Expression of bioluminescence by Escherichia coli containing recombinant Vibrio harveyi DNA
10.1128/jb.169.1.247-253.1987
Crossref journal-article
American Society for Microbiology
Journal of Bacteriology (235)
Abstract

When isogenic strains of Escherichia coli, RR1 (rec+) and HB101 (recA), were transformed with mapped recombinant plasmids known to contain Vibrio harveyi luciferase genes and large regions of DNA flanking on both sides, a small percentage (0.005%) of the colonies expressed high levels of luminescence (up to 10(12) quanta s-1 ml-1) in the absence of added aldehyde. The altered ability to express light was found to be due to a mutation in the host and not to an alteration in the recombinant DNA. When these bright colonies were cured of plasmid, they could be retransformed with cloned V. harveyi gene fragments in cis and in trans to yield luminescent colonies at 100% frequency. The maximum length of V. harveyi DNA required to produce light-emitting E. coli was shorter (6.3 kilobase pairs) than that required for expression of the V. fischeri system in E. coli. Cell extracts from bright clones contained wild-type levels of activity for the heteropolymeric (alpha beta) luciferase; fatty acid labeling revealed the presence of the three acylated polypeptides of the fatty acid reductase system which is involved in aldehyde biosynthesis for the luminescence reaction. The increased light emission in the mutant bacteria appeared to arise in part from production of higher levels of polycistronic mRNAs coding for luciferase.

Bibliography

Miyamoto, C., Byers, D., Graham, A. F., & Meighen, E. A. (1987). Expression of bioluminescence by Escherichia coli containing recombinant Vibrio harveyi DNA. Journal of Bacteriology, 169(1), 247–253.

Authors 4
  1. C Miyamoto (first)
  2. D Byers (additional)
  3. A F Graham (additional)
  4. E A Meighen (additional)
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Dates
Type When
Created 8 years, 10 months ago (Nov. 2, 2016, 4:28 p.m.)
Deposited 4 years, 1 month ago (July 29, 2021, 1:34 p.m.)
Indexed 1 month, 3 weeks ago (July 11, 2025, 6:41 a.m.)
Issued 38 years, 8 months ago (Jan. 1, 1987)
Published 38 years, 8 months ago (Jan. 1, 1987)
Published Print 38 years, 8 months ago (Jan. 1, 1987)
Funders 0

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@article{Miyamoto_1987, title={Expression of bioluminescence by Escherichia coli containing recombinant Vibrio harveyi DNA}, volume={169}, ISSN={1098-5530}, url={http://dx.doi.org/10.1128/jb.169.1.247-253.1987}, DOI={10.1128/jb.169.1.247-253.1987}, number={1}, journal={Journal of Bacteriology}, publisher={American Society for Microbiology}, author={Miyamoto, C and Byers, D and Graham, A F and Meighen, E A}, year={1987}, month=jan, pages={247–253} }