },
    {
      "key": "p_25",
      "first-page": "359",
      "article-title": "Virulence comparee des cinq groupes genomiques de Listeria monocytogenes (sensu lato)",
      "volume": "134",
      "author": "Rocourt J.",
      "year": "1983",
      "unstructured": "Rocourt , J. , J. M. Alonso , and H. P. R. Seeliger . 1983 . Virulence comparee des cinq groupes genomiques de Listeria monocytogenes (sensu lato) . Ann. Microbiol. (Paris) 134A : 359 - 364 .",
      "journal-title": "Ann. Microbiol. (Paris)"
    },
    {
      "key": "p_26",
      "unstructured": "Seeliger H. P. R. 1961. Listeriosis. Hafner Publishing Co. New York."
    },
    {
      "key": "p_27",
      "doi-asserted-by": "crossref",
      "first-page": "289",
      "DOI": "10.2460/ajvr.1974.35.02.289",
      "article-title": "Purification and characterization of hemolysin produced by Listeria monocytogenes",
      "volume": "35",
      "author": "Siddique I. H.",
      "year": "1974",
      "unstructured": "Siddique , I. H. , I. F. Lin , and R. A. Chung . 1974 . Purification and characterization of hemolysin produced by Listeria monocytogenes . Am. J. Vet. Res. 35 : 289 - 296 .",
      "journal-title": "Am. J. Vet. Res."
    },
    {
      "key": "p_28",
      "doi-asserted-by": "crossref",
      "first-page": "503",
      "DOI": "10.1128/jcm.15.3.503-507.1982",
      "article-title": "Routine test for in vitro differentiation of pathogenic and apathogenic Listeria monocytogenes",
      "volume": "15",
      "author": "Skalka B.",
      "year": "1982",
      "unstructured": "Skalka , B. , J. Smola , and K. Elischerova . 1982 . Routine test for in vitro differentiation of pathogenic and apathogenic Listeria monocytogenes . J. Clin. Microbiol. 15 : 503 - 507 .",
      "journal-title": "J. Clin. Microbiol."
    },
    {
      "key": "p_29",
      "unstructured": "Smyth C. J. and J. L. Duncan. 1978. Thiol-activated (oxygenlabile) cytolysins p. 129-183. In J. Jeljaszewicz and T. Eadstrom (ed.) Bacterial toxins and cell membranes. Academic Press Inc. New York."
    },
    {
      "key": "p_30",
      "doi-asserted-by": "crossref",
      "first-page": "503",
      "DOI": "10.1016/S0022-2836(75)80083-0",
      "article-title": "Detection of specific sequences among DNA fragments separated by gel electrophoresis",
      "volume": "98",
      "author": "Southern E. M.",
      "year": "1975",
      "unstructured": "Southern , E. M. 1975 . Detection of specific sequences among DNA fragments separated by gel electrophoresis . J. Mol. Biol. 98 : 503 - 517 .",
      "journal-title": "J. Mol. Biol."
    },
    {
      "key": "p_31",
      "doi-asserted-by": "crossref",
      "first-page": "33",
      "DOI": "10.1128/iai.42.1.33-41.1983",
      "article-title": "TnS-induced mutations affecting virulence factors of Bordetella pertussis",
      "volume": "42",
      "author": "Weiss A. A.",
      "year": "1983",
      "unstructured": "Weiss , A. A. , E. L. Hewlett , G. D. Myers , and S. Falkow . 1983 . TnS-induced mutations affecting virulence factors of Bordetella pertussis . Infect. Immun. 42 : 33 - 41 .",
      "journal-title": "Infect. Immun."
    },
    {
      "key": "p_32",
      "unstructured": "Welshimer H. J. 1981. The genus Listeria and related organisms p. 1680-1687. In M. P. Starr H. Stolp H. G. Truper A. Balows and H. G. Schlegel (ed.) The prokaryotes vol. II. Springer-Verlag Berlin."
    },
    {
      "key": "p_33",
      "doi-asserted-by": "crossref",
      "first-page": "233",
      "DOI": "10.1038/297233a0",
      "article-title": "Failure of killed Listeria monocytogenes vaccine to produce protective immunity",
      "volume": "297",
      "author": "Wirsing C. H.",
      "year": "1982",
      "unstructured": "Wirsing , C. H. , H. Finger , and H. Hof . 1982 . Failure of killed Listeria monocytogenes vaccine to produce protective immunity . Nature (London) 297 : 233 - 234 .",
      "journal-title": "Nature (London)"
    }
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