sn-Glycerol-3-phosphate auxotrophy of plsB strains of Escherichia coli: evidence that a second mutation, plsX, is required
10.1128/jb.160.2.711-717.1984
Crossref journal-article
American Society for Microbiology
Journal of Bacteriology (235)
Abstract

sn-Glycerol-3-phosphate auxotrophs defective in phospholipid synthesis contain a Km-defective sn-glycerol-3-phosphate acyltransferase. Detailed genetic analysis revealed that two mutations were required for the auxotrophic phenotype. One mutation, in the previously described plsB locus (sn-glycerol-3-phosphate acyltransferase structural gene), mapped near min 92 on the Escherichia coli linkage map. Isolation of Tn10 insertions cotransducible with the auxotrophy in phage P1 crosses revealed that a second mutation was required with plsB26 to confer the sn-glycerol-3-phosphate auxotrophic phenotype. This second locus, plsX, mapped between pyrC and purB near min 24 on the E. coli linkage map. Tn10 insertions near plsX allowed detailed mapping of the genetic loci in this region. A clockwise gene order putA pyrC flbA flaL flaT plsX fabD ptsG thiK purB was inferred from results of two- and three-factor crosses. Strains harboring the four possible configurations of the mutant and wild-type plsB and plsX loci were constructed. Isogenic plsB+ plsX+, plsB+ plsX50, and plsB26 plsX+ strains grew equally well on glucose minimal medium without sn-glycerol-3-phosphate. In addition, plsX or plsX+ had no apparent effect on sn-glycerol-3-phosphate acyltransferase activity measured in membrane preparations. The molecular basis for the plsX requirement for conferral of sn-glycerol-3-phosphate auxotrophy in these strains remains to be established.

Bibliography

Larson, T. J., Ludtke, D. N., & Bell, R. M. (1984). sn-Glycerol-3-phosphate auxotrophy of plsB strains of Escherichia coli: evidence that a second mutation, plsX, is required. Journal of Bacteriology, 160(2), 711–717.

Authors 3
  1. T J Larson (first)
  2. D N Ludtke (additional)
  3. R M Bell (additional)
References 44 Referenced 53
  1. Argast , M. , D. Ludtke , T. J. Silhavy , and W. Boos . 1978 . A second transport system for sn-glycero1-3-phosphate in Escherichia coli . J. Bacteriol. 136 : 1070 - 1083 . (10.1128/JB.136.3.1070-1083.1978) / J. Bacteriol. / A second transport system for sn-glycero1-3-phosphate in Escherichia coli by Argast M. (1978)
  2. Bachmann , B. J. 1983 . Linkage map of Escherichia coli K-12, edition 7 . Microbiol. Rev. 47 : 180 - 230 . (10.1128/mr.47.2.180-230.1983) / Microbiol. Rev. / Linkage map of Escherichia coli K-12, edition 7 by Bachmann B. J. (1983)
  3. Beckwith , J. R. ? A. B. Pardee , R. Austrian , and F. Jacob . 1962 . Coordination of the synthesis of the enzymes in the pyrimidine pathway of E. coli . J. Mol. Biol. 5 : 618 - 634 . (10.1016/S0022-2836(62)80090-4) / J. Mol. Biol. / Coordination of the synthesis of the enzymes in the pyrimidine pathway of E. coli by Beckwith J. R. (1962)
  4. Bell , R. M. 1974 . Mutants of Escherichia coli defective in membrane phospholipid synthesis: Macromolecular synthesis in an sn-glycerol 3-phosphate acyltransferase Km mutant . J. Bacteriol. 117 : 1065 - 1076 . (10.1128/jb.117.3.1065-1076.1974) / J. Bacteriol. / Mutants of Escherichia coli defective in membrane phospholipid synthesis: Macromolecular synthesis in an sn-glycerol 3-phosphate acyltransferase Km mutant by Bell R. M. (1974)
