DOI: 10.1128/jb.163.3.1215-1221.1985. Regulation of phenoxazinone synthase expression in Streptomyces antibioticus

Regulation of phenoxazinone synthase expression in Streptomyces antibioticus

10.1128/jb.163.3.1215-1221.1985

Crossref journal-article

American Society for Microbiology

Journal of Bacteriology (235)

Abstract

The cloned gene for the subunit of phenoxazinone synthase (PHS), an enzyme implicated in the biosynthesis of actinomycin in Streptomyces antibioticus, was used as a probe to study the regulation of the enzyme. The direction of transcription of the PHS gene was determined with end-labeled restriction fragments derived from the gene. Low-resolution S1 mapping revealed that transcription was initiated at a position which may lie within the SphI restriction site, which represents the limit of the cloned sequence. Northern blotting allowed the identification of the putative PHS message. This RNA appeared to be significantly larger than the size required to encode the PHS subunit. RNA dot blotting showed that the increase in PHS specific activity observed in cultures grown on antibiotic production medium, with galactose as a carbon source, was due in part to an increased production of PHS mRNA. PHS was also more stable than most cellular proteins and appeared to be protected against degradation under conditions in which most other proteins are broken down. This protective effect also contributed to the increase in PHS specific activity observed in S. antibioticus cultures grown on production medium. The repression of PHS synthesis by glucose was also reflective of a transcriptional control mechanism. At early time points postinoculation, PHS mRNA levels were lower in cultures grown on glucose as a carbon source than in cultures of the same age grown on galactose. mRNA levels presumably begin to increase only after all the glucose in the medium is utilized. The ability of 5-fluorouracil to stimulate PHS production in young cultures was also due to the synthesis of new mRNA for the enzyme.

Bibliography

Jones, G. H. (1985). Regulation of phenoxazinone synthase expression in Streptomyces antibioticus. Journal of Bacteriology, 163(3), 1215â1221.

Authors 1

G H Jones (first)

