DNA sequence and transcriptional organization of essential cell division genes ftsQ and ftsA of Escherichia coli: evidence for overlapping transcriptional units
10.1128/jb.160.2.546-555.1984
Crossref journal-article
American Society for Microbiology
Journal of Bacteriology (235)
Abstract

The DNA sequence of a cloned segment of the Escherichia coli chromosome containing ftsQ, ftsA, and part of the ftsZ gene was determined and interpreted for genetic complementation and promoter fusion data for the region. The contiguous genes ftsQ, ftsA, and ftsZ were transcribed in the same direction (clockwise on the genetic map) and each had at least one associated promoter which allowed it to be transcribed independently of neighboring genes. ftsA and ftsZ possessed promoters within the coding sequences of the juxtaposed upstream structural genes, and a promoter element for ftsA was surrounded by a region of twofold symmetry which corresponded closely to a symmetrical element in the region of a putative ftsZ promoter. The structural gene of ftsQ consisted of 838 nucleotides, encoding a 276-residue amino acid polypeptide of molecular weight 31,400; the structural gene of ftsA consisted of 1,260 nucleotides, encoding a 420-residue amino acid polypeptide of molecular weight 45,400. The observation that the termination codon of ftsQ overlaps with a potential initiation codon for ftsA suggested that these two genes may be translationally coupled when transcription is initiated upstream of the ftsQ coding sequence.

Bibliography

Robinson, A. C., Kenan, D. J., Hatfull, G. F., Sullivan, N. F., Spiegelberg, R., & Donachie, W. D. (1984). DNA sequence and transcriptional organization of essential cell division genes ftsQ and ftsA of Escherichia coli: evidence for overlapping transcriptional units. Journal of Bacteriology, 160(2), 546–555.

Authors 6
  1. A C Robinson (first)
  2. D J Kenan (additional)
  3. G F Hatfull (additional)
  4. N F Sullivan (additional)
  5. R Spiegelberg (additional)
  6. W D Donachie (additional)
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Dates
Type When
Created 5 years, 7 months ago (Jan. 3, 2020, 6:53 a.m.)
Deposited 4 years, 1 month ago (July 29, 2021, 9:40 a.m.)
Indexed 3 weeks, 6 days ago (Aug. 6, 2025, 9:19 a.m.)
Issued 40 years, 10 months ago (Nov. 1, 1984)
Published 40 years, 10 months ago (Nov. 1, 1984)
Published Print 40 years, 10 months ago (Nov. 1, 1984)
Funders 0

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@article{Robinson_1984, title={DNA sequence and transcriptional organization of essential cell division genes ftsQ and ftsA of Escherichia coli: evidence for overlapping transcriptional units}, volume={160}, ISSN={1098-5530}, url={http://dx.doi.org/10.1128/jb.160.2.546-555.1984}, DOI={10.1128/jb.160.2.546-555.1984}, number={2}, journal={Journal of Bacteriology}, publisher={American Society for Microbiology}, author={Robinson, A C and Kenan, D J and Hatfull, G F and Sullivan, N F and Spiegelberg, R and Donachie, W D}, year={1984}, month=nov, pages={546–555} }