  5. Bell , R. M. 1975 . Mutants of Escherichia coli defective in membrane phospholipid synthesis. Properties of wild type and Km defective sn-glycero1-3-phosphate acyltransferase activities . J. Biol. Chem. 250 : 7147 - 7152 . (10.1016/S0021-9258(19)40921-6) / J. Biol. Chem. / Mutants of Escherichia coli defective in membrane phospholipid synthesis. Properties of wild type and Km defective sn-glycero1-3-phosphate acyltransferase activities by Bell R. M. (1975)
  6. Bell , R. M. , and J. E. Cronan , Jr. 1975 . Mutants of Escherichia coli defective in membrane phospholipid synthesis. Phenotypic suppression of sn-glycero1-3-phosphate acyltransferase Km mutants by loss offeedback inhibition ofthe biosynthetic sn-glycerol-3-phosphate dehydrogenase . J. Biol. Chem. 250 : 7153 - 7158 . (10.1016/S0021-9258(19)40922-8) / J. Biol. Chem. / Mutants of Escherichia coli defective in membrane phospholipid synthesis. Phenotypic suppression of sn-glycero1-3-phosphate acyltransferase Km mutants by loss offeedback inhibition ofthe biosynthetic sn-glycerol-3-phosphate dehydrogenase by Bell R. M. (1975)
  7. Bochner , B. R. , H. -C. Huang , G. L. Schieven , and B. N. Ames . 1980 . Positive selection for loss of tetracycline resistance . J. Bacteriol. 143 : 926 - 933 . (10.1128/jb.143.2.926-933.1980) / J. Bacteriol. / Positive selection for loss of tetracycline resistance by Bochner B. R. (1980)
  8. Bochner , B. R. , and M. A. Savageau . 1977 . Generalized indicator plates for genetic, metabolic, and taxanomic studies with microorganisms . Appl. Environ. Microbiol. 33 : 434 444. (10.1128/AEM.33.2.434-444.1977) / Appl. Environ. Microbiol. / Generalized indicator plates for genetic, metabolic, and taxanomic studies with microorganisms by Bochner B. R. (1977)
  9. Brass , J. M. , M. D. Manson , and T. J. Larson . 1984 . Transposon TnlO-dependent expression of the lamB gene in Escherichia coli . J. Bacteriol. 159 : 93 - 99 . (10.1128/JB.159.1.93-99.1984) / J. Bacteriol. / Transposon TnlO-dependent expression of the lamB gene in Escherichia coli by Brass J. M. (1984)
  10. Casadaban , M. 1976 . Transposition and fusion of the lac genes to selected promoters in Escherichia coli using bacteriophage lambda and Mu . J. Mol. Biol. 104 : 541 - 555 . (10.1016/0022-2836(76)90119-4) / J. Mol. Biol. / Transposition and fusion of the lac genes to selected promoters in Escherichia coli using bacteriophage lambda and Mu by Casadaban M. (1976)
  11. Clark , D. J. , and 0. Maal #e. 1967 . DNA replication and the division cycle in Escherichia coli . J. Mol. Biol. 23 : 99 - 112 . (10.1016/S0022-2836(67)80070-6) / J. Mol. Biol. / DNA replication and the division cycle in Escherichia coli by Clark D. J. (1967)
  12. Condamine , H. 1971 . Mutants des voies de biosynth 6se et de degradation de la proline chez E. coli K-12 . Ann. Inst. Pasteur (Paris) 120 : 9 - 22 . / Ann. Inst. Pasteur (Paris) / Mutants des voies de biosynth 6se et de degradation de la proline chez E. coli K-12 by Condamine H. (1971)
  13. Edgar , J. R. , and R. M. Bell . 1978 . Biosynthesis in Escherichia coli of sn-glycero1-3-phosphate, a precursor of phospholipid. Kinetic characterization of wild type and feedback-resistant forms of the biosynthetic sn-glycero1-3-phosphate dehydrogenase . J. Biol. Chern. 253 : 6354 - 6363 . (10.1016/S0021-9258(19)46941-X) / J. Biol. Chern. / Biosynthesis in Escherichia coli of sn-glycero1-3-phosphate, a precursor of phospholipid. Kinetic characterization of wild type and feedback-resistant forms of the biosynthetic sn-glycero1-3-phosphate dehydrogenase by Edgar J. R. (1978)
