Processing of the T-DNA of Agrobacterium tumefaciens generates border nicks and linear, single-stranded T-DNA
10.1128/jb.169.3.1046-1055.1987
Crossref journal-article
American Society for Microbiology
Journal of Bacteriology (235)
Abstract

Transfer and integration of a defined region (T-DNA) of the tumor-inducing (Ti) plasmid of Agrobacterium tumefaciens is essential for tumor formation. We used a physical assay to study structural changes induced in Agrobacterium T-DNA by cocultivation with plant cells. We show that nicks are introduced at unique, identical locations in each of the 24-base-pair imperfect direct repeats which flank the T-DNA and present evidence that a linear, single-stranded molecule is generated. We propose that these changes result from processing of the T-DNA for transfer and that they occur by a mechanism similar to DNA processing during conjugative DNA transfer between bacteria.

Bibliography

Albright, L. M., Yanofsky, M. F., Leroux, B., Ma, D. Q., & Nester, E. W. (1987). Processing of the T-DNA of Agrobacterium tumefaciens generates border nicks and linear, single-stranded T-DNA. Journal of Bacteriology, 169(3), 1046–1055.

Authors 5
  1. L M Albright (first)
  2. M F Yanofsky (additional)
  3. B Leroux (additional)
  4. D Q Ma (additional)
  5. E W Nester (additional)
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Dates
Type When
Created 8 years, 9 months ago (Nov. 2, 2016, 6:52 p.m.)
Deposited 4 years, 1 month ago (July 29, 2021, 1:36 p.m.)
Indexed 1 week, 4 days ago (Aug. 19, 2025, 6:27 a.m.)
Issued 38 years, 5 months ago (March 1, 1987)
Published 38 years, 5 months ago (March 1, 1987)
Published Print 38 years, 5 months ago (March 1, 1987)
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@article{Albright_1987, title={Processing of the T-DNA of Agrobacterium tumefaciens generates border nicks and linear, single-stranded T-DNA}, volume={169}, ISSN={1098-5530}, url={http://dx.doi.org/10.1128/jb.169.3.1046-1055.1987}, DOI={10.1128/jb.169.3.1046-1055.1987}, number={3}, journal={Journal of Bacteriology}, publisher={American Society for Microbiology}, author={Albright, L M and Yanofsky, M F and Leroux, B and Ma, D Q and Nester, E W}, year={1987}, month=mar, pages={1046–1055} }