References 22 Referenced 22

Ceriotti , B. 1952 . A microchemical determination of desoxyribonucleic acid . J. Biol. Chem. 198 : 297 - 303 . (10.1016/S0021-9258(18)55583-6) / J. Biol. Chem. / A microchemical determination of desoxyribonucleic acid by Ceriotti B. (1952)
Chatterjee , S. , and C. Vining . 1982 . Intracellular adenosine triphosphate and cyclic adenosine 3',5'-monophosphate concentrations during derepression of actinomycin biosynthesis . Can. J. Microbiol. 28 : 1396 - 1399 . (10.1139/m82-207) / Can. J. Microbiol. / Intracellular adenosine triphosphate and cyclic adenosine 3',5'-monophosphate concentrations during derepression of actinomycin biosynthesis by Chatterjee S. (1982)
Choy , H. A. , and G. H. Jones . 1981 . Phenoxazinone synthase from Streptomyces antibioticus: purification of the large and small enzyme forms . Arch. Biochem. Biophys. 211 : 55 - 65 . (10.1016/0003-9861(81)90429-X) / Arch. Biochem. Biophys. / Phenoxazinone synthase from Streptomyces antibioticus: purification of the large and small enzyme forms by Choy H. A. (1981)
de Crombrugghe , B. , S. Busby , and H. Buc . 1984 . Cyclic AMP receptor protein: role in transcription activation . Science 224 : 831 - 838 . (10.1126/science.6372090) / Science / Cyclic AMP receptor protein: role in transcription activation by de Crombrugghe B. (1984)
Favaloro , J. , R. Treisman , and R. Kamen . 1980 . Transcription maps of polyoma virus-specific RNA: analysis by twodimensional nuclease S1 gel mapping . Methods Enzymol. 65 : 718 - 749 . (10.1016/S0076-6879(80)65070-8) / Methods Enzymol. / Transcription maps of polyoma virus-specific RNA: analysis by twodimensional nuclease S1 gel mapping by Favaloro J. (1980)
GaBo , M. , and E. Katz . 1972 . Regulation of secondary metabolite biosynthesis: catabolite repression of phenoxazinone synthase and actinomycin formation by glucose . J. Bacteriol. 109 : 659 - 667 . (10.1128/jb.109.2.659-667.1972) / J. Bacteriol. / Regulation of secondary metabolite biosynthesis: catabolite repression of phenoxazinone synthase and actinomycin formation by glucose by GaBo M. (1972)
Gladek , A. , and J. Zakrzewska . 1984 . Genome size of Streptomyces . FEMS Microbiol. Lett. 24 : 73 - 76 . (10.1111/j.1574-6968.1984.tb01247.x) / FEMS Microbiol. Lett. / Genome size of Streptomyces by Gladek A. (1984)
Jones , G. H. 1976 . RNA synthesis in Streptomyces antibioticus: in vitro effects of actinomycin and transcriptional inhibitors from 48-h cells . Biochemistry 15 : 3331 - 3341 . (10.1021/bi00660a025) / Biochemistry / RNA synthesis in Streptomyces antibioticus: in vitro effects of actinomycin and transcriptional inhibitors from 48-h cells by Jones G. H. (1976)
Jones , G. H. 1979 . Purification of RNA polymerase from actinomycin producing and nonproducing cells of Streptomyces antibioticus . Arch. Biochem. Biophys. 198 : 195 - 204 . (10.1016/0003-9861(79)90410-7) / Arch. Biochem. Biophys. / Purification of RNA polymerase from actinomycin producing and nonproducing cells of Streptomyces antibioticus by Jones G. H. (1979)
Jones , G. H. , and D. A. Hopwood . 1984 . Molecular cloning and expression of the phenoxazinone synthase gene from Streptomyces antibioticus . J. Biol. Chem. 259 : 14151 - 14157 . (10.1016/S0021-9258(18)89869-6) / J. Biol. Chem. / Molecular cloning and expression of the phenoxazinone synthase gene from Streptomyces antibioticus by Jones G. H. (1984)
Jones , G. H. , and D. A. Hopwood . 1984 . Activation of phenoxazinone synthase expression in Streptomyces lividans by cloned DNA sequences from Streptomyces antibioticus . J. Biol. Chem. 259 : 14158 - 14164 . (10.1016/S0021-9258(18)89870-2) / J. Biol. Chem. / Activation of phenoxazinone synthase expression in Streptomyces lividans by cloned DNA sequences from Streptomyces antibioticus by Jones G. H. (1984)
Jones , G. H. , and H. Weissbach . 1970 . RNA metabolism in Streptomyces antibioticus: effects of 5-fluorouracil on the appearance of phenoxazinone synthase . Arch. Biochem. Biophys. 137 : 558 - 573 . (10.1016/0003-9861(70)90473-X) / Arch. Biochem. Biophys. / RNA metabolism in Streptomyces antibioticus: effects of 5-fluorouracil on the appearance of phenoxazinone synthase by Jones G. H. (1970)