  14. Edgar , J. R. , and R. M. Bell . 1979 . Biosynthesis in Escherichia coli of sn-glycero1-3-phosphate, a precursor of phospholipid. Palmitoyl-CoA inhibition of the biosynthetic sn-glycero1-3- phosphate dehydrogenase . J. Biol. Chem. 254 : 1016 - 1021 . (10.1016/S0021-9258(17)34160-1) / J. Biol. Chem. / Biosynthesis in Escherichia coli of sn-glycero1-3-phosphate, a precursor of phospholipid. Palmitoyl-CoA inhibition of the biosynthetic sn-glycero1-3- phosphate dehydrogenase by Edgar J. R. (1979)
  15. Esmon , B. E. , C. R. Kensil , C. -H. C. Cheng , and M. Glaser . 1980 . Genetic analysis of Escherichia coli mutants defective in adenylate kinase and sn-glycerol 3-phosphate acyltransferase . J. Bacteriol. 141 : 405 - 408 . (10.1128/jb.141.1.405-408.1980) / J. Bacteriol. / Genetic analysis of Escherichia coli mutants defective in adenylate kinase and sn-glycerol 3-phosphate acyltransferase by Esmon B. E. (1980)
  16. Garwin , J. L. , A. L. Klages , and J. E. Cronan , Jr. 1980 . Structural, enzymatic, and genetic studies of 0-ketoacyl-acyl carrier protein synthetases I and II of Escherichia coli . J. Biol. Chem. 255 : 11949 - 11956 . (10.1016/S0021-9258(19)70226-9) / J. Biol. Chem. / Structural, enzymatic, and genetic studies of 0-ketoacyl-acyl carrier protein synthetases I and II of Escherichia coli by Garwin J. L. (1980)
  17. Glaser , M. , W. Nulty , and P. R. Vagelos . 1975 . Role of adenylate kinase in the regulation of macromolecular biosynthesis in a putative mutant of Escherichia coli defective in membrane phospholipid biosynthesis . J. Bacteriol. 123 : 128 - 136 . (10.1128/jb.123.1.128-136.1975) / J. Bacteriol. / Role of adenylate kinase in the regulation of macromolecular biosynthesis in a putative mutant of Escherichia coli defective in membrane phospholipid biosynthesis by Glaser M. (1975)
  18. Goelz , S. E. , and J. E. Cronan , Jr. 1981 . Adenylate kinase of Escherichia coli. Evidence for a functional interaction in phospholipid synthesis . Biochemistry 21 : 189 - 195 . (10.1021/bi00530a032) / Biochemistry / Adenylate kinase of Escherichia coli. Evidence for a functional interaction in phospholipid synthesis by Goelz S. E. (1981)
  19. Green , P. R. , A. H. Merrill , Jr. , apd R. M. Bell . 1981 . Membrane phospholipid synthesis in Escherichia coli. Purification, reconstitution, and characterization of sn-glycero1-3-phosphate acyltransferase . J. Biol. Chem. 256 : 11151 - 11159 . (10.1016/S0021-9258(19)68570-4) / J. Biol. Chem. / Membrane phospholipid synthesis in Escherichia coli. Purification, reconstitution, and characterization of sn-glycero1-3-phosphate acyltransferase by Green P. R. (1981)
  20. Greener , A. , and C. W. Hill . 1980 . Identification of a novel genetic element in Escherichia coli K-12 . J. Bacteriol. 144 : 312 - 321 . (10.1128/jb.144.1.312-321.1980) / J. Bacteriol. / Identification of a novel genetic element in Escherichia coli K-12 by Greener A. (1980)
  21. Hayashi , S. , J. P. Koch , and E. C. C. Lin . 1964 . Active transport of L-a-glycerophosphate in Escherichia coli . J. Biol. Chem. 239 : 3098 - 3105 . (10.1016/S0021-9258(18)93858-5) / J. Biol. Chem. / Active transport of L-a-glycerophosphate in Escherichia coli by Hayashi S. (1964)
  22. Huss , R. J. , and M. Glaser . 1983 . Identification and purification of an adenylate kinase-associated protein that influences the thermolability of adenylate kinase from a temperature-sensitive adk mutant of Escherichia coli . J. Biol. Chem. 258 : 13370 - 13376 . (10.1016/S0021-9258(17)44126-3) / J. Biol. Chem. / Identification and purification of an adenylate kinase-associated protein that influences the thermolability of adenylate kinase from a temperature-sensitive adk mutant of Escherichia coli by Huss R. J. (1983)
