Glutamine synthetase-constitutive mutation affecting the glnALG upstream promoter of Escherichia coli
10.1128/jb.164.3.1032-1038.1985
Crossref journal-article
American Society for Microbiology
Journal of Bacteriology (235)
Abstract

The spontaneous gln-76 mutation of Escherichia coli (Osorio et al., Mol. Gen. Genet. 194:114-123, 1984) was previously shown to be responsible for the cis-dominant constitutive expression of the glnA gene in the absence of a glnG-glnF activator system. Nucleotide sequence analysis has now revealed that gln-76 is a single transversion T.A to A.T, an up-promoter mutation affecting the -10 region of glnAp1, the upstream promoter of the glnALG control region. Both, wild-type and gln-76 DNA control regions were cloned into the promoter-probe plasmid pKO1. Galactokinase activity determinations of cells carrying the fused plasmids showed 10-fold more effective expression mediated by gln-76 than by the glnA wild-type control region. Primer extension experiments with RNA from strains carrying the gln-76 control region indicated that the transcription initiation sites were the same in both the gln-76 mutant and the wild type.

Bibliography

León, P., Romero, D., Garciarrubio, A., Bastarrachea, F., & Covarrubias, A. A. (1985). Glutamine synthetase-constitutive mutation affecting the glnALG upstream promoter of Escherichia coli. Journal of Bacteriology, 164(3), 1032–1038.

Authors 5
  1. P León (first)
  2. D Romero (additional)
  3. A Garciarrubio (additional)
  4. F Bastarrachea (additional)
  5. A A Covarrubias (additional)
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Dates
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Created 5 years, 8 months ago (Jan. 3, 2020, 1:24 p.m.)
Deposited 4 years, 1 month ago (July 29, 2021, 1:31 p.m.)
Indexed 3 years, 5 months ago (March 29, 2022, 11:02 p.m.)
Issued 39 years, 9 months ago (Dec. 1, 1985)
Published 39 years, 9 months ago (Dec. 1, 1985)
Published Print 39 years, 9 months ago (Dec. 1, 1985)
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@article{Le_n_1985, title={Glutamine synthetase-constitutive mutation affecting the glnALG upstream promoter of Escherichia coli}, volume={164}, ISSN={1098-5530}, url={http://dx.doi.org/10.1128/jb.164.3.1032-1038.1985}, DOI={10.1128/jb.164.3.1032-1038.1985}, number={3}, journal={Journal of Bacteriology}, publisher={American Society for Microbiology}, author={León, P and Romero, D and Garciarrubio, A and Bastarrachea, F and Covarrubias, A A}, year={1985}, month=dec, pages={1032–1038} }