Chloramphenicol-erythromycin resistance mutations in a 23S rRNA gene of Escherichia coli
10.1128/jb.162.2.551-557.1985
Crossref journal-article
American Society for Microbiology
Journal of Bacteriology (235)
Abstract

Two chloramphenicol resistance mutations were isolated in an Escherichia coli rRNA operon (rrnH) located on a multicopy plasmid. Both mutations also confer resistance to 14-atom lactone ring macrolide antibiotics, but they do not confer resistance to 16-atom lactone ring macrolide antibiotics or other inhibitors of the large ribosomal subunit. Classic genetic and recombinant DNA methods were used to map the two mutations to 154-base-pair regions of the 23S RNA genes. DNA sequencing of these regions revealed that chloramphenicol-erythromycin resistance results from a guanine-to-adenine transition at position 2057 of the 23S RNA genes of both independently isolated mutants. These mutations affect a region of 23S RNA strongly implicated in peptidyl transfer and known to interact with a variety of peptidyl transferase inhibitors.

Bibliography

Ettayebi, M., Prasad, S. M., & Morgan, E. A. (1985). Chloramphenicol-erythromycin resistance mutations in a 23S rRNA gene of Escherichia coli. Journal of Bacteriology, 162(2), 551–557.

Authors 3
  1. M Ettayebi (first)
  2. S M Prasad (additional)
  3. E A Morgan (additional)
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Dates
Type When
Created 5 years, 7 months ago (Jan. 3, 2020, 1:08 p.m.)
Deposited 4 years ago (July 29, 2021, 1:41 p.m.)
Indexed 1 month, 3 weeks ago (July 2, 2025, 2:48 p.m.)
Issued 40 years, 3 months ago (May 1, 1985)
Published 40 years, 3 months ago (May 1, 1985)
Published Print 40 years, 3 months ago (May 1, 1985)
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@article{Ettayebi_1985, title={Chloramphenicol-erythromycin resistance mutations in a 23S rRNA gene of Escherichia coli}, volume={162}, ISSN={1098-5530}, url={http://dx.doi.org/10.1128/jb.162.2.551-557.1985}, DOI={10.1128/jb.162.2.551-557.1985}, number={2}, journal={Journal of Bacteriology}, publisher={American Society for Microbiology}, author={Ettayebi, M and Prasad, S M and Morgan, E A}, year={1985}, month=may, pages={551–557} }