  23. Imamura , N. , and H. Nakayama . 1982 . thiK and thiL loci of Escherichia coli . J. Bacteriol. 151 : 708 - 717 . (10.1128/JB.151.2.708-717.1982) / J. Bacteriol. / thiK and thiL loci of Escherichia coli by Imamura N. (1982)
  24. Komeda , Y. 1982 . Fusions of flagellar operons to lactose genes on a Mu lac bacteriophage . J. Bacteriol. 150 : 16 - 26 . (10.1128/jb.150.1.16-26.1982) / J. Bacteriol. / Fusions of flagellar operons to lactose genes on a Mu lac bacteriophage by Komeda Y. (1982)
  25. Komeda , Y. , M. Silverman , P. Matsumura , and M. Simon . 1978 . Genes for the hook-basal body proteins of the flagellar aparatus in Escherichia coli . J. Bacteriol. 134 : 655 - 667 . (10.1128/JB.134.2.655-667.1978) / J. Bacteriol. / Genes for the hook-basal body proteins of the flagellar aparatus in Escherichia coli by Komeda Y. (1978)
  26. Larson , T. J. , V. A. Lightner , P. R. Green , P. Modrich , and R. M. Bell . 1980 . Membrane phospholipid synthesis in Escherichia coli. Identification of the sn-glycero1-3-phosphate acyltransferase as the plsB gene product . J. Biol. Chem. 255 : 9421 - 9426 . (10.1016/S0021-9258(19)70579-1) / J. Biol. Chem. / Membrane phospholipid synthesis in Escherichia coli. Identification of the sn-glycero1-3-phosphate acyltransferase as the plsB gene product by Larson T. J. (1980)
  27. Lightner , V. A. , R. M. Bell , and P. Modrich . 1983 . The DNA sequences encoding pisB and dgk loci of Escherichia coli . J. Biol. Chem. 258 : 10856 - 10861 . (10.1016/S0021-9258(17)44354-7) / J. Biol. Chem. / The DNA sequences encoding pisB and dgk loci of Escherichia coli by Lightner V. A. (1983)
  28. Lightner V. A. T. J. Larson P. Tailleur G. D. Kantor C. R. H. Raetz R. M. Bell and P. Modrich. Membrane phospholipid synthesis in Escherichia coli. Cloning of a structural gene (plsB) of the sn-glycero1-3-phosphate acyltransferase. J. Biol. Chem. 255:9413-9420. (10.1016/S0021-9258(19)70578-X)
  29. Lin , E. C. C. 1976 . Glycerol dissimilation and its regulation in bacteria. Annu . Rev. Microbiol. 30 : 535 - 578 . (10.1146/annurev.mi.30.100176.002535) / Rev. Microbiol. / Glycerol dissimilation and its regulation in bacteria. Annu by Lin E. C. C. (1976)
  30. Loomis , W. F. , and B. Magasanik . 1965 . Genetic control of catabolite repression of the lac operon in Escherichia coli . Biochem. Biophys. Res. Commun. 20 : 230 - 234 . (10.1016/0006-291X(65)90351-7) / Biochem. Biophys. Res. Commun. / Genetic control of catabolite repression of the lac operon in Escherichia coli by Loomis W. F. (1965)
  31. Lowry , 0. H. , N. J. Rosebrough , A. L. Farr , and R. J. Randall . 1951 . Protein measurement with the Folin phenol reagent . J. Biol. Chem. 193 : 265 - 275 . (10.1016/S0021-9258(19)52451-6) / J. Biol. Chem. / Protein measurement with the Folin phenol reagent by Lowry 0. (1951)
  32. Ludtke , D. , T. J. Larson , C. Beck , and W. Boos . 1982 . Only one gene is necessary for the glpT-dependent transport of snglycero1-3-phosphate in Escherichia coli . Mol. Gen. Genet. 186 : 540 - 547 . (10.1007/BF00337962) / Mol. Gen. Genet. / Only one gene is necessary for the glpT-dependent transport of snglycero1-3-phosphate in Escherichia coli by Ludtke D. (1982)
  33. Maloy , S. R. , and W. D. Nunn . 1981 . Selection for loss of tetracycline resistance by Escherichia coli . J. Bacteriol. 145 : 1110 - 1112 . (10.1128/JB.145.2.1110-1111.1981) / J. Bacteriol. / Selection for loss of tetracycline resistance by Escherichia coli by Maloy S. R. (1981)