Katz , E. , C. J. Thompson , and D. A. Hopwood . 1983 . Cloning and expression of the tyrosinase gene from Streptomyces antibioticus in Streptomyces lividans . J. Gen. Microbiol. 129 : 2703 - 2714 . / J. Gen. Microbiol. / Cloning and expression of the tyrosinase gene from Streptomyces antibioticus in Streptomyces lividans by Katz E. (1983)
Katz , E. , and H. Weissbach . 1961 . Studies on the biosynthesis of actinomycin: enzymatic synthesis of the phenoxazinone chromaphore . J. Biol. Chem. 236:PC16-PC18. / J. Biol. Chem. 236:PC16-PC18. / Studies on the biosynthesis of actinomycin: enzymatic synthesis of the phenoxazinone chromaphore by Katz E. (1961)
Katz , E. , and H. Weissbach . 1982 . Biosynthesis of the actinomycin chromaphore; enzymatic conversion of 4-methyl-3-hydroxyanthranilic acid to actinocin . J. Biol. Chem. 237 : 882 - 886 . (10.1016/S0021-9258(18)60387-4) / J. Biol. Chem. / Biosynthesis of the actinomycin chromaphore; enzymatic conversion of 4-methyl-3-hydroxyanthranilic acid to actinocin by Katz E. (1982)
Kirby , K. S. , E. Fox-Carter , and M. Guest . 1967 . Isolation of deoxyribonucleic acid and ribosomal ribonucleic acid from bacteria . Biochem. J. 104 : 258 - 262 . (10.1042/bj1040258) / Biochem. J. / Isolation of deoxyribonucleic acid and ribosomal ribonucleic acid from bacteria by Kirby K. S. (1967)
Malpartida , F. , and D. A. Hopwood . 1984 . Molecular cloning of the whole biosynthetic pathway of a Streptomyces antibiotic and its expression in a heterologous host . Nature (London) 309 : 462 - 464 . (10.1038/309462a0) / Nature (London) / Molecular cloning of the whole biosynthetic pathway of a Streptomyces antibiotic and its expression in a heterologous host by Malpartida F. (1984)
Maniatis T. E. F. Fritsch and J. Sambrook. 1982. Molecular cloning a laboratory manual. Cold Spring Harbor Laboratory Cold Spring Harbor N.Y.
Marshall , R. , B. Redfield , E. Katz , and H. Weissbach . 1968 . Changes in phenoxazinone synthase activity during the growth cycle of Streptomyces antibioticus . Arch. Biochem. Biophys. 123 : 317 - 323 . (10.1016/0003-9861(68)90141-0) / Arch. Biochem. Biophys. / Changes in phenoxazinone synthase activity during the growth cycle of Streptomyces antibioticus by Marshall R. (1968)
Tautz , D. , and M. Renz . 1983 . An optimized freeze-squeeze method for the recovery of DNA fragments from agarose gels . Anal. Biochem. 132 : 14 - 19 . (10.1016/0003-2697(83)90419-0) / Anal. Biochem. / An optimized freeze-squeeze method for the recovery of DNA fragments from agarose gels by Tautz D. (1983)
Thomas , P. 1980 . Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose . Proc. Natl. Acad. Sci. U.S.A. 77 : 5201 - 5205 . (10.1073/pnas.77.9.5201) / Proc. Natl. Acad. Sci. U.S.A. / Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose by Thomas P. (1980)
Troost , T. , and E. Katz . 1979 . Phenoxazinone biosynthesis: accumulation of a precursor, 4-methy1-3-hydroxyanthranilic acid, by mutants of Streptomyces parvulus . J. Gen. Microbiol. 111 : 121 - 132 . (10.1099/00221287-111-1-121) / J. Gen. Microbiol. / Phenoxazinone biosynthesis: accumulation of a precursor, 4-methy1-3-hydroxyanthranilic acid, by mutants of Streptomyces parvulus by Troost T. (1979)

Dates

Type	When
Created	5 years, 8 months ago (Jan. 3, 2020, 1:20 p.m.)
Deposited	4 years, 1 month ago (July 29, 2021, 1:29 p.m.)
Indexed	3 months, 2 weeks ago (May 16, 2025, 9:30 a.m.)
Issued	40 years ago (Sept. 1, 1985)
Published	40 years ago (Sept. 1, 1985)
Published Print	40 years ago (Sept. 1, 1985)

Funders 0

None

BibTeX

@article{Jones_1985, title={Regulation of phenoxazinone synthase expression in Streptomyces antibioticus}, volume={163}, ISSN={1098-5530}, url={http://dx.doi.org/10.1128/jb.163.3.1215-1221.1985}, DOI={10.1128/jb.163.3.1215-1221.1985}, number={3}, journal={Journal of Bacteriology}, publisher={American Society for Microbiology}, author={Jones, G H}, year={1985}, month=sep, pages={1215–1221} }

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