DOI: 10.1128/jb.164.2.571-577.1985. Comparative studies of the amino acid and nucleotide sequences of pilin derived from Pseudomonas aeruginosa PAK and PAO

Comparative studies of the amino acid and nucleotide sequences of pilin derived from Pseudomonas aeruginosa PAK and PAO

10.1128/jb.164.2.571-577.1985

Crossref journal-article

American Society for Microbiology

Journal of Bacteriology (235)

Abstract

The entire amino acid sequence for Pseudomonas aeruginosa PAO pilin was determined through peptide sequencing and from the complete nucleotide sequence encoding the pilin gene. The precursor PAO pilin is 149 amino acids in length which includes a 6-amino-acid positively charged leader sequence. Comparison of the amino acid sequences of pilin produced by P. aeruginosa PAO and PAK reveals a region of high homology corresponding to the leader peptide and residues 1 to 54 of the mature pilin. The amino acid sequence of the peptide encompassing the major antigenic determinant of PAK differs greatly from that of the equivalent region in PAO. The C-terminal regions of these proteins are semiconserved. Few major differences were found when the predicted secondary structures for PAO and PAK pilins were compared. Major nucleotide sequence variation between the equivalent restriction fragments from PAO and PAK occurred within the areas coding for the peptides containing the immunodominant site for PAK pilin and the C termini.

Bibliography

Sastry, P. A., Finlay, B. B., Pasloske, B. L., Paranchych, W., Pearlstone, J. R., & Smillie, L. B. (1985). Comparative studies of the amino acid and nucleotide sequences of pilin derived from Pseudomonas aeruginosa PAK and PAO. Journal of Bacteriology, 164(2), 571â577.

Authors 6

P A Sastry (first)
B B Finlay (additional)
B L Pasloske (additional)
W Paranchych (additional)
J R Pearlstone (additional)
L B Smillie (additional)

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Dates

Type	When
Created	5 years, 7 months ago (Jan. 3, 2020, 1:21 p.m.)
Deposited	2 years, 10 months ago (Oct. 9, 2022, 6:29 p.m.)
Indexed	1 month, 4 weeks ago (July 1, 2025, 9:34 a.m.)
Issued	39 years, 9 months ago (Nov. 1, 1985)
Published	39 years, 9 months ago (Nov. 1, 1985)
Published Print	39 years, 9 months ago (Nov. 1, 1985)

Funders 0

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BibTeX

@article{Sastry_1985, title={Comparative studies of the amino acid and nucleotide sequences of pilin derived from Pseudomonas aeruginosa PAK and PAO}, volume={164}, ISSN={1098-5530}, url={http://dx.doi.org/10.1128/jb.164.2.571-577.1985}, DOI={10.1128/jb.164.2.571-577.1985}, number={2}, journal={Journal of Bacteriology}, publisher={American Society for Microbiology}, author={Sastry, P A and Finlay, B B and Pasloske, B L and Paranchych, W and Pearlstone, J R and Smillie, L B}, year={1985}, month=nov, pages={571–577} }

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