  34. Miller J. H. 1972. Experiments in molecular genetics. Cold Spring Harbor Laboratory Cold Spring Harbor N.Y.
  35. Parkinson , J. S. 1976 . cheA, cheB, and cheC genes of Escherichia coli and their role in chemotaxis . J. Bacteriol. 126 : 758 - 770 . (10.1128/JB.126.2.758-770.1976) / J. Bacteriol. / cheA, cheB, and cheC genes of Escherichia coli and their role in chemotaxis by Parkinson J. S. (1976)
  36. Raetz , C. R. H. 1978 . Enzymology, genetics, and regulation of membrane phospholipid synthesis in Escherichia coli . Microbiol. Rev. 42 : 614 - 659 . (10.1128/mr.42.3.614-659.1978) / Microbiol. Rev. / Enzymology, genetics, and regulation of membrane phospholipid synthesis in Escherichia coli by Raetz C. R. H. (1978)
  37. Raetz , C. R. H. , and K. F. Newman . 1978 . Neutral lipid accumulation in the membranes of Escherichia coli mutants lacking diglyceride kinase . J. Biol. Chem. 253 : 3882 - 3887 . (10.1016/S0021-9258(17)34773-7) / J. Biol. Chem. / Neutral lipid accumulation in the membranes of Escherichia coli mutants lacking diglyceride kinase by Raetz C. R. H. (1978)
  38. Rock , C. O. , and S. Jackowski . 1982 . Regulation of phospholipid synthesis in Escherichia coli. Composition of the acyl-acyl carrier protein pool in vivo . J. Biol. Chem. 257 : 10759 - 10765 . (10.1016/S0021-9258(18)33888-2) / J. Biol. Chem. / Regulation of phospholipid synthesis in Escherichia coli. Composition of the acyl-acyl carrier protein pool in vivo by Rock C. O. (1982)
  39. Schweizer , H. , M. Argast , and W. Boos . 1982 . Characterization of a binding protein-dependent transport system for sn-glycerol-3-phosphate in Escherichia coli that is part of the pho regulon . J. Bacteriol. 150 : 1154 - 1163 . (10.1128/jb.150.3.1154-1163.1982) / J. Bacteriol. / Characterization of a binding protein-dependent transport system for sn-glycerol-3-phosphate in Escherichia coli that is part of the pho regulon by Schweizer H. (1982)
  40. Schweizer , H. , and W. Boos . 1983 . Cloning of the ugp region containing the structural genes for the pho regulon-dependent sn-glycero1-3-phosphate transport system of Escherichia coli . Mol. Gen. Genet. 192 : 177 - 186 . (10.1007/BF00327664) / Mol. Gen. Genet. / Cloning of the ugp region containing the structural genes for the pho regulon-dependent sn-glycero1-3-phosphate transport system of Escherichia coli by Schweizer H. (1983)
  41. Semple , K. S. , and D. F. Silbert . 1975 . Mapping of the fabD locus for fatty acid biosynthesis in Escherichia coli . J. Bacteriol. 121 : 1036 - 1046 . (10.1128/jb.121.3.1036-1046.1975) / J. Bacteriol. / Mapping of the fabD locus for fatty acid biosynthesis in Escherichia coli by Semple K. S. (1975)
  42. Shuman , H. A. 1982 . Active transport of maltose in Escherichia coli K-12. Role of the periplasmic maltose-binding protein and evidence for a substrate recognition site in the cytoplasmic membrane . J. Biol. Chem. 257 : 5455 - 5461 . (10.1016/S0021-9258(19)83799-7) / J. Biol. Chem. / Active transport of maltose in Escherichia coli K-12. Role of the periplasmic maltose-binding protein and evidence for a substrate recognition site in the cytoplasmic membrane by Shuman H. A. (1982)
  43. van de Putte , P. , R. Plasterk , and A. Kuipers . 1984 . A Mu gin complementing function and an invertible DNA region in Escherichia coli K-12 are situated on the genetic element e14 . J. Bacteriol. 158 : 517 - 522 . (10.1128/jb.158.2.517-522.1984) / J. Bacteriol. / A Mu gin complementing function and an invertible DNA region in Escherichia coli K-12 are situated on the genetic element e14 by van de Putte P. (1984)
  44. Wu , T. T. 1966 . A model for three-point analysis of random general transduction . Genetics 54 : 405 - 410 . (10.1093/genetics/54.2.405) / Genetics / A model for three-point analysis of random general transduction by Wu T. T. (1966)
Dates
Type When
Created 5 years, 7 months ago (Jan. 3, 2020, 1:02 p.m.)
Deposited 4 years, 1 month ago (July 29, 2021, 1:40 p.m.)
Indexed 1 year, 7 months ago (Jan. 26, 2024, 12:23 a.m.)
Issued 40 years, 10 months ago (Nov. 1, 1984)
Published 40 years, 10 months ago (Nov. 1, 1984)
Published Print 40 years, 10 months ago (Nov. 1, 1984)
Funders 0

None

@article{Larson_1984, title={sn-Glycerol-3-phosphate auxotrophy of plsB strains of Escherichia coli: evidence that a second mutation, plsX, is required}, volume={160}, ISSN={1098-5530}, url={http://dx.doi.org/10.1128/jb.160.2.711-717.1984}, DOI={10.1128/jb.160.2.711-717.1984}, number={2}, journal={Journal of Bacteriology}, publisher={American Society for Microbiology}, author={Larson, T J and Ludtke, D N and Bell, R M}, year={1984}, month=nov, pages={711